Artículo

Rossi, E.B.; Vizcargüenaga, M.I. "Activación plaquetaria por trombina" (1999) Acta Bioquimica Clinica Latinoamericana. 33(1):35-47
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Abstract:

When an agonist binds to a specific receptor on the platelet membrane a series of morphological and metabolic changes begin which induces the shape change, aggregation and granule release, thrombin multifunctional serinoprotease and a strong platelet agonist, has two different types of binding sites on platelets membranes: high and moderate affinity. The last one belongs to the β2 adrenergic receptors with the seven transmembrane domains coupled to specific G proteins, which triggers the metabolic pathways with specific enzymes. Phospholipase Cβ activity forms two seconds messengers: Inositol 3 phosphate that induces calcio release from dense tubular system to the citosol, and the diacylglicerol which actives protein quinase C. The platelets do not proliferate but it have been found in the enzymes related to oncogenes. Tyrosine kinase family, related to cellular proliferation and oncogenes, phosphorilate tyrosine residues belong mostly to the citosolic non receptor group, as Scr, Syk and FAK. Pi P2 hydrolysis results also from the stimulation of PLCγ which is independent of G proteins. Thrombin induces the formation of the trimeric complex consisting in phospholypase Cγ, Ras GAP and Rap Ib, bringing phospholypase Cγ in contact with membrane phosphoinositides. Phosphoinositol 3 kinase catalysates phosphorilation of 3 inositol phospholipids on the 3 position of the inositol ring forming new phosphoinositol products. The identification of thrombin receptors in platelets give us the opportunity to contemplate the development of a new class of agents that will selectively inhibit the effects of thrombin on cells.

Registro:

Documento: Artículo
Título:Activación plaquetaria por trombina
Autor:Rossi, E.B.; Vizcargüenaga, M.I.
Filiación:Lab. de Hemostasia y Trombosis, Dpto. Quim. Biológica, Ciudad Universitaria, CP 1428, Buenos Aires, Argentina
Laboratorio de Hemostasia, Sanatorio Guemes S.A. Cordoba 3933, Buenos Aires, Argentina
Palabras clave:Haemostasis; Platelet aggregation; Thrombin; Thrombin receptor
Año:1999
Volumen:33
Número:1
Página de inicio:35
Página de fin:47
Título revista:Acta Bioquimica Clinica Latinoamericana
Título revista abreviado:Acta Bioquim. Clin. Latinoam.
ISSN:03252957
CODEN:ABCLD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v33_n1_p35_Rossi

Referencias:

  • Hawiger, J., Mechanisms involved in platelet vessel wall interaction (1995) Thromb. Haemost., 74, pp. 369-372
  • Glusa, E., Vascular effects of thrombin (1992) Semin. Thromb. Haemost., 18, pp. 296-304
  • Guillin, M.C., Bezeaud, A., Bouton, M.C., Jandrot-Perrus, M., Thrombin Specificity (1995) Thromb. Haemost., 74, pp. 129-133
  • Bode, W., Stubbs, M.T., Spatial structure of thrombin as a guide to its multiple sites of interaction (1993) Seminars in Trhomb. Hemost., 19, pp. 321-334
  • Jamieson, G.A., Pathophysiology of platelet thrombin receptors (1997) Thromb. Haemost., 78, pp. 242-246
  • Woolkalis, M.J., Mannig, D.R., Brass, L.F., Opposite effects of thrombin on cAMP formation in human platelets and endotelial cells (1990) J. Cell Biol., 111, pp. 215a
  • Brass, L.F., Woolkalis, M.J., Dual regulation of cAMP formation by thrombin in HEL cell, a leukemic cell line with megakaryocytic properties (1992) Biochem. J., 281, pp. 73-80
  • Ganguli, P., Binding of Thrombin to functionally defective platelets: A hypothesis of the nature of the thrombin receptor (1997) J. Biol. Hematol., 7, pp. 47-51
  • Jamieson, G.A., Okamura, T., Reduced thrombin-binding and aggregation in Bernard Soullier platelets (1978) J. Clin. Invest., 1, pp. 861-864
  • Jandrot-Perrus, M., Rendu, F., Caen, J.P., Levy-Toledano, S., Guillin, M.C., The common pathway for alpha and gamma thrombin induced platelet activation is independent of GPIb: A study of Bernard-Soulier platelet (1990) Br. J. Haematol., 5, pp. 385-392
  • De Marco, L., Mazzucato, M., Masotti, A., Fenton, J.W., Jandrot-Perrus, M., Bouton, M.C., Lanza, F., Guillin, M.C., Thrombin Interaction with platelet membrane glycoprotein Ib (1996) Semin. Thromb. Haemost., 2, pp. 151-156
  • Jandrot-Perrus, M., Didry, D., Guillin, M.C., Nurden, A., Cross linking of alpha and gamma thrombin to distinct binding sites on human platelets (1998) Eur. J. Biochem., 174, pp. 359-367
  • Yamamoto, N., Greco, N.J., Barnard, M.R., Tanke, K., Yamazaki, H., Jamieson, G.A., Michelson, A.D., Glycoprotein Ib (GPIb)-dependent and GPIb-independent pathways of thrombin induces platelet activation (1991) Blood, 7, pp. 1740-1748
  • Vu, T.R., Hung, K.T., Wheaton, V., Coughin, S., Molecular clonning of a functional Thrombin Receptor Activation (1991) Cell, 64, pp. 1057-1068
  • Steven, M., Seiler, Thrombin Receptor Antagonists (1996) Semin. Thromb. Haemost., 22, pp. 223-232
  • Zhong, C., Hayzer, D.J., Corson, M.A., Runge, M.S., Molecular Clonning of the rat vascular smooth muscle Thrombin Receptor: Evidence for in vivo regulation by basic fibroblast growth factor (1992) J. Biol. Chem., 267, pp. 16975-16979
  • Glembostki, C.C., Irons, C.E., Krown, K.A., Murray, S.F., Sprenkle, A.B., Sei, C.A., Myocardial alpha-thrombin receptor activation induces hypertrophy and increases trial natriutic factor gene expression (1993) J. Biol. Chem., 268, pp. 20646-20652
  • Brass, L.F., Wookalin, M.J., Manning, D.R., Interactions in platelet between G proteins and the agonist that stimulate phospholipase C and inhibit Adenyl cyclase (1988) J. Biol. Chem., 263, pp. 5348-5355
  • Vu, T., Wheaton, V., Hung, D.T., Charo, I., Coughlin, S.R., Domains specifying Thrombin-receptor interaction (1991) Nature, 363, pp. 674-677
  • Liu, L.W., Vu, T., Esmon, C.T., Coughlin, S.R., The region of the thrombin receptor resembling hirudin binds to thrombin and alters enzyme specificity (1991) J. Biol. Chem., 266, pp. 16977-16980
  • Mathews, I., Padmanabhan, K.P., Ganesh, V., Tulinsky, A., Ishii, M., Chen, J., Turck, C.W., Fenton, J.W., Crystalographic structures of Thrombin complexed with thrombin receptor peptides: Existence of expected and novel binding modes (1994) Biochemistry, 33, pp. 3266-3279
  • Kinlough-Rathbone, R.L., Rand, M.L., Packham, M.A., Rabbit and rat platelets do not respond to thrombin receptor peptides that active human platelets (1993) Blood, 82, pp. 103-106
  • Vouret-Craviari, V., Van Obberghem-Schlling, E., Scimeca, J.C., Van Obbenghen, E., Pouyssegur, J., Differential activation of p44mak (ERK1) by a-thrombin and thrombin receptor peptide agonist (1993) Biochem. J., 289, pp. 209-214
  • Lau, L.F., Pumiglia, K., Coté, Y.P., Feinstein, M.B., Thrombin receptor agonist peptides, in contrast to thrombin itself, are not full agonist for activation and signal transduction in human platelets in the absence of platelet-derived secondary mediators (1994) Biochem. J., 303, pp. 391-400
  • Nanevicz, T., Ishii, M., Wang, L., Chen, M., Chen, J.I., Turck, C.W., Cohen, F.E., Coughlin, S.R., Mechanisms of thrombin receptor agonist specifity: Chimeric receptor and complementary mutations identify an agonist recognition site (1995) J. Biol. Chem., 270, pp. 21619-21625
  • Baffy, G., Yang, L., Ray, S., Manning, D.R., Williamson, J.R., G protein coupling to the thrombin receptor in Chinese hamster lung fibroblast (1994) J. Biol. Chem., 269, pp. 8483-8487
  • Field, T., Casey, P., Signaling functions and biochemical properties of pertussi toxin-resistant G proteins (1997) Biochem. J., 321, pp. 561-571
  • Brass, L., Manning, D., Cichowski, K., Abrams, C., Signaling through G protein in platelets: To the integrins and beyond (1997) Thromb. Haemost., 78, pp. 581-589
  • Hung, D.T., Wong, Y.H., Vu, T., Coughlin, S.R., The cloned platelet thrombin receptor couples to at least two distinct effectors to stimulate phosphoinositide hydrolysis and inhibit adenylyl cycase (1992) J. Biol. Chem., 267, pp. 20381-20834
  • Nystedt, S., Emilsson, K., Wahlestedt, C., Sundelin, J., Molecular cloning of a potential proteinase activated receptor (1994) Proc. Natl. Acad. Sci. USA, 91, pp. 9208-9212
  • Cerletti, C., Evangelista, V., Molino, M., De Gaetano, G., Platelet activation by polymorphonuclear leukocytes: Rol of cathepsin G and P-selectin (1995) Thromb. Haemost., 74, pp. 218-223
  • Ishii, K., Chen, J., Ishii, M., Koch, W.J., Freddman, N.J., Lefkowitz, R.J., Coughlin, S.R., Inhibition of thrombin receptor signaling by a G-protein coupled receptor Kinase. Functional specific-ity among G-protein coupled receptor kinases (1994) J. Biol. Chem., 269, pp. 1125-1130
  • Brass, L.F., Pizarro, S., Ahuja, M., Belmonte, E., Stadel, J., Hoxie, J.A., Changes in structure and function of the human thrombin receptor during activation, internalization and recycling (1994) J. Biol. Chem., 269, pp. 2943-2952
  • Brass, L.F., Homologous desensitization of HEL cell thrombin receptors distinguishable roles for proteolysis and phosforylation (1992) J. Biol. Chem., 267, pp. 6044-6050
  • Norton, K.J., Scarborough, R.M., Kutok, J.L., Escobedo, M., Nannizzi, L., Coller, B.S., Immunologic analysis of the cloned platelet thrombin receptor activation mechanisms: Evidence supporting receptor clevage, release of the N-terminal peptido, and insertion of the tethered ligand into a protected environment (1993) Blood, 82, pp. 2125-2136
  • Rhee, S.G., Inositol phospholipid specific phospholipase C: Interaction of the γ1 isoform with tyrosine kinase (1991) Trends Biochem. Sci., 16, pp. 2976-3301
  • Majerus, P.W., Connolly, T.M., Bans, V.S., Inhorn, R.C., Ross, T.S., Lips, D.L., Inositol phosphates: Synthesis and degradation (1988) J. Biol. Chem., 263, pp. 3051-3058
  • Lapetina, E.G., Inositol phospholipids and GTP-binding proteins in signal transduction in stimulated human platelets (1986) Adv. Prostaglandin Thromboxane Leucotriene Res., 16, pp. 217-222
  • Hoffman, R., Benz, E., Shattil, E., (1991) Hematologic Basic Principles and Practices, , Ed. Stanford, USA
  • Levy-Toledano, S., Mac Lovf, V.I., Chen, F., Messagers bioquimique plaquetarie (1985) Nouj. Rev. Fr. Hematol., 4, pp. 203-207
  • Yamamoto, K., Lapetina, E., Protein kinase C mediated formation of phosphatidylinositol 3,4 bisphosphate in human platelets (1990) Biochem. Biophys. Res. Commun., 168, pp. 466-472
  • Lee, G.R., Bithell, T.C., Foester, J., Athens, J., Lukens, J., (1992) Wintrobe's Clinical Hematology, , De Lea and Febiger, Londres
  • Torti, M., Lapetina, E., Role of Rap 1b and p21 Ras GTPase activating protein in the regulation of PLCγ in human platelets (1992) Proc. Natl. Acad. Sci. USA, 89, pp. 7796-7800
  • Toker, A., Meyer, M., Reddy, K., Falck, J.R., Aneja, R., Aneja, S., Parra, A., Cantley, L.C., Activation of protein kinase C famly members by the novel polyphosphoinositides Ptd Ins 3,4-P2 and In5 3.4.5P3 (1994) J. Biol. Chem., 269, pp. 22538-32367
  • Gironcel, D., Racan-Sultan, C., Payrastre, B., Haricot, M., Borchet, G., Kieffer, N., Breton, M., Chap, H., αIIbβ3 integrin mediates adhesion of human platelets to a fibrinogen matrix triggers phospholipase C activation and phosphatidylinositol 3'-4'-biphosphato accumulation (1996) Febs Letters, 386, pp. 253-256
  • Guinebault, C., Payrastre, B., Sultran, C., Manco, G., Levy-Toledano, S., Tyrosine kinases and phosphoinositide metabolism in thrombin stimulated human platelets (1993) J. Biol. Chem., 292, pp. 851-856
  • Jackson, S., Schoenwaelder, J., Yvan, Y., Salem, A., Cooray, P., Non receptor protein tyrosine kinases and phosphatases in human platelets (1996) Thromb. Haemost., 76, pp. 640-650
  • Avraham, S., London, R., Fu, Y., Ota, S., Hiregowdara, D., Li, J., Jiang, S., Groopman, J.E., Identification and characterization of a novel related adhesion focal tyrosine kinase (RAFTK) from megakaryocytes and brain (1995) J. Biol. Chem., 270, pp. 27742-27751
  • Levy-Toledano, S., Grelar, F., Caen, V., Macclof, V., KRDS, a peptide derivates from human lactotransferrin, inhibits thrombin induce thromboxane synthesis by a cycloxygenase independent mechanism (1995) Thromb. Haemost., 73, pp. 857-861
  • Cooray, P., Yuan, Y., Schoenwaelder, S.M., Salem, H.H., Jackson, S.P., Focal adhesion kinase (pp125FAK) cleavage and regulation by calpain (1996) Biochem. J., 318, pp. 41-47
  • Shattil, S.J., Cunningham, M., Wiedmer, T., Zhao, J., Sims, P.J., Brass, L.F., Regulation of GP lib Ilia receptor function studied with platelets permeabilized by the pore-forming complement proteins C5b-9 (1992) J. Biol. Chem., 267, pp. 18424-18431
  • Shattil, S., Haimovich, B., Cunningham, M., Lipfert, L., Parsons, J.T., Ginsberg, H., Brugge, J.S., Tyrosine phosphorilation of pp125FAK in platelets requires coordinated signaling through integrin and agonist receptors (1994) J. Biol. Chem., 265, pp. 14738-14745
  • Hui, K.Y., Jakubowski, J.A., Wyss, V.L., Angleton, E.L., Minimal sequence requirement of thrombin receptor agonist peptide (1992) Biochem. Biophys. Res. Commun., 184, pp. 790-796
  • Brass, L.F., Vassallo, R.R., Belmonte, E., Ahuja, M., Cichowski, K., Hoxie, J.A., Structure and function of the human platelet thrombin receptor: Studies using monoclonal antibodies against a defined etitope within the receptor N-terminus (1992) J. Biol. Chem., 267, pp. 13795-13798
  • Bahou, W.F., Coller, B.S., Potter, C.L., Norton, K.J., Kutok, J.L., Goligorsky, M.S., The thrombin receptor extracellular domain contains sites crucial for peptide ligand-induced activation (1993) J. Clin. Invest., 91, pp. 1405-1413
  • Natarajan, S., Riexinger, D., Gamberdella, M., Seiler, S., Tethered ligand penta peptide agonist of thrombin receptor: A study of side chain requirements for platelet activation (1995) Int. J. Pept. Protein. Res., 45, pp. 145-151
  • Nose, T., Shimohigashi, Y., Ohno, M., Costa, T., Shimizu, N., Ogino, Y., Enhancement of thrombin receptor activation by thrombin receptor derivated heptapeptide with para fluorophenylalanine in place of phenylalaine (1993) Biochem. Biophys. Res. Commun., 193, pp. 694-699
  • Shimohigashi, Y., Nose, T., Okazaki, M., Ohno, M., Costa, T., Shimizu, N., Ogino, Y., Differential roles of two consecutive phenylalanine residues in thrombin receptor-tethered ligand peptides (SFLLRNP) in thrombin receptor activation (1994) Biochem. Biophys. Res. Commun., 203, pp. 366-372
  • Rasmussen, U.B., Gachet, C., Schlesinger, Y., Hanau, D., Ohlmann, P., Van Obberghenschilling, E., Pouyssegur, J., Pavirani, A., A peptide ligand of the human alpha thrombin receptor antagonizes thrombin and partially activates platelets (1993) J. Biol. Chem., 268, pp. 14322-14328
  • Scarborough, R.M., Naughton, M.A., Teng, W., Jung, D.T., Rose, J.W., Vu, T.K., Wheaton, V.I., Coughlin, S.R., Tethered ligand agonist peptide. Structural requirements for thrombin receptor activation reveal mechanism of proteolytic unmasking of agonist function (1992) J. Biol. Chem., 267, pp. 13146-13149

Citas:

---------- APA ----------
Rossi, E.B. & Vizcargüenaga, M.I. (1999) . Activación plaquetaria por trombina. Acta Bioquimica Clinica Latinoamericana, 33(1), 35-47.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v33_n1_p35_Rossi [ ]
---------- CHICAGO ----------
Rossi, E.B., Vizcargüenaga, M.I. "Activación plaquetaria por trombina" . Acta Bioquimica Clinica Latinoamericana 33, no. 1 (1999) : 35-47.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v33_n1_p35_Rossi [ ]
---------- MLA ----------
Rossi, E.B., Vizcargüenaga, M.I. "Activación plaquetaria por trombina" . Acta Bioquimica Clinica Latinoamericana, vol. 33, no. 1, 1999, pp. 35-47.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v33_n1_p35_Rossi [ ]
---------- VANCOUVER ----------
Rossi, E.B., Vizcargüenaga, M.I. Activación plaquetaria por trombina. Acta Bioquim. Clin. Latinoam. 1999;33(1):35-47.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v33_n1_p35_Rossi [ ]