Artículo
Coronato, S.; Coto, C. "Presence of mycoplasmas in cell cultures in Argentina " (1987) Revista Argentina de microbiología. 19(4):165-172
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Abstract:
The presence of mycoplasmas in cell cultures used for virus propagation in Argentine laboratories, was studied. Samples were obtained from laboratories located in the city of Buenos Aires and provinces of Córdoba and Buenos Aires. The fluorescent stain Hoechst 33258, which specifically binds to DNA, was used for mycoplasma testing. Thirty three (65%) of 51 analyzed cell cultures (48 established lines and 3 primary cultures), were found contaminated despite that all laboratories were well equipped to work under sterile conditions. The high rate of infection observed let us conclude that the risk to work with mycoplasma contaminated cultures was not properly appreciated.
Registro:
| Documento: | Artículo |
| Título: | Presence of mycoplasmas in cell cultures in Argentina |
| Autor: | Coronato, S.; Coto, C. |
| Filiación: | Laboratorio de Virología, Facultad de Ciencias Exacias y Naturales, Universidad de Buenos Aires, Argentina |
| Palabras clave: | bisbenzimide; animal; article; cell culture; culture technique; human; isolation and purification; microbiology; Mycoplasmatales; staining; standard; virus culture; Animals; Bisbenzimide; Cells, Cultured; Culture Techniques; Humans; Mycoplasmatales; Staining and Labeling; Virus Cultivation |
| Año: | 1987 |
| Volumen: | 19 |
| Número: | 4 |
| Página de inicio: | 165 |
| Página de fin: | 172 |
| Título revista: | Revista Argentina de microbiología |
| Título revista abreviado: | Rev. Argent. Microbiol. |
| ISSN: | 03257541 |
| CAS: | Bisbenzimide, 23491-45-4 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v19_n4_p165_Coronato |
Citas:
---------- APA ----------
Coronato, S. & Coto, C. (1987) . Presence of mycoplasmas in cell cultures in Argentina . Revista Argentina de microbiología, 19(4), 165-172.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v19_n4_p165_Coronato [ ]
---------- CHICAGO ----------
Coronato, S., Coto, C. "Presence of mycoplasmas in cell cultures in Argentina " . Revista Argentina de microbiología 19, no. 4 (1987) : 165-172.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v19_n4_p165_Coronato [ ]
---------- MLA ----------
Coronato, S., Coto, C. "Presence of mycoplasmas in cell cultures in Argentina " . Revista Argentina de microbiología, vol. 19, no. 4, 1987, pp. 165-172.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v19_n4_p165_Coronato [ ]
---------- VANCOUVER ----------
Coronato, S., Coto, C. Presence of mycoplasmas in cell cultures in Argentina . Rev. Argent. Microbiol. 1987;19(4):165-172.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v19_n4_p165_Coronato [ ]
