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Abstract:

Desiccation, resulting from extremely dry environmental conditions, is a serious obstacle to the survival of organisms. Water is vital for the maintenance of intracellular structure and prevents the irreversible formation of aggregates, an occurrence leading to loss of cellular function. To characterize genetic variation in desiccation stress resistance (DSR) in Drosophila melanogaster Meigen, an intercontinental set of recombinant inbred lines (RIL) is used. Flies are exposed to a low humidity environment (<10% relative humidity) at a constant temperature of 25°C. Desiccation stress resistance is higher in RIL derived from a backcross to the parental stock sensitive to heat stress (from Denmark) than in RIL derived from the reciprocal backcross to the heat-stress resistant stock (from Australia). Composite interval mapping reveals significant quantitative trail loci (QTL) for DSR in the set of RIL. Both major and minor effects QTL are detected, suggesting a complex genetic architecture. When compared with a previous investigation performed on the same set of RIL, the present study indicates that not all traits of resistance to environmental stressors are affected in the same direction by segregating co-localized QTL. © 2015 The Royal Entomological Society.

Registro:

Documento: Artículo
Título:Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster
Autor:Gomez, F.H.; Sambucetti, P.D.; Loeschcke, V.; Norry, F.M.
Filiación:Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires-IEGEBA (CONICET-UBA), Buenos Aires, Argentina
Department of Bioscience, Aarhus University, Aarhus C, Denmark
Palabras clave:Dehydration stress; Environmental stress; Small insects; Thermal stress; dehydration; desiccation; environmental conditions; environmental stress; fly; genetic structure; genetic variation; humidity; inbreeding; recombination; survival; temperature effect; Drosophila melanogaster; Hexapoda
Año:2015
Volumen:40
Número:3
Página de inicio:205
Página de fin:211
DOI: http://dx.doi.org/10.1111/phen.12103
Título revista:Physiological Entomology
Título revista abreviado:Physiol.Entomol.
ISSN:03076962
CODEN:PENTD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03076962_v40_n3_p205_Gomez

Referencias:

  • Arias, L.N., Sambucetti, P., Scannapieco, A.C., Survival to heat stress with and without heat-hardening in Drosophila melanogaster: interactions with larval density (2012) Journal of Experimental Biology, 13, pp. 2220-2225
  • Bradley, T.J., Williams, A.E., Rose, M.R., Physiological responses to selection for desiccation resistance in Drosophila melanogaster (1999) American Zoologist, 39, pp. 337-345
  • Coast, G.M., Orchard, I., Phillips, J.E., Schooley, D.A., Insect diuretic and antidiuretic hormones (2002) Advances in Insect Physiology, 29, pp. 279-409
  • Defays, R., Gómez, F.H., Sambucetti, P., Quantitative trait loci for longevity in heat-stressed Drosophila melanogaster (2011) Experimental Gerontology, 46, pp. 819-826
  • Dupuis, J., Siegmund, D., Statistical methods for mapping quantitative trait loci from a dense set of markers (1999) Genetics, 151, pp. 373-386
  • Geffen, E., Marlon, A.J., Gibbs, A.G., Selection for desiccation resistance in adult Drosophila melanogaster affects larval development and metabolite accumulation (2006) Journal of Experimental Biology, 209, pp. 3293-3300
  • Gibbs, A.G., Chippindale, A.K., Rose, M.R., Physiological mechanisms of evolved desiccation resistance in Drosophila melanogaster (1997) Journal of Experimental Biology, 200, pp. 1821-1832
  • Gibbs, A.G., Fukuzato, F., Matzkin, M., Evolution of water conservation mechanisms in Drosophila (2003) Journal of Experimental Biology, 206, pp. 1183-1192
  • Gomez, F.H., Defays, R., Sambucetti, P., Quantitative trait locus for starvation resistance in an intercontinental set of mapping populations of Drosophila melanogaster (2009) Fly, 3, pp. 246-252
  • Hoffmann, A.A., Harshman, L.G., Desiccation and starvation resistance in Drosophila: patterns of variation at the species, population and intrapopulation levels (1999) Heredity, 83, pp. 637-643
  • Hoffmann, A.A., Parsons, P.A., Selection for adult desiccation resistance in Drosophila melanogaster: fitness components, larval resistance and stress correlations (1993) Biological Journal of the Linnean Society of London, 48, pp. 43-54
  • Jordan, K.W., Morgan, T.J., Mackay, T.F.C., Quantitative trait loci for locomotor behavior in Drosophila melanogaster (2006) Genetics, 174, pp. 271-284
  • Kahsai, L., Kapan, N., Dircksen, H., Metabolic stress responses in Drosophila are modulated by brain neurosecretory cells that produce multiple neuropeptides (2010) PLoS One, 5, p. e11480
  • Kristensen, T.N., Barker, J.S.F., Pedersen, K.S., Loeschcke, V., Extreme temperatures increase the deleterious consequences of inbreeding under laboratory and semi-natural conditions (2008) Proceedings of the Royal Society of London Series B, Biological Sciences, 275, pp. 2055-2061
  • Lehmann, F.O., Schutzner, P., The respiratory basis of locomotion in Drosophila (2010) Journal of Insect Physiology, 56, pp. 543-550
  • Loeschcke, V., Kristensen, T.N., Norry, F.M., Consistent effects of a major QTL for thermal resistance in field-released Drosophila melanogaster (2011) Journal of Insect Physiology, 57, pp. 1227-1231
  • Mackay, T.F.C., Richards, S., Stone, E.A., The Drosophila melanogaster genetic reference panel (2012) Nature, 482, pp. 173-178
  • Nghiem, D., Gibbs, A.G., Rose, M.R., Bradley, T.J., Postponed aging and desiccation resistance in Drosophila melanogaster (2000) Experimental Gerontology, 35, pp. 957-969
  • Norry, F.M., Gomez, F.H., Loeschcke, V., Knockdown resistance to heat stress and slow recovery from chill coma are genetically associated in a quantitative trait locus region of chromosome 2 in Drosophila melanogaster (2007) Molecular Ecology, 16, pp. 3274-3284
  • Norry, F.M., Scannapieco, A.C., Sambucetti, P., QTL for the thermotolerance effect of heat hardening, knockdown resistance to heat and chill-coma recovery in an intercontinental set of recombinant inbred lines of Drosophila melanogaster (2008) Molecular Ecology, 17, pp. 4570-4581
  • Sambucetti, P., Scannapieco, A., Loeschcke, V., Norry, F.M., Heat stress survival in the pre-adult stage of the life cycle in an intercontinental set of recombinant inbred lines of Drosophila melanogaster (2013) Journal of Experimental Biology, 216, pp. 2953-2959
  • Sinclair, B.J., Gibbs, A.G., Roberts, S.P., Gene transcription during exposure to, and recovery from, cold and desiccation stress in Drosophila melanogaster (2007) Insect Molecular Biology, 16, pp. 435-443
  • Sinclair, B.J., Nelson, S., Nilson, T.L., The effect of selection for desiccation resistance on cold tolerance of Drosophila melanogaster (2007) Physiological Entomology, 32, pp. 322-327
  • Sørensen, J.G., Kristensen, T.N., Loeschcke, V., The evolutionary and ecological role of heat shock proteins (2003) Ecology Letters, 6, pp. 1025-1037
  • Sørensen, J.G., Nielsen, M.M., Loeschcke, V., Gene expression profile analysis of Drosophila melanogaster selected for resistance to environmental stressors (2007) Journal of Evolutionary Biology, 20, pp. 1624-1636
  • Telonis-Scott, M., Gane, M., DeGaris, S., High resolution mapping of candidate alleles for desiccation resistance in Drosophila melanogaster under selection (2012) Molecular Biology and Evolution, 29, pp. 1335-1351
  • Wang, S., Basten, C.J., Zeng, Z.B., (2010) Windows QTL Cartographer 2.5, , Department of Statistics, North Carolina State University, Raleigh, North Carolina
  • Zeng, Z.B., Precision mapping of quantitative trait loci (1994) Genetics, 136, pp. 1457-1468

Citas:

---------- APA ----------
Gomez, F.H., Sambucetti, P.D., Loeschcke, V. & Norry, F.M. (2015) . Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster. Physiological Entomology, 40(3), 205-211.
http://dx.doi.org/10.1111/phen.12103
---------- CHICAGO ----------
Gomez, F.H., Sambucetti, P.D., Loeschcke, V., Norry, F.M. "Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster" . Physiological Entomology 40, no. 3 (2015) : 205-211.
http://dx.doi.org/10.1111/phen.12103
---------- MLA ----------
Gomez, F.H., Sambucetti, P.D., Loeschcke, V., Norry, F.M. "Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster" . Physiological Entomology, vol. 40, no. 3, 2015, pp. 205-211.
http://dx.doi.org/10.1111/phen.12103
---------- VANCOUVER ----------
Gomez, F.H., Sambucetti, P.D., Loeschcke, V., Norry, F.M. Patterns of variation in desiccation resistance in a set of recombinant inbred lines in Drosophila melanogaster. Physiol.Entomol. 2015;40(3):205-211.
http://dx.doi.org/10.1111/phen.12103