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Abstract:

Intracellular levels of cAMP and specific activities of adenylate cyclase, cAMP phosphodiesterase and cAMP-dependent protein kinase were measured during filamentation in the dimorphic fungus Candida albicans. Enzymatic assays were performed in permeabilized cells under conditions prevented endogenous proteolysis. The variations observed in cAMP levels were mainly accounted for by variations in the specific activities of adenylate cyclase and cAMP phosphodiesterase at different stages during germ tube formation. cAMP-dependent protein kinase, measured with kemptide as exogenous substrate, was developmental regulated. Some properties of the enzymatic activities from cell-free extracts are described. © 1990.

Registro:

Documento: Artículo
Título:cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans
Autor:Egidy, G.; Paveto, C.; Passeron, S.; Galvagno, M.A.
Filiación:Departamento de Química Biológica, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Pabellón II, 1428 Buenos Aires, Argentina
Palabras clave:adenylate cyclase; cyclic amp; cyclic amp dependent protein kinase; cyclic amp phosphodiesterase; article; candida albicans; differentiation; fungus; morphology; nonhuman; priority journal; 3',5'-Cyclic-Nucleotide Phosphodiesterase; Adenylate Cyclase; Candida albicans; Cyclic AMP; Candida albicans; Fungi
Año:1990
Volumen:14
Número:1
Página de inicio:59
Página de fin:68
DOI: http://dx.doi.org/10.1016/0309-1651(90)90071-6
Título revista:Cell Biology International Reports
Título revista abreviado:Cell Biol. Int. Rep.
ISSN:03091651
CODEN:CBRPD
CAS:3',5'-Cyclic-Nucleotide Phosphodiesterase, EC 3.1.4.17; Adenylate Cyclase, EC 4.6.1.1; Cyclic AMP, 60-92-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03091651_v14_n1_p59_Egidy

Referencias:

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  • Cantore, Passeron, Kinetic properties, solubilization, and molecular characterization of Mucor rouxii adenylate cyclase (1982) Archives of Biochemistry and Biophysics, 219, pp. 1-11
  • Chiew, Shepherd, Sullivan, Regulation of chitin synthesis during germ-tube formation in Candida albicans (1980) Archives of Microbiology, 125, pp. 97-104
  • Dabrowa, Howard, Proline uptake in Candida albicans (1981) Journal of General Microbiology, 127, pp. 391-397
  • Egidy, Paveto, Passeron, Galvagno, Relationship between cyclic adenosine 3′:5′-monophosphate and germination inCandida albicans (1989) Experimental Mycology, 13. , (in press)
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Citas:

---------- APA ----------
Egidy, G., Paveto, C., Passeron, S. & Galvagno, M.A. (1990) . cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans. Cell Biology International Reports, 14(1), 59-68.
http://dx.doi.org/10.1016/0309-1651(90)90071-6
---------- CHICAGO ----------
Egidy, G., Paveto, C., Passeron, S., Galvagno, M.A. "cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans" . Cell Biology International Reports 14, no. 1 (1990) : 59-68.
http://dx.doi.org/10.1016/0309-1651(90)90071-6
---------- MLA ----------
Egidy, G., Paveto, C., Passeron, S., Galvagno, M.A. "cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans" . Cell Biology International Reports, vol. 14, no. 1, 1990, pp. 59-68.
http://dx.doi.org/10.1016/0309-1651(90)90071-6
---------- VANCOUVER ----------
Egidy, G., Paveto, C., Passeron, S., Galvagno, M.A. cAMP levels and in situ measurement of cAMP related enzymes during yeast-to-hyphae transition in Candida albicans. Cell Biol. Int. Rep. 1990;14(1):59-68.
http://dx.doi.org/10.1016/0309-1651(90)90071-6