Abstract:
Heparin is mainly found in mastocytes, and during many decades the studies of the coagulation mechanism were focused on this heteropolysaccaride. Dermatan sulfate has a more abundant distribution on tissues and on the blood vascular system. On the other hand, the anticoagulant activity of the second cofactor of heparin is stimulated by dermatan sulfate, but using ten times less concentration than heparin. Our earlier experiments with heparin and Concanavalin A, and heparin and the first protein of the complement system, C1, have shown the isolation in the heparin molecule by precipitation with both proteins of fractions with very high anticoagulant activity. In both cases the use of very low ionic strength and the presence of calcium ions were necesary. We have extended those experiments to the interaction with dermatan sulfate, and we have also obtained a fraction of dermatan sulfate in the precipitate with very low molecular weight, and with four times higher anticoagulant activity than the fraction that remains in the supernatant. The sulfate content of this fraction is three times higher, as well as its capacity for thrombous inhibition. Molecular weight of this fraction is 8000 Daltons.
Registro:
Documento: |
Artículo
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Título: | Isolation of a small fraction of dermatan sulfate with potential therapeutical application |
Autor: | Recondo, E.F.; Calabrese, G.C.; Alberto, M.F.; Lazzari, M.; Del Valle Fernández De Recondo, M.E. |
Filiación: | Bioquímica Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Ciudad Universitaria de Núñez, (1428) Cd. Auton. de Buenos Aires, Argentina Cátedra de Biologia Celular, Facultad de Farmacia Y Bioquímica, Universidad de Buenos Aires, Junin 954, (1113) Cd. Auton. de Buenos Aires, Argentina Departamento de Hemostasia Y Trombosis, Academia Nacional de Medicina, Cd. Autónoma de Buenos Aires, Argentina Juncal 2186, 1125 Cd. Auton. de Buenos Aires, Argentina
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Palabras clave: | Calcium ions; Dermatán sulfate; First component of the complement system; Heparin cofactor II; High sulfate content; Low ionic strength; Low molecular weight; Molecular interactions; Thrombous dissolution |
Año: | 2005
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Volumen: | 39
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Número: | 2
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Página de inicio: | 163
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Página de fin: | 169
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Título revista: | Acta Bioquimica Clinica Latinoamericana
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Título revista abreviado: | Acta Bioquim. Clin. Latinoam.
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ISSN: | 03252957
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CODEN: | ABCLD
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n2_p163_Recondo |
Referencias:
- Dahlback, B., Blood coagulation (2000) Lancet, 355, pp. 1627-1632
- Tollefsen, D., Majerus, D.W., Blank, M.K., Heparin Cofactor II. Purification and properties of a heparin-dependent inhibitor of thrombin in human plasma (1982) J Biol Hem, 257, pp. 2162-2169
- Tollefsen, D., Insight into the mechanism of action of heparin cofactor II (1995) Thromb Hem, 74 (5), pp. 1209-1214
- Bedayan, P., Boccalon, H., Dupouy, D., Boneu, B., Dermatan sulfate is a more potent inhibitor of clot-bound thrombin than unfractionated and low molecular weight heparins (1994) Thromb and Haem, 71 (5), pp. 576-580
- Tollefsen, D.M., Pestka, C.A., Monafo, W., Activation heparin cofactor II by dermatan sulfate (1983) J Biol Chem, 258 (11), pp. 6713-6716
- Rosenberg, R.D., Armand, G., Lam, L., Structure-function relationships of heparin species (1978) Proc Natl Acad Sci, 75 (7), pp. 3065-3069. , USA
- Lindahl, U., Backstrom, G., Hook, M., Thunberg, L., Fransson, L.A., Linker, A., Structure of antithrombin-binding site in heparin (1979) Proc Natl Acad Sci, 76 (7), pp. 3198-3202. , USA
- Maimone, M., Tollefsen, D.M., Structure of a dermatan sulfate hexasaccharide that binds to heparin cofactor II with high affinity (1990) J Biol Chem, 265 (39), pp. 18263-18271
- Tollefsen, D.M., Peacock, M.E., Monafo, W.J., Molecular size of Dermatan Sulfate oligosaccharides required to bind an activate Heparin Cofactor II (1986) J Biol Chem, (261), pp. 8854-8858
- Fernández De Recondo, M.E., Legorburu, C., Monge, J.C., Recondo, E.F., Heparin and Concanavalin A interaction. Isolation of fraction with higher anticoagulant activity (1988) Biochem Biophys Res Commun, 155 (1), pp. 216-223
- Monge, J.C., Recondo, E.F., Fernández De Recondo, M.E., Heparin and Concanavalin A interaction as a model for studying the mechanism of the anticoagulant activity (1989) Thromb Res, 54, pp. 237-243
- Monge, J.C., Legorburu, C., Fernández De Recondo, M.E., Recondo, E.F., Heparin and Concanavalin A interaction: A useful model for studying the mechanism of anticoagulant activity (1989) Ann New York Acad Sc, 556, pp. 462-464
- Fernández De Recondo, M.E., Monge, J.C., Recondo, E.F., Interaction of Concanavalin A with a fraction of dermatan sulfate containing a very high anticoagulant activity (1995) Cell Mol Biol, 41 (4), pp. 465-472
- Cruse, J.M., Lewis Jr., R.E., Complement Today. Complement Profiles. Basel, Karger, 1993. Heparin Is Not Just An Anticoagulant Anymore: Six and One-Half Decades of Studies on the Ability of Heparin to Regulate Complement Activity, 1, pp. 96-120. , R. Erik Edens, Robert J. Linhardt, John M. Weiler
- Calabrese, G.C., Recondo, M.M., Fernández De Recondo, M.E., Recondo, E.F., The first component of the human complement system recognizes the active fraction of heparin (1997) Cell and Mol Biol, 43 (2), pp. 237-242
- Calabrese, G.C., Leocata Nieto, F., Recondo, E.F., Fernández De Recondo, M.E., An active fraction of unfractionated heparin from a natural source is recognized by the first component of the complement system (2001) Cell and Mol Biol, 47 (17), pp. 127-131
- Calabrese, G.C., Recondo, E.F., Fernández De Recondo, M.E., Antithrombin and first protein C1 recognize the same active heparin fraction (2002) Thromb Res, 105, pp. 537-541
- Almeda, S., Rosenberg, R.D., Bing, D.H., The binding properties of human complement component C1q; interaction with mucopolysaccharides (1983) J Biol Chem, 258, pp. 785-791
- Calabrese, G.C., Alberto, M.F., Tubio, R., Marani, M., Fernández De Recondo, M.E., Lazzari, M., A small fraction of dermatan sulfate with significantly increased anticoagulant activity was selected by interaction with the first complement protein (2004) Thromb Res, 113, pp. 243-250
- Imbimbo, B.P., Sié, P., Agnelli, G., Saivin, S., Dupouy, D., Damiani, M., Intramuscular dermatan sulfate MF701 in patients with hip fracture: Relationship between pharmacokinetics and antithrombotic efficacy (1994) Thromb Haem, 71 (5), pp. 553-557
- Pavao, M., Aiello, K.R., Werneck, C., Silva, L., Valente, A.P., Mulloy, B., Highly sulfated dermatan sulfate from ascidians. Structure versus anticoagulant activity of these glycosaminoglycans (1998) J Biol Chem, 273 (43), pp. 27848-27857
- Mascellani, G., Liverani, L., Parma, B., Bergonzini, G., Bianchini, P., Active site for heparin cofactor II in low molecular mass dermatan sulfate. Contribution to the antithrombotic activity of fractions with high affinity for heparin cofactor II (1996) Throm Res, 84 (1), pp. 21-32
- Bing, D., Purification of the first component of human complement by affinity chromatography on human gamma globulin linked to sepharose (1971) J Immunol, 107 (5), pp. 1243-1249
- Twlikki, T., Terho, T., Hartiala, K., Method for determination of the sulfate content of glycosaminoglycans (1971) Anal Biochem, 41, pp. 471-476
- Doutremepuich, C., Azougagh Oualane, F., Doutremepuich, F., Fareed, J., New class of heparin derivates with a potent antithrombotic effect and a very limited hemorrhagic activity (1996) Thromb Res, 83 (3), pp. 217-228
- Fareed, J., Hoppensteadt, D., Bick, R., An update on heparins at the beginning of the new millennium (2000) Sem Thromb Haem, 26 (1), pp. 5-21
- Bedayan, P., Boccalon, H., Dupouy, D., Boneu, B., Dermatan sulfate is a more potent inhibitor of clot-bound thrombin than unfractionated and low molecular weight heparins (1994) Thromb Haem, 71 (5), pp. 576-580
- Griffith, M.J., Marbet, G.A., Dermatan sulfate and heparin can be fractionated by affinity for heparin cofactor II (1983) Biochem Biophys Res Commun, (112), pp. 663-670
Citas:
---------- APA ----------
Recondo, E.F., Calabrese, G.C., Alberto, M.F., Lazzari, M. & Del Valle Fernández De Recondo, M.E.
(2005)
. Isolation of a small fraction of dermatan sulfate with potential therapeutical application . Acta Bioquimica Clinica Latinoamericana, 39(2), 163-169.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n2_p163_Recondo [ ]
---------- CHICAGO ----------
Recondo, E.F., Calabrese, G.C., Alberto, M.F., Lazzari, M., Del Valle Fernández De Recondo, M.E.
"Isolation of a small fraction of dermatan sulfate with potential therapeutical application "
. Acta Bioquimica Clinica Latinoamericana 39, no. 2
(2005) : 163-169.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n2_p163_Recondo [ ]
---------- MLA ----------
Recondo, E.F., Calabrese, G.C., Alberto, M.F., Lazzari, M., Del Valle Fernández De Recondo, M.E.
"Isolation of a small fraction of dermatan sulfate with potential therapeutical application "
. Acta Bioquimica Clinica Latinoamericana, vol. 39, no. 2, 2005, pp. 163-169.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n2_p163_Recondo [ ]
---------- VANCOUVER ----------
Recondo, E.F., Calabrese, G.C., Alberto, M.F., Lazzari, M., Del Valle Fernández De Recondo, M.E. Isolation of a small fraction of dermatan sulfate with potential therapeutical application . Acta Bioquim. Clin. Latinoam. 2005;39(2):163-169.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n2_p163_Recondo [ ]