Artículo
Grotewold, E.; Aisemberg, G.O.; Taccioli, G.E.; Judewicz, N.D. "Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa" (1990) Cell Biology International Reports. 14(1):69-78
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Abstract:
Wild-type Neurospora crassa grown in minimal medium was exposed to -difluormethyl ornithine (DFMO), a specific inhibitor of ornithine-decarboxylase (ODC-ase) activity. Protein-synthesis rates impaired by DFMO were restored by the addition of spermidine. The pattern on SDS-acrylamide gels displayed three newly synthesized polypeptides, p27, p31 and p99 after DFMO action in the absence of exogenous polyamine. The ODC-ase mutant (spe-1) grown in spermidine-supplemented medium did not show an induced polypeptide pattern. The lack of ODC-ase activity promotes the expression of p27- and p31-coding genes in both strains but transcription of p31 gene is shut-off after spermidine addition. Both transcripts are also accumulated after exposure to low cycloheximide doses or nutrient starvation. Another cycloheximide-inducible gene coding for p70 is also expressed under DFMO-treatment. © 1990.
Registro:
| Documento: | Artículo |
| Título: | Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa |
| Autor: | Grotewold, E.; Aisemberg, G.O.; Taccioli, G.E.; Judewicz, N.D. |
| Filiación: | Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | gene activation; Neurospora crassa; ODC-ase inhibition; polyamine deficiency; ornithine decarboxylase; polyamine; article; fungus; gene expression; neurospora crassa; nonhuman; priority journal; Eflornithine; Enzyme Inhibitors; Gene Expression Regulation, Fungal; Neurospora; Neurospora crassa; Ornithine Decarboxylase; Polyamines; Spermidine; Fungi; Neurospora crassa |
| Año: | 1990 |
| Volumen: | 14 |
| Número: | 1 |
| Página de inicio: | 69 |
| Página de fin: | 78 |
| DOI: | http://dx.doi.org/10.1016/0309-1651(90)90072-7 |
| Título revista: | Cell Biology International Reports |
| Título revista abreviado: | Cell Biol. Int. Rep. |
| ISSN: | 03091651 |
| CODEN: | CBRPD |
| CAS: | Eflornithine, 70052-12-9; Enzyme Inhibitors; Ornithine Decarboxylase, EC 4.1.1.17; Polyamines; Spermidine, 124-20-9 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p69_Grotewold |
Referencias:
- Aisemberg, Grotewold, Taccioli, Judewicz, A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide (1989) Exp. Mycol, 13, pp. 121-128
- Aisemberg, Taccioli, Grotewold, Judewicz, A differential gene expression promoted by cycloheximide in Neurospora crassa (1987) Exp. Mycol, 11, pp. 122-127
- Chamberlain, Fluorographic detection of radioactivity in polyacrylamide gels with water-soluble fluors: Sodium salicylate (1979) Anal. Biochem, 98, pp. 132-135
- Davis, Krasner, DiGangi, Ristow, Distinct roles of putrescine and spermidine in the regulation of ornithine decarboxylase in Neurospora crassa (1985) Proc. Natl. Acad. Sci. USA, 82, pp. 4105-4109
- Eversole, DiGangi, Menees, Davis, Structural gene for ornithine decarboxylase in Neurospora crassa (1985) Mol. Cell. Biol, 5, pp. 1301-1306
- Grotewold, Taccioli, Aisemberg, Judewicz, Early response and induced tolerance to cycloheximide in Neurospora crassa (1989) Current Genetics, 15, pp. 429-434
- Kahana, Nathans, Isolation of cloned cDNA encoding mammalian ornithine decarboxylase (1984) Proc. Natl. Acad. Sci. USA, 81, pp. 3645-3649
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- Paulus, Cramer, Davis, Compartmentation of spermidine in Neurospora crassa (1983) J. Biol. Chem, 258, pp. 8608-8612
- Paulus, Kiyono, Davis, Polyamine-deficient Neurospora crassa mutants and synthesis of cadaverine (1982) J. Bacteriol, 152, pp. 291-297
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Citas:
---------- APA ----------
Grotewold, E., Aisemberg, G.O., Taccioli, G.E. & Judewicz, N.D. (1990) . Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa. Cell Biology International Reports, 14(1), 69-78.http://dx.doi.org/10.1016/0309-1651(90)90072-7
---------- CHICAGO ----------
Grotewold, E., Aisemberg, G.O., Taccioli, G.E., Judewicz, N.D. "Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa" . Cell Biology International Reports 14, no. 1 (1990) : 69-78.http://dx.doi.org/10.1016/0309-1651(90)90072-7
---------- MLA ----------
Grotewold, E., Aisemberg, G.O., Taccioli, G.E., Judewicz, N.D. "Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa" . Cell Biology International Reports, vol. 14, no. 1, 1990, pp. 69-78.http://dx.doi.org/10.1016/0309-1651(90)90072-7
---------- VANCOUVER ----------
Grotewold, E., Aisemberg, G.O., Taccioli, G.E., Judewicz, N.D. Genes responsive to the alteration of polyamine biosynthesis in Neurospora crassa. Cell Biol. Int. Rep. 1990;14(1):69-78.http://dx.doi.org/10.1016/0309-1651(90)90072-7
