Artículo
Abstract:
Protein S has an essential anticoagulant function acting as activated Protein C cofactor and forming an equimolecular complex with it. In the presence of calcium this complex regulates the coagulation process inactivating thrombin activated factors V and VIII by proteolysis. In plasma there are two different forms: a) free Protein S which acts as the cofactor of activated protein C (representing about 40% of total Protein S) and b) C4-BP(C4 binding protein) bound protein S which exhibits no activity as cofactor of activated Protein C (representing about 60% of total PS). The objetive was to compare the PS dosage determination by two methods: immunoturbidimetric and clotting, and to investigate how pre-analytical variables affect the results. The following results were obtained: Clotting method: CV intra assay: (n=20): 4%, CV interassay (n=20, 3 days): 3.4%; immunoturbidimetric method CV intra assay (n=20): 3.7%: CV inter assay (n=20.3 days): 4.5%. There is a good correlation (R 2 = 0.94) between both methods; when the clotting method is calibrated in batch with the samples. There is significant difference between fresh and frozen ( -20 °C and -80 °C) samples when the latter have been desfrozen only once.
Registro:
| Documento: | Artículo |
| Título: | Influence of preanalytical variables in S protein determination |
| Autor: | Duboscq, C.; Kordich, L. |
| Filiación: | Servicio de Hematología Y Hemoterapia, Hospital Británico de Buenos Aires Programa de Evaluación Externa de Calidad, Fundación Bioquímica Argentina, Argentina Dpto. Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina Servicio de Hematología Y Hemoterapia, Hospital Británico de Buenos Aires, Perdriel 74, Ciudad Autónoma de BS. AS., Argentina |
| Palabras clave: | Free protein S; Prenalytical variables; Protein S |
| Año: | 2008 |
| Volumen: | 42 |
| Número: | 1 |
| Página de inicio: | 89 |
| Página de fin: | 93 |
| Título revista: | Acta Bioquimica Clinica Latinoamericana |
| Título revista abreviado: | Acta Bioquim. Clin. Latinoam. |
| ISSN: | 03252957 |
| CODEN: | ABCLD |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v42_n1_p89_Duboscq |
Referencias:
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Citas:
---------- APA ----------
Duboscq, C. & Kordich, L. (2008) . Influence of preanalytical variables in S protein determination . Acta Bioquimica Clinica Latinoamericana, 42(1), 89-93.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v42_n1_p89_Duboscq [ ]
---------- CHICAGO ----------
Duboscq, C., Kordich, L. "Influence of preanalytical variables in S protein determination " . Acta Bioquimica Clinica Latinoamericana 42, no. 1 (2008) : 89-93.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v42_n1_p89_Duboscq [ ]
---------- MLA ----------
Duboscq, C., Kordich, L. "Influence of preanalytical variables in S protein determination " . Acta Bioquimica Clinica Latinoamericana, vol. 42, no. 1, 2008, pp. 89-93.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v42_n1_p89_Duboscq [ ]
---------- VANCOUVER ----------
Duboscq, C., Kordich, L. Influence of preanalytical variables in S protein determination . Acta Bioquim. Clin. Latinoam. 2008;42(1):89-93.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v42_n1_p89_Duboscq [ ]
