Abstract:
The role of protein kinase C (PKC) on muscarinic regulation of serotonin release in the pineal gland was investigated by measuring the pineal-PKC activity and serotonin secretion in response to muscarinic agents. Pineal slices, short-term incubated (0-15 min) without additions produced a low serotonin release and 20 to 24 percent PKC activity was found associated with membrane fractions. Prolonged exposure of pineal slices (30-180 min) produced further translocation of PKC activity to the membranes and a significant increase of serotonin release. Short-term treatment with pilocarpine and carbachol, stimulated PKC activity of both cytosolic and particulate fractions and the release of pineal serotonin. The pilocarpine effect was blocked by atropine indicating that it was mediated by muscarinic receptors. The present data support that PKC activation correlates with the increase of serotonin release by muscarinic agonist in pineal gland. © 1991.
Registro:
Documento: |
Artículo
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Título: | Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland |
Autor: | Finocchiaro, L.M.E.; Téllez-Iñón, M.T. |
Filiación: | Laboratorio de Sustancias Vasoactivas, Institute de Investigaciones Médicas, Universidad de Buenos Aires, Donate Alvarez 3150, 1427 Buenos Aires, Argentina Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Obligado 2490, 1428 Buenos Aires, Argentina
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Palabras clave: | muscarinic activation; pineal gland; protein kinase C; serotonin; angiotensin; atropine; carbachol; isoprenaline; muscarinic agent; muscarinic receptor; pilocarpine; protein kinase c; serotonin; staurosporine; animal experiment; animal tissue; article; cholinergic activity; controlled study; enzyme activity; long term exposure; male; metabolic regulation; nonhuman; pineal body; priority journal; rat; serotonin release; Angiotensin II; Animal; Atropine; Carbachol; Cell Membrane; Cytoplasm; Isoproterenol; Male; Parasympathomimetics; Pilocarpine; Pineal Gland; Protein Kinase C; Rats; Rats, Inbred Strains; Serotonin; Support, Non-U.S. Gov't |
Año: | 1991
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Volumen: | 15
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Número: | 10
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Página de inicio: | 943
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Página de fin: | 954
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DOI: |
http://dx.doi.org/10.1016/0309-1651(91)90144-8 |
Título revista: | Cell Biology International Reports
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Título revista abreviado: | Cell Biol. Int. Rep.
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ISSN: | 03091651
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CODEN: | CBRPD
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CAS: | Angiotensin II, 11128-99-7; Atropine, 51-55-8; Carbachol, 51-83-2; Isoproterenol, 7683-59-2; Parasympathomimetics; Pilocarpine, 92-13-7; Protein Kinase C, EC 2.7.1.37; Serotonin, 50-67-9
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v15_n10_p943_Finocchiaro |
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Citas:
---------- APA ----------
Finocchiaro, L.M.E. & Téllez-Iñón, M.T.
(1991)
. Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland. Cell Biology International Reports, 15(10), 943-954.
http://dx.doi.org/10.1016/0309-1651(91)90144-8---------- CHICAGO ----------
Finocchiaro, L.M.E., Téllez-Iñón, M.T.
"Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland"
. Cell Biology International Reports 15, no. 10
(1991) : 943-954.
http://dx.doi.org/10.1016/0309-1651(91)90144-8---------- MLA ----------
Finocchiaro, L.M.E., Téllez-Iñón, M.T.
"Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland"
. Cell Biology International Reports, vol. 15, no. 10, 1991, pp. 943-954.
http://dx.doi.org/10.1016/0309-1651(91)90144-8---------- VANCOUVER ----------
Finocchiaro, L.M.E., Téllez-Iñón, M.T. Effects of cholinergic muscarinic agents on protein kinase C activity in rat pineal gland. Cell Biol. Int. Rep. 1991;15(10):943-954.
http://dx.doi.org/10.1016/0309-1651(91)90144-8