Abstract:
Since the discovery that long-term memory is dependent on protein synthesis, several transcription factors have been found to participate in the transcriptional activity needed for its consolidation. Among them, NF-kappa B is a constitutive transcription factor whose nuclear activity has proven to be necessary for the consolidation of inhibitory avoidance in mice. This transcription factor has a wide distribution in the nervous system, with a well-reported presence in dendrites and synaptic terminals. Here we report changes in synaptosomal NF-kappa B localization and activity, during long-term memory consolidation. Activity comparison of synaptosomal and nuclear NF-kappa B, indicates different dynamics for both localizations. In this study we identify two pools of synaptosomal NF-kappa B, one obtained with the synaptoplasm (free fraction) and the second bound to the synaptosomal membranes. During the early steps of consolidation the first pool is activated, as the membrane associated transcription factor fraction increases and concomitantly the free fraction decreases. These results suggest that the activation of synaptic NF-kappa B and its translocation to membranes are part of the consolidation of long-term memory in mice. © 2015 IBRO.
Registro:
Documento: |
Artículo
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Título: | Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice |
Autor: | Salles, A.; Boccia, M.; Blake, M.; Corbi, N.; Passananti, C.; Baratti, C.M.; Romano, A.; Freudenthal, R. |
Filiación: | Laboratorio de Neurobiología de la Memoria, FBMC, FCEyN, UBA - IFIBYNE, CONICET, Buenos Aires, Argentina Laboratorio de Neurofarmacología de procesos de memoria, FFyB, UBA, Buenos Aires, Argentina Consiglio Nazionale delle Ricerche, Institute of Molecular Biology and Pathology, Department of Molecular Medicine, Sapienza University, Rome, Italy
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Palabras clave: | I kappa B alpha; Memory consolidation; NF-kappa B; Synapse; immunoglobulin enhancer binding protein; immunoglobulin enhancer binding protein; Rela protein, mouse; transcription factor RelA; animal experiment; animal tissue; Article; avoidance behavior; controlled study; enzyme activity; hippocampus; long term memory; male; memory consolidation; mouse; nerve cell plasticity; nonhuman; priority journal; protein localization; synaptic membrane; synaptoplasm; animal; avoidance behavior; cell nucleus; dendrite; electric shock; fluorescent antibody technique; foot; hippocampus; memory consolidation; metabolism; outbred strain; physiology; synapse; synaptosome; Western blotting; Animals; Animals, Outbred Strains; Avoidance Learning; Blotting, Western; Cell Nucleus; Dendrites; Electroshock; Fluorescent Antibody Technique; Foot; Hippocampus; Male; Memory Consolidation; Mice; NF-kappa B; Synapses; Synaptosomes; Transcription Factor RelA |
Año: | 2015
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Volumen: | 291
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Página de inicio: | 70
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Página de fin: | 80
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DOI: |
http://dx.doi.org/10.1016/j.neuroscience.2015.01.063 |
Título revista: | Neuroscience
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Título revista abreviado: | Neuroscience
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ISSN: | 03064522
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CODEN: | NRSCD
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CAS: | NF-kappa B; Rela protein, mouse; Transcription Factor RelA
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v291_n_p70_Salles |
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Citas:
---------- APA ----------
Salles, A., Boccia, M., Blake, M., Corbi, N., Passananti, C., Baratti, C.M., Romano, A.,..., Freudenthal, R.
(2015)
. Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice. Neuroscience, 291, 70-80.
http://dx.doi.org/10.1016/j.neuroscience.2015.01.063---------- CHICAGO ----------
Salles, A., Boccia, M., Blake, M., Corbi, N., Passananti, C., Baratti, C.M., et al.
"Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice"
. Neuroscience 291
(2015) : 70-80.
http://dx.doi.org/10.1016/j.neuroscience.2015.01.063---------- MLA ----------
Salles, A., Boccia, M., Blake, M., Corbi, N., Passananti, C., Baratti, C.M., et al.
"Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice"
. Neuroscience, vol. 291, 2015, pp. 70-80.
http://dx.doi.org/10.1016/j.neuroscience.2015.01.063---------- VANCOUVER ----------
Salles, A., Boccia, M., Blake, M., Corbi, N., Passananti, C., Baratti, C.M., et al. Hippocampal dynamics of synaptic NF-kappa B during inhibitory avoidance long-term memory consolidation in mice. Neuroscience. 2015;291:70-80.
http://dx.doi.org/10.1016/j.neuroscience.2015.01.063