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Abstract:

This article describes part of a graduate course in Biochemistry which involves the practical study of metabolic regulation in eukaryotic cells. This comes after three months of lectures on this topic and in the laboratory practical students partially purify and characterise transducin from bovine retina.

Registro:

Documento: Artículo
Título:Biochemical characterization of transducin, the G-protein of bovine retina
Autor:Muschietti, J.P.; Martinetto, H.E.; Flawi, M.M.
Filiación:Inst. de Invest. en Ing. Genet. y B., Buenos Aires, Argentina
Fac. de Cie. Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490 (1428), Buenos Aires, Argentina
Palabras clave:transducin; article; experiment; protein analysis; protein isolation; protein purification; protein structure; retina; student
Año:1998
Volumen:26
Número:1
Página de inicio:77
Página de fin:81
DOI: http://dx.doi.org/10.1016/S0307-4412(97)00162-3
Título revista:Biochemical Education
Título revista abreviado:Biochem. Educ.
ISSN:03074412
CODEN:BIEDD
CAS:transducin, 94699-82-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03074412_v26_n1_p77_Muschietti

Referencias:

  • Linder, M.E., Gilman, A.G., (1992) Scientific American, 267, pp. 36-43
  • Neer, E.J., (1995) Cell, 80, pp. 249-257
  • Ui, M., (1990) ADP-ribosylating Toxins and G Proteins: Insights in Signal Transduction, pp. 45-77. , J. Moss and M. Vaughan, Eds. American Society for Microbiology, Washington, DC
  • Stryer, L., (1987) Scientific American, 257, pp. 32-40
  • Muschietti, J.P., Bianchini, G.M., Martinetto, H.E., Carricarte, V.C., Giusto, N., Farber, D., Torres, H.N., Flawiá, M.M., (1989) European Journal of Biochemistry, 185, pp. 205-210
  • Harlow, E., Lane, D., (1989) Antibodies: A Laboratory Manual, , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

Citas:

---------- APA ----------
Muschietti, J.P., Martinetto, H.E. & Flawi, M.M. (1998) . Biochemical characterization of transducin, the G-protein of bovine retina. Biochemical Education, 26(1), 77-81.
http://dx.doi.org/10.1016/S0307-4412(97)00162-3
---------- CHICAGO ----------
Muschietti, J.P., Martinetto, H.E., Flawi, M.M. "Biochemical characterization of transducin, the G-protein of bovine retina" . Biochemical Education 26, no. 1 (1998) : 77-81.
http://dx.doi.org/10.1016/S0307-4412(97)00162-3
---------- MLA ----------
Muschietti, J.P., Martinetto, H.E., Flawi, M.M. "Biochemical characterization of transducin, the G-protein of bovine retina" . Biochemical Education, vol. 26, no. 1, 1998, pp. 77-81.
http://dx.doi.org/10.1016/S0307-4412(97)00162-3
---------- VANCOUVER ----------
Muschietti, J.P., Martinetto, H.E., Flawi, M.M. Biochemical characterization of transducin, the G-protein of bovine retina. Biochem. Educ. 1998;26(1):77-81.
http://dx.doi.org/10.1016/S0307-4412(97)00162-3