Artículo
Abstract:
Crude extracts from fresh green leaves of Melia azedarach L contain an antiviral factor (FAV) able to inhibit the replication of several animal viruses, e.g. Polio, VSV, HSV, FMDV, Sindbis, Junín, Pichinde and Tacaribe in Vero or BHK-21 cells. Crude preparations were subjected to different steps of purification like chromatography on Sephadex G-100 and DEAE-Sephadex. The antiviral activity of G-100 and DEAE fractions was fully conserved, whereas contaminating proteins were lost. Two types of cytotoxicity tests were performed with the different fractions. Two-fold serial dilutions of each of them were added to preformed monolayers of Vero or BHK-21 cells and cellular viability was tested. While crude extracts were toxic at low dilutions (less than or equal to 1:10), G-100 and DEAE fractions were not. The other cytotoxicity assay consisted in seeding the cells in the presence of different concentrations of each fraction. G-100 fraction affected cell growth at low dilutions (less than or equal to 1:5), while DEAE fraction did not. It should be remarked that the purification procedure rendered a partial purified DEAE fraction with an increased specific activity (antiviral activity/mg of protein). It is concluded that an antiviral factor devoid of toxicity exists in M. azedarach L extracts, which exhibited a broad spectrum of antiviral activity.
Registro:
| Documento: | Artículo |
| Título: | Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. |
| Autor: | Andrei, G.M.; Coto, C.E.; de Torres, R.A. |
| Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
| Palabras clave: | antivirus agent; plant extract; Arenavirus; Argentina; article; cell division; cell survival; drug effect; Herpes virus; medicinal plant; physiology; Poliomyelitis virus; Sindbis virus; tree; virus replication; Antiviral Agents; Arenaviruses, New World; Argentina; Cell Division; Cell Survival; Herpesviridae; Plant Extracts; Plants, Medicinal; Poliovirus; Sindbis Virus; Trees; Virus Replication |
| Año: | 1985 |
| Volumen: | 17 |
| Número: | 4 |
| Página de inicio: | 187 |
| Página de fin: | 194 |
| Título revista: | Revista Argentina de microbiología |
| Título revista abreviado: | Rev. Argent. Microbiol. |
| ISSN: | 03257541 |
| CAS: | Antiviral Agents; Plant Extracts |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei |
Citas:
---------- APA ----------
Andrei, G.M., Coto, C.E. & de Torres, R.A. (1985) . Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. . Revista Argentina de microbiología, 17(4), 187-194.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei [ ]
---------- CHICAGO ----------
Andrei, G.M., Coto, C.E., de Torres, R.A. "Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. " . Revista Argentina de microbiología 17, no. 4 (1985) : 187-194.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei [ ]
---------- MLA ----------
Andrei, G.M., Coto, C.E., de Torres, R.A. "Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. " . Revista Argentina de microbiología, vol. 17, no. 4, 1985, pp. 187-194.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei [ ]
---------- VANCOUVER ----------
Andrei, G.M., Coto, C.E., de Torres, R.A. Assays of cytotoxicity and antiviral activity of crude and semipurified extracts of green leaves of Melia azedarach L. . Rev. Argent. Microbiol. 1985;17(4):187-194.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03257541_v17_n4_p187_Andrei [ ]
