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Pauza, N.L.; Sopenade Kracoff, Y.E.; Ferramola De Sancovich, A.M.; Sancovich, H.A. "In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis " (2004) Revista Argentina de Dermatologia. 85(3):154-163
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Abstract:

The chick embryo has been used as a screening system to study porphyrin metabolism, and the toxicological and teratogenic effects of many chemical substances. The aim of this study is to evaluate Cd 2+, Pb 2+, and Cu 2+ teratogenicity; and in vivo effects on chick embryo of d-aminolevulinic acid dehydratase (ALA-D) and porphobilinogen deaminase (PBG-D) activities. On day 12 of incubation, White Leghorn eggs were injected in the yolk with cadmium acetate, lead acetate, or copper sulfate in two different doses. The yolk sac membrane and liver were removed 12 and 60 hours later and ALA-D and PBG-D activities assayed. The in vivo Cd 2+ administration in chick embryo induced higher mortality rate and teratogenic effects than Pb 2+ and Cu 2+. The different effects observed on the enzyme activities can be better explained by both (a) differences in metals' capacity to cross the vitelline membrane, and (b) a secondary effect of alterations in general mineral metabolism, more than as a result of direct action on the enzymes themselves. Our results are the first to show that yolk sac membrane enzymes are more sensible than liver enzymes to the action of the metals used in our studies. These findings indicate that the yolk sac membrane is the preferential tissue to be used for metal biomarker toxicity assays.

Registro:

Documento: Artículo
Título:In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis
Autor:Pauza, N.L.; Sopenade Kracoff, Y.E.; Ferramola De Sancovich, A.M.; Sancovich, H.A.
Filiación:Catedra de Quimica Biologica, Argentina
Departamento de Quimica Biologica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:Chick embryo; D-aminolevulinic acid dehydratase; Haem; Metals; Porphobilinogen deaminase; biological marker; cadmium; cadmium acetate; copper; copper sulfate; heme; lead; lead acetate; liver enzyme; membrane enzyme; metal; porphobilinogen deaminase; porphobilinogen synthase; animal experiment; animal tissue; article; chick embryo; enzyme activity; enzyme assay; heme synthesis; liver; mineral metabolism; mortality; nonhuman; teratogenicity; vitelline membrane
Año:2004
Volumen:85
Número:3
Página de inicio:154
Página de fin:163
Título revista:Revista Argentina de Dermatologia
Título revista abreviado:Rev. Argent. Dermatol.
ISSN:03252787
CODEN:RADEB
CAS:cadmium, 22537-48-0, 7440-43-9; cadmium acetate, 543-90-8; copper, 15158-11-9, 7440-50-8; copper sulfate, 7758-98-7, 7758-99-8; heme, 14875-96-8; lead, 13966-28-4, 7439-92-1; lead acetate, 15347-57-6, 301-04-2; porphobilinogen deaminase, 9036-47-9, 9074-91-3; porphobilinogen synthase, 9036-37-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252787_v85_n3_p154_Pauza

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Citas:

---------- APA ----------
Pauza, N.L., Sopenade Kracoff, Y.E., Ferramola De Sancovich, A.M. & Sancovich, H.A. (2004) . In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis . Revista Argentina de Dermatologia, 85(3), 154-163.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252787_v85_n3_p154_Pauza [ ]
---------- CHICAGO ----------
Pauza, N.L., Sopenade Kracoff, Y.E., Ferramola De Sancovich, A.M., Sancovich, H.A. "In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis " . Revista Argentina de Dermatologia 85, no. 3 (2004) : 154-163.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252787_v85_n3_p154_Pauza [ ]
---------- MLA ----------
Pauza, N.L., Sopenade Kracoff, Y.E., Ferramola De Sancovich, A.M., Sancovich, H.A. "In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis " . Revista Argentina de Dermatologia, vol. 85, no. 3, 2004, pp. 154-163.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252787_v85_n3_p154_Pauza [ ]
---------- VANCOUVER ----------
Pauza, N.L., Sopenade Kracoff, Y.E., Ferramola De Sancovich, A.M., Sancovich, H.A. In vivo studies of Pb 2+, Cd 2+ and Cu 2+ toxicity and their effects on haem biosynthesis . Rev. Argent. Dermatol. 2004;85(3):154-163.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03252787_v85_n3_p154_Pauza [ ]