Artículo
Duboscq, C.; Kordich, L. "Effect of sodium citrate concentration on coagulation assays " (2005) Acta Bioquimica Clinica Latinoamericana. 39(1):87-92
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Abstract:
The aim of this study was to assess the extent of influence of citrate concentration on the results of routine coagulation assays (prothrombin time (PT) and activated partial thromboplastin time (APTT). PT was evaluated using three tromboplastins of different origin and APTT was determined by four different APTT reagents. Three methods of clot detecction were used (manual, photo-optic and electromecanic). Three groups were studied: healthy volunteers, outpatients receiving stable dosis of oral anticoagulant therapy and hospitalized patients receiving non fractionated heparin therapy. There were no statistically significant differences of TP expressed in percentaje. In patients receiving dicumarinic therapy, the INR (International Normatized Ratio) obtained with two thromboplastins of lower ISI showed a statistically significant difference between two citrate concentrations. This difference is more important when the RIN is greater than 3.5. Effects of sodium citrate concentration on APTT were satistically significant in all groups when a reagent sensitive to aid the detection of lupus anticoagulant was used. For APTT the greatest change was observed in patients receiving heparin therapy.
Registro:
| Documento: | Artículo |
| Título: | Effect of sodium citrate concentration on coagulation assays |
| Autor: | Duboscq, C.; Kordich, L. |
| Filiación: | Universidad de Buenos Aires, Servicio de Hematología Hospital Británico de Buenos Aires, Coordinadora del Subprograma Hemostasia del PEEC, Argentina Universidad de Buenos Aires, Facultad de Ciencias Exactas Y Naturales, UBA Coordinadora del Subprograma Hemostasia del PEEC, Argentina Hospital Británico, Servicio de Hematología, Perdriel 74, 1280 Buenos Aires, Argentina |
| Palabras clave: | Activated partial thromboplastin time; Prothrombin time; Sodium citrate concentration; citrate sodium; heparin; reagent; thromboplastin; anticoagulant therapy; article; blood clotting; concentration (parameters); controlled study; dose response; heparinization; human; international normalized ratio; normal human; outpatient; partial thromboplastin time; prothrombin time; sensitivity analysis; statistical significance |
| Año: | 2005 |
| Volumen: | 39 |
| Número: | 1 |
| Página de inicio: | 87 |
| Página de fin: | 92 |
| Título revista: | Acta Bioquimica Clinica Latinoamericana |
| Título revista abreviado: | Acta Bioquim. Clin. Latinoam. |
| ISSN: | 03252957 |
| CODEN: | ABCLD |
| CAS: | citrate sodium, 18996-35-5, 994-36-5; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; thromboplastin, 9035-58-9 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n1_p87_Duboscq |
Referencias:
- Duboscq, C., Castañon, M., Control de calidad en el laboratorio de Hemostasia (2003) Fundamentos Prácticos en El Laboratorio de Hemostasia, pp. 47-56. , Grupo Argentino de Hemostasia 1 a ed., La Plata
- (1998) Collection, Transport and Processing of Blood Specimens for Coagulation Testing and Performance of Coagulation Assays. 2 a Ed. (Approved Guideline) NCCLS Documents H21-A3, , (ISBN 1-56238-363-9), 1998. NCCLS, 940 West Vallery Road, Wayne, Pensylvania. 19087, USA
- Adcok, D., Kressin, D., Marlar, R., Effect of 3,2% sodium citrate concentration on routine coagulation testing (1997) Am J Clin Pathol, 107, pp. 105-110
- Chantarangkul, V., Tripodi, A., Clerici, M., Negri, B., Manucci, P., Assesment of the influence of citrate concentration on the International Normalized Ratio (INR) determined with twelve reagent instrument combinations (1998) Thromb Haemost, 80, pp. 258-262
- Van Den Besselaar, A.M., Chantarangkul, V., Tripodi, A., A comparison of two sodium citrate concentration in two evacuated blood collection systems for prothrombin time and ISI determination (2000) Thromb Haemost, 84, pp. 664-667
- Duncan, E., Casey, C., Duncan, B., Lloyd, J., Effect of concentration of trisodium citrate anticoagulant on calculation of the international normalised ratio and the international sensitivity index of thromboplastin (1994) Thromn and Haemost, 72 (1), pp. 84-88
- Guidelines for thromboplastin and plasma used to control oral anticoagulant therapy (1999) WHO Technical Report Series No 889, pp. 64-93. , WHO Expert Committee on Biological Standarization. Forty-Eighth Report. Anex 3. Geneva: World Health Organization
- Quick, A., The prothrombin in hemophilia and in obstructive jaundice (1935) J Biol Chem, 109, pp. 73-74
- Poller, L., International Normalized Ratios (RIN): The first 20 years (2004) J Thromb Haemost, 2, pp. 849-860
- Naghibi, F., Yangsook, H., Dodds, W., Lawrence, C., Effects of Reagent and Instrument on Prothrombin Times, Activated Partial Thromboplastin Times and Patients/control Ratios
- Bjornsson, D., Nash, V.P., Variability in heparin sensitivity of APTT reagents (1986) Am J Clin Pathol, 86, pp. 199-204
- Lotin, L., Woodhams, B.J., Saureau, C., Robert, A., Aillaud, M.F., Arnaud, E., The clinical relevance of the citrate effect on international normalized ratio determinations depends on the reagent and instrument combination used (2001) Blood Coagulation and Fibrinolysis, 12, pp. 399-404
- Loeliger, E.A., ICSH/ICTH recomendations for reporting prothrombin time in oral anticoagulant control (1985) Thromb Haemost, 54, pp. 155-166
Citas:
---------- APA ----------
Duboscq, C. & Kordich, L. (2005) . Effect of sodium citrate concentration on coagulation assays . Acta Bioquimica Clinica Latinoamericana, 39(1), 87-92.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n1_p87_Duboscq [ ]
---------- CHICAGO ----------
Duboscq, C., Kordich, L. "Effect of sodium citrate concentration on coagulation assays " . Acta Bioquimica Clinica Latinoamericana 39, no. 1 (2005) : 87-92.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n1_p87_Duboscq [ ]
---------- MLA ----------
Duboscq, C., Kordich, L. "Effect of sodium citrate concentration on coagulation assays " . Acta Bioquimica Clinica Latinoamericana, vol. 39, no. 1, 2005, pp. 87-92.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n1_p87_Duboscq [ ]
---------- VANCOUVER ----------
Duboscq, C., Kordich, L. Effect of sodium citrate concentration on coagulation assays . Acta Bioquim. Clin. Latinoam. 2005;39(1):87-92.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v39_n1_p87_Duboscq [ ]
