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Abstract:

Normospermic and pathological human seminal plasma (PSH) was analysed by affinity chromatography in Concanavalin-A Sepharose. Two main fractions were obtained, F1 and F2, in variable proportions and with different glycan content according with the type of pathology. Characterization of the oligosaccharide chains of F1 and F2 showed that galactose is always the main component together with fucose and variable amounts of mannose, N-acetylglucosamine and N-acetylgalactosamine. These fractions showed differences in the primary structure of glycoprotein glycans (N- and O-glycosidic-linked), according to the pathology. All the oligosaccharide chains in F1 correspond to mucin type glycoproteins (O-glycosidic type), whereas in F2 the molar ratio O-/N- is 5.3/1. F1 from azoospermic and oligozoospermic are nearly all of the O-glycosid type while in vasectomised the oligosaccharide chains differ from the other ones by a molar ratio O-/N- of 1.7/1. F2 present both O- and N- oligosaccharide chains in different amounts, with the only exception of vasectomised which belongs to O-glycosid type. The results obtained suggest that the composition of major PSH fractions from donors with different pathologies is characterized by marked structural differences on the glycan and the protein moiety.

Registro:

Documento: Artículo
Título:Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados
Autor:Tortorella, H.; Konrath, R.O.; Brandelli, A.; Furtado, J.L.F.; Mazzini, M.N.
Filiación:Lab. Fertilidade/Esterilidade Hum., Depatamento de Bioquimica, Univ. Federal do Rio Grande do Sul, 90050-170 Porto Alegre, Brazil
Depto. de Quim. Orgánica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Ciencias Químicas, Departamento de Bioquímica, Univ. Federal do Rio Grande do Sul, Brazil
Mestre en Química, Alumno Curso Doctorado Quim., Univ. do Estado de São Paulo, Brazil
Ciencias Químicas, Conselho Nac. Desenvolvimento C./T., Brazil
Licenciado en Química, Alumno Curso de Mestrado en Quim., Univ. Federal do Rio Grande do Sul, Brazil
Ciencias Químicas, Adjunta de Quim. Organ., Universidad de Buenos Aires
Departamento de Bioquimica, UFRGS, 90050-170 Porto Alegre - RS, Brazil
Palabras clave:Affinity chromatography; Carbohydrate; Glycoprotein; Lectin; Semen
Año:1996
Volumen:30
Número:1
Página de inicio:49
Página de fin:58
Título revista:Acta Bioquimica Clinica Latinoamericana
Título revista abreviado:Acta Bioquim. Clin. Latinoam.
ISSN:03252957
CODEN:ABCLD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella

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Citas:

---------- APA ----------
Tortorella, H., Konrath, R.O., Brandelli, A., Furtado, J.L.F. & Mazzini, M.N. (1996) . Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados. Acta Bioquimica Clinica Latinoamericana, 30(1), 49-58.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella [ ]
---------- CHICAGO ----------
Tortorella, H., Konrath, R.O., Brandelli, A., Furtado, J.L.F., Mazzini, M.N. "Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados" . Acta Bioquimica Clinica Latinoamericana 30, no. 1 (1996) : 49-58.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella [ ]
---------- MLA ----------
Tortorella, H., Konrath, R.O., Brandelli, A., Furtado, J.L.F., Mazzini, M.N. "Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados" . Acta Bioquimica Clinica Latinoamericana, vol. 30, no. 1, 1996, pp. 49-58.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella [ ]
---------- VANCOUVER ----------
Tortorella, H., Konrath, R.O., Brandelli, A., Furtado, J.L.F., Mazzini, M.N. Caracterización de fracciones mayoritarias del plasma seminal humano. Estudio comparative entre individuos normospérmicos, azoospérmicos, necrozoospérmicos, oligozoospérmicos y vasectomizados. Acta Bioquim. Clin. Latinoam. 1996;30(1):49-58.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252957_v30_n1_p49_Tortorella [ ]