Castañón, M.M.; Steyerthal, N.; Castagnino, J.M.; Garbossa, G. "Capillary Zone Electrophoresis study of the in vitro albumin glycation process " (2006) Acta Bioquimica Clinica Latinoamericana. 40(4):473-482
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Reducing sugars (such as glucose) can interact non-enzymatically with free amino-groups of proteins to form advanced glycation endproducts (AGEs). The objective of this work was to study the process of albumin glycation in vitro by Capillary Zone Electrophoresis (CZE). Bovine albumin (BSA; 50 mg/mL) and human serum (HS) were incubated with glucose (0.5 M) at 37°C for 12, 24 and 60 days. Aminoguanidine (AG) was used as inhibitor (0.5 M). Afterwards, HS-albumin was separated by precipitation and redissolved in phosphate-saline buffer. Fluorescent AGEs (λexcitation=338 nm, λ emission=442 nm) were detected after 12, 24 and 60 days of incubation. Fluorescence increased proportionally to the incubation times and AG was effective to inhibit the formation of fluorescent compounds. By CZE (boric acid 350 mM; pH 9,9; fused-silica gel capillary 50 μm x 50 cm; 25 kV; 90 μA; 20°C; λdetection=200 nm) longer migration times (MT) and wider peaks (PW-50) were observed, as the incubation time was prolonged. MT and PW-50 were partially reduced in the presence of AG. It can be concluded that CZE is a highly sensitive method to detect intermediate products during the protein glycation process. This procedure can be considered complementary to the fluorometric analysis used in AGEs' study.


Documento: Artículo
Título:Capillary Zone Electrophoresis study of the in vitro albumin glycation process
Autor:Castañón, M.M.; Steyerthal, N.; Castagnino, J.M.; Garbossa, G.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria, Int. Güiraldes 2160, C1428EHA Cd. Auton. de Buenos Aires, Argentina
Laboratorio de Análisis Clínicos de Alta Complejidad Dres, Castagnino. Sarmiento 69, 1870 Avellaneda. Buenos Aires, Argentina
Palabras clave:Advanced glycation endproducts; Aminoguanidine; Bovine serum albumin; Capillary electrophoresis; Fluorometric analysis; Human serum albumin; Bovinae
Página de inicio:473
Página de fin:482
Título revista:Acta Bioquimica Clinica Latinoamericana
Título revista abreviado:Acta Bioquim. Clin. Latinoam.


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---------- APA ----------
Castañón, M.M., Steyerthal, N., Castagnino, J.M. & Garbossa, G. (2006) . Capillary Zone Electrophoresis study of the in vitro albumin glycation process . Acta Bioquimica Clinica Latinoamericana, 40(4), 473-482.
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---------- CHICAGO ----------
Castañón, M.M., Steyerthal, N., Castagnino, J.M., Garbossa, G. "Capillary Zone Electrophoresis study of the in vitro albumin glycation process " . Acta Bioquimica Clinica Latinoamericana 40, no. 4 (2006) : 473-482.
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---------- MLA ----------
Castañón, M.M., Steyerthal, N., Castagnino, J.M., Garbossa, G. "Capillary Zone Electrophoresis study of the in vitro albumin glycation process " . Acta Bioquimica Clinica Latinoamericana, vol. 40, no. 4, 2006, pp. 473-482.
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---------- VANCOUVER ----------
Castañón, M.M., Steyerthal, N., Castagnino, J.M., Garbossa, G. Capillary Zone Electrophoresis study of the in vitro albumin glycation process . Acta Bioquim. Clin. Latinoam. 2006;40(4):473-482.
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