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Abstract:

Intracellular levels of cAMP and specific activities of adenylate cyclase and cAMP phosphodiesterase were measured during yeast-to-hyphae transition in the dimorphic fungus M. rouxii. Enzymatic activities were measured in permeabilized cells under conditions preventing protein dephosphorylation and proteolysis. A two-fold decrease in intracellular cAMP levels occurred shortly after exposure of the yeast to air quite before morphological changes became evident. Morphogenesis to hyphae after exposure to air was inhibited by the addition of 10 mM dibutyryl cAMP to the culture medium, and the yeast morphology was maintained for at least 24 hours. The decrease in cAMP levels that occurs shortly after exposure of yeast culture to air was mainly accounted for by variations in the state of activation of cAMP phosphodiesterase while the specific activity of adenylate cyclase did not vary significantly during yeast-to-hyphae transition. © 1983.

Registro:

Documento: Artículo
Título:cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii
Autor:Cantore, M.L.; Galvagno, M.A.; Passeron, S.
Filiación:Programa de Regulación Hormonal y Metabólica, Departamento de Química Biológica y Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Career Investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Palabras clave:3',5' cyclic nucleotide phosphodiesterase; adenylate cyclase; bucladesine; cyclic AMP; article; comparative study; drug effect; kinetics; metabolism; Mucor; physiology; species difference; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Adenylate Cyclase; Bucladesine; Comparative Study; Cyclic AMP; Kinetics; Mucor; Species Specificity; Support, Non-U.S. Gov't
Año:1983
Volumen:7
Número:11
Página de inicio:947
Página de fin:954
DOI: http://dx.doi.org/10.1016/0309-1651(83)90214-X
Título revista:Cell Biology International Reports
Título revista abreviado:Cell Biol. Int. Rep.
ISSN:03091651
CODEN:CBRPD
CAS:3',5' cyclic nucleotide phosphodiesterase, 9040-59-9; adenylate cyclase, 9012-42-4; bucladesine, 16980-89-5, 362-74-3; cyclic AMP, 60-92-4; 3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Adenylate Cyclase, EC 4.6.1.1; Bucladesine, 362-74-3; Cyclic AMP, 60-92-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v7_n11_p947_Cantore

Referencias:

  • Cantore, Galvagno, Passeron, Variations in the levels of cyclic adenosine 3′–5′ monophosphate and in the activities of adenylate cyclase and cyclic adenosine 3′–5′ monophosphate phosphodiesterase during aerobic morphogenesis of Mueor rouxii (1980) Archives of Biochemistry and Biophysics, 199, pp. 312-320
  • Cantore, Passeron, Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase (1982) Archives of Biochemistry and Biophysics, 219, pp. 1-11
  • Galvagno, Moreno, Cantore, Passeron, Cyclic adenosine 3′–5′ monophosphate phosphodiesterase from Mucor rouxii: regulation of enzyme activity by phosphorylation and dephosphorylation (1979) Biochemical and Biophysical Research Communications, 89, pp. 779-785
  • Larsen, Sypherd, Cyclic adenosine 3′,5′-monophosphate and morphogenesis in Mucor racemosus (1974) Journal of Bacteriology, 117, pp. 432-438
  • Lowry, Rosebrough, Farr, Randall, Protein measurement with Folin phenol reagent (1951) Journal of Biological Chemistry, 193, pp. 265-275
  • Maggese, Galvagno, Cantore, Passeron, In situ measurement of cAMP related enzymes in the dimorphic fungus Mucor rouxii (1982) Cell Biology International Reports, 6, pp. 1101-1108
  • Moreno, Galvagno, Passeron, Control of Mucor rouxii adenosine 3′–5′ monophosphate phosphodiesterase by phosphorylation-dephosphorylation and proteolysis (1982) Archives of Biochemistry and Biophysics, 214, pp. 573-580
  • Paveto, Epstein, Passeron, Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii (1975) Archives of Biochemistry and Biophysics, 169, pp. 449-456
  • Sypherd, Borgia, Paznokas, Biochemistry of dimorphism in the fungus Mucor (1978) Advances in Microbiological Physiology, 18, pp. 67-104
  • Tsang, Lehotay, Pearson Murphy, Competitive binding assay for adenosine 3′,5′-monophosphate employing a bovine adrenal protein: application to urine, plasma and tissues (1972) The Journal of Clinical Endocrinology and Metabolism, 35, pp. 809-817

Citas:

---------- APA ----------
Cantore, M.L., Galvagno, M.A. & Passeron, S. (1983) . cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii. Cell Biology International Reports, 7(11), 947-954.
http://dx.doi.org/10.1016/0309-1651(83)90214-X
---------- CHICAGO ----------
Cantore, M.L., Galvagno, M.A., Passeron, S. "cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii" . Cell Biology International Reports 7, no. 11 (1983) : 947-954.
http://dx.doi.org/10.1016/0309-1651(83)90214-X
---------- MLA ----------
Cantore, M.L., Galvagno, M.A., Passeron, S. "cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii" . Cell Biology International Reports, vol. 7, no. 11, 1983, pp. 947-954.
http://dx.doi.org/10.1016/0309-1651(83)90214-X
---------- VANCOUVER ----------
Cantore, M.L., Galvagno, M.A., Passeron, S. cAMP levels and in situ measurement of adenylate cyclase and cAMP phosphodiesterase activities during yeast-to-hyphae transition in the dimorphic fungus Mucor rouxii. Cell Biol. Int. Rep. 1983;7(11):947-954.
http://dx.doi.org/10.1016/0309-1651(83)90214-X