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Abstract:

The applicability of a simple and rapid spectrophotometric assay using o-phthaldialdehyde (OPA) to determine reactive lysine in dairy products was evaluated. For this purpose the values of reactive lysine of model systems and dairy products obtained by this procedure were compared with those obtained by the 1-fluoro-2,4-dinitrobenzene (FDNB) method in dialyzed and non-dialyzed samples. As a reference the latter method was applied to dialyzed samples to avoid interference of sugars. The results obtained showed that the OPA procedure is reliable for use even in the presence of high levels of sugars and can also be used to evaluate lysine damage after Maillard-type reactions. © 1992.

Registro:

Documento: Artículo
Título:Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products
Autor:Vigo, M.S.; Malec, L.S.; Gomez, R.G.; Llosa, R.A.
Filiación:Departamento de Quimica Orgánica (Area Bromatologia), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:1 fluoro 2,4 dinitrobenzene; lysine; phthalaldehyde; article; dairy product; intermethod comparison; spectrophotometry
Año:1992
Volumen:44
Número:5
Página de inicio:363
Página de fin:365
DOI: http://dx.doi.org/10.1016/0308-8146(92)90269-8
Título revista:Food Chemistry
Título revista abreviado:Food Chem.
ISSN:03088146
CODEN:FOCHD
CAS:1 fluoro 2,4 dinitrobenzene, 25376-51-6, 70-34-8; lysine, 56-87-1, 6899-06-5, 70-54-2; phthalaldehyde, 643-79-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03088146_v44_n5_p363_Vigo

Referencias:

  • AOAC, (1980) Official Methods of Analysis, , 13th edn, Association of Official Analytical Chemists, Washington, DC
  • Booth, Problems in the determination of FDNB-available lysine (1971) J. Sci. Food Agric., 22, pp. 658-666
  • Carpenter, The estimation of the available lysine in animal protein foods (1960) Biochem J, 77, pp. 604-610
  • Church, Swaisgood, Porter, Catignani, Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins (1983) Journal of Dairy Science, 66, pp. 1219-1227
  • Erbersdobler, Anderson, Determination of available lysine by various procedures in Maillard-type products (1983) ACS Symp. Ser. 215 (Maillard React. Foods Nutr.), pp. 419-427
  • Goodno, Swaisgood, Catignani, A fluorimetric assay for available lysine in proteins (1981) Anal. Biochem., 115, pp. 203-211
  • Hall, Henderson, An improvement in the determination of available lysine in carbohydrate-rich samples (1979) The Analyst, 104, pp. 1097-1100
  • Hall, Trinder, Givens, Observations on the use of 2,4,6-trinitrobenzene sulfonic acid for the determination of available lysine in animal protein concentrates (1973) Analyst, 98, pp. 673-686
  • Hurrell, Carpenter, Mechanisms of heat damage in proteins: 4. The reactive lysine content of heat damaged material as measured in different ways (1974) Br. J. Nutr., 32, pp. 589-604
  • Hurrell, Carpenter, The estimation of available lysine in foodstuffs after Maillard reactions (1981) Progr. Food Nutr. Sci., 5, pp. 159-176
  • Posati, Holsinger, De Vilbiss, Pallansch, Factors affecting the determination of available lysine in whey with 246-trinitrobenzene sulfonic acid (1972) Journal of Dairy Science, 55, pp. 1660-1665
  • Roth, Fluorescence reaction for amino acids (1971) Anal Chem, 43, pp. 880-882
  • Svedas, Galaev, Borisov, Berezin, The interaction of amino acids with o-phthaldialdehyde: a kinetic study and spectrophotometric assay of the reaction product (1980) Anal. Biochem., 101, pp. 188-195
  • Swaisgood, Catignani, Digestibility of modified milk proteins: nutritional implications (1985) J. Dairy Sci., 68, pp. 2782-2790
  • Tomarelli, Yuhas, Fisher, Weaber, An HPLC method for the determination of reactive (available) lysine in milk and infant formulas (1985) J. Agric. Food Chem., 33, pp. 316-318

Citas:

---------- APA ----------
Vigo, M.S., Malec, L.S., Gomez, R.G. & Llosa, R.A. (1992) . Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products. Food Chemistry, 44(5), 363-365.
http://dx.doi.org/10.1016/0308-8146(92)90269-8
---------- CHICAGO ----------
Vigo, M.S., Malec, L.S., Gomez, R.G., Llosa, R.A. "Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products" . Food Chemistry 44, no. 5 (1992) : 363-365.
http://dx.doi.org/10.1016/0308-8146(92)90269-8
---------- MLA ----------
Vigo, M.S., Malec, L.S., Gomez, R.G., Llosa, R.A. "Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products" . Food Chemistry, vol. 44, no. 5, 1992, pp. 363-365.
http://dx.doi.org/10.1016/0308-8146(92)90269-8
---------- VANCOUVER ----------
Vigo, M.S., Malec, L.S., Gomez, R.G., Llosa, R.A. Spectrophotometric assay using o-phthaldialdehyde for determination of reactive lysine in dairy products. Food Chem. 1992;44(5):363-365.
http://dx.doi.org/10.1016/0308-8146(92)90269-8