Artículo
Abstract:
Tobacco is one of the major preventable causes of morbidity and premature mortality in the world. A simple, rapid method for determination of cotinine, major metabolite of nicotine in urine, is described. The assay involves a fast liquid-liquid extraction with chloroform as a solvent in alkaline environment. The extract was dried at 37°C and the residue was dissolved in 100 μL of standard internal of pyridine in methanol solution, and an aliquot was analyzed by gas-liquid chromatograph equipped with a flame -ionization detector. This technique was performed to test urine of a sample of smokers and non-smokers who were previously asked about their smoking habit. The limit of detection was 3.1 ng/mL, and using two calibration curves, a linearity range from 3 a 5.000 ng/mL was covered. The median and quartiles (Q) of cotinine in smokers were 255.4 ng/mL, 15.9 ng/mL (Q1) - 1050.2 ng/mL (Q3) ng/mL and in non-smokers 15.8 ng/mL, 4.1 ng/mL (Q1) - 38.4 ng/mL (Q3). Wilcoxon sumrank: z = 4.865 p < 0.01. There was a relationship between cotinine concentration in urine and number of smoked cigarettes. Spearman's rho = 0.5672 (p < 0.001). This technique could identify smokers, second hand smokers and non-smokers using cut-off of 10-15 ng/mL of cotinine in urine, usually adopted in epidemiological studies.
Registro:
| Documento: | Artículo |
| Título: | Cotinine detection and tobacco smoke exposure |
| Autor: | Vacchino, M.N.; Velurtas, S.M.; Salinas, G.P.; Garcialoredo, H.H. |
| Filiación: | Departamento de Química, Facultad de Ciencias Exactas Y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina Departamento de Investigación, Instituto Nacional de Epidemiología Dr. Juan H. Jara, Mar del Plata, Argentina Laboratorio BAS, Mar del Plata, Argentina Instituto Nacional de Epidemiología Dr. Juan H. Jara, Mar del Plata, Argentina Charlone 172, (7600) Mar del Plata, Buenos Aires, Argentina |
| Idioma: | Español |
| Palabras clave: | Cotinine; Environmental tobacco smoke; Epidemiological studies; Second hand smoker; Nicotiana tabacum |
| Año: | 2006 |
| Volumen: | 40 |
| Número: | 2 |
| Página de inicio: | 181 |
| Página de fin: | 185 |
| Título revista: | Acta Bioquimica Clinica Latinoamericana |
| Título revista abreviado: | Acta Bioquim. Clin. Latinoam. |
| ISSN: | 03252957 |
| CODEN: | ABCLD |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino |
Referencias:
- (2000) La Epidemia del Tabaco, pp. 23-31. , Organización Panamericana de la Salud. Publicación Científica No 577. Washington, DC 20037, EUA
- Benowitz, N., Cotinine as a biomarker of environmental tobacco smoke exposure (1996) Epidemiol Rev, 18 (2), pp. 188-203
- Bernert Jr., J.T., Turner, W.E., Pirkle, J.L., Sosnoff, C.S., Akins, J.R., Waldrep, M.K., Development and validation of sensitive method for determination of serum cotinine in smokers and nonsmokers by liquid chromatography/atmospheric pressure ionization tandem mass spectrometry (1997) Clin Chem, 43, pp. 2281-2291. , a
- Dhar, P., Measuring tobacco smoke exposure: Quantifying nicotine/cotinine concentration in biological samples by colorimetry, chromatography and immunoassay methods (2004) J Pharm Biomed Anal, 35 (1), pp. 155-168
- Bazylak, G., Brózik, H., Sabanty, W., HPTLC screening assay for urinary cotinine as biomarker of environmental tobacco smoke exposure among male adolescents (2000) J Pharm Biomed Anal, 24 (1), pp. 113-123
- Kuo, H.W., Yang, J.S., Chiu, M.C., Determination of urinary and salivary cotinine using gas and liquid chromatography and enzyme-linked immunosorbent assay (2002) J Chromatogr B Biomed Appl, 768 (2), pp. 297-303
- Skarping, G., Willers, S., Dalene, M., Determination of cotinine in urine using glass capillary gas chromatography and selective detection, with special reference to the biological monitoring of passive smoking (1988) J Chromatogr, 454, pp. 293-301
- (2005) Third National Report on Human Exposure to Environmental Chemicals, , http://www.cdc.gov/exposurereport/3rd/pdf/thirdreport.pdf, Centers for Disease Control and Prevention. Available from: URL (01/12/2005)
- Bono, R., Russo, R., Scursatone, E., Gilli, G., Involuntary exposure to tobacco smoke in adolescents: Urinary cotinine and environmental factors (1996) Arch Environ Health, 51 (2), pp. 127-131
Citas:
---------- APA ----------
Vacchino, M.N., Velurtas, S.M., Salinas, G.P. & Garcialoredo, H.H. (2006) . Cotinine detection and tobacco smoke exposure . Acta Bioquimica Clinica Latinoamericana, 40(2), 181-185.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino [ ]
---------- CHICAGO ----------
Vacchino, M.N., Velurtas, S.M., Salinas, G.P., Garcialoredo, H.H. "Cotinine detection and tobacco smoke exposure " . Acta Bioquimica Clinica Latinoamericana 40, no. 2 (2006) : 181-185.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino [ ]
---------- MLA ----------
Vacchino, M.N., Velurtas, S.M., Salinas, G.P., Garcialoredo, H.H. "Cotinine detection and tobacco smoke exposure " . Acta Bioquimica Clinica Latinoamericana, vol. 40, no. 2, 2006, pp. 181-185.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino [ ]
---------- VANCOUVER ----------
Vacchino, M.N., Velurtas, S.M., Salinas, G.P., Garcialoredo, H.H. Cotinine detection and tobacco smoke exposure . Acta Bioquim. Clin. Latinoam. 2006;40(2):181-185.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v40_n2_p181_Vacchino [ ]
