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The aim of this study was to assess the extent of influence of citrate concentration on the results of routine coagulation assays (prothrombin time (PT) and activated partial thromboplastin time (APTT). PT was evaluated using three tromboplastins of different origin and APTT was determined by four different APTT reagents. Three methods of clot detecction were used (manual, photo-optic and electromecanic). Three groups were studied: healthy volunteers, outpatients receiving stable dosis of oral anticoagulant therapy and hospitalized patients receiving non fractionated heparin therapy. There were no statistically significant differences of TP expressed in percentaje. In patients receiving dicumarinic therapy, the INR (International Normatized Ratio) obtained with two thromboplastins of lower ISI showed a statistically significant difference between two citrate concentrations. This difference is more important when the RIN is greater than 3.5. Effects of sodium citrate concentration on APTT were satistically significant in all groups when a reagent sensitive to aid the detection of lupus anticoagulant was used. For APTT the greatest change was observed in patients receiving heparin therapy.


Documento: Artículo
Título:Effect of sodium citrate concentration on coagulation assays
Autor:Duboscq, C.; Kordich, L.
Filiación:Universidad de Buenos Aires, Servicio de Hematología Hospital Británico de Buenos Aires, Coordinadora del Subprograma Hemostasia del PEEC, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas Y Naturales, UBA Coordinadora del Subprograma Hemostasia del PEEC, Argentina
Hospital Británico, Servicio de Hematología, Perdriel 74, 1280 Buenos Aires, Argentina
Palabras clave:Activated partial thromboplastin time; Prothrombin time; Sodium citrate concentration; citrate sodium; heparin; reagent; thromboplastin; anticoagulant therapy; article; blood clotting; concentration (parameters); controlled study; dose response; heparinization; human; international normalized ratio; normal human; outpatient; partial thromboplastin time; prothrombin time; sensitivity analysis; statistical significance
Página de inicio:87
Página de fin:92
Título revista:Acta Bioquimica Clinica Latinoamericana
Título revista abreviado:Acta Bioquim. Clin. Latinoam.
CAS:citrate sodium, 18996-35-5, 994-36-5; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; thromboplastin, 9035-58-9


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---------- APA ----------
Duboscq, C. & Kordich, L. (2005) . Effect of sodium citrate concentration on coagulation assays . Acta Bioquimica Clinica Latinoamericana, 39(1), 87-92.
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---------- CHICAGO ----------
Duboscq, C., Kordich, L. "Effect of sodium citrate concentration on coagulation assays " . Acta Bioquimica Clinica Latinoamericana 39, no. 1 (2005) : 87-92.
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---------- MLA ----------
Duboscq, C., Kordich, L. "Effect of sodium citrate concentration on coagulation assays " . Acta Bioquimica Clinica Latinoamericana, vol. 39, no. 1, 2005, pp. 87-92.
Recuperado de [ ]
---------- VANCOUVER ----------
Duboscq, C., Kordich, L. Effect of sodium citrate concentration on coagulation assays . Acta Bioquim. Clin. Latinoam. 2005;39(1):87-92.
Available from: [ ]