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Abstract:

Corticotropin-releasing hormone (CRH) coordinates neuroendocrine and behavioral adaptations to stress. Acute CRH administration in vivo activates extracellular signal-regulated kinase 1/2 (ERK1/2) in limbic brain areas, acting through the CRH receptor type 1 (CRH-R1). In the present study, we used CRH-COE-Cam mice that overexpress CRH in limbic-restricted areas, to analyze the effect of chronic CRH overexpression on ERK1/2 activation. By immunohistochemistry and confocal microscopy analysis we found that pERK1/2 levels in the basolateral amygdala (BLA) were similar in control and CRH overexpressing mice under basal conditions. Acute stress caused comparably increased levels of corticosterone in both control (CRH-COEcon-Cam) and CRH overexpressing (CRH-COEhom-Cam) animals. CRH-COEhom-Cam mice after stress showed reduced pERK1/2 immunoreactivity in the BLA compared to CRH-COEhom-Cam animals under basal conditions. Radioligand binding and in situ hybridization revealed higher density of CRH-R1 in the amygdala of CRH-COEhom mice under basal conditions compared to control littermates. A significant reduction of the receptor levels was observed in this area after acute stress, suggesting that stress may trigger CRH-R1 internalization/downregulation in these CRH overexpressing mice. Chronic CRH overexpression leads to reduced ERK1/2 activation in response to acute stress in the BLA. © 2009 IBRO.

Registro:

Documento: Artículo
Título:Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions
Autor:Silberstein, S.; Vogl, A.M.; Refojo, D.; Senin, S.A.; Wurst, W.; Holsboer, F.; Deussing, J.M.; Arzt, E.
Filiación:Laboratorio de Fisiología y Biología Molecular, Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Pab. II, 1428 Buenos Aires, Argentina
Max Planck Institute of Psychiatry, Kraepelinstraße 2-10, 80804 Munich, Germany
Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany
Palabras clave:CRF; CRH; CRH receptor; ERK; HPA axis; mitogen activated protein kinase 1; mitogen activated protein kinase 3; acute stress; amygdaloid nucleus; animal experiment; article; brain region; controlled study; immunohistochemistry; in situ hybridization; mouse; nonhuman; priority journal; protein expression; Amphibian Proteins; Amygdala; Animals; Autoradiography; Corticosterone; Corticotropin-Releasing Hormone; Disease Models, Animal; Gene Expression Regulation, Enzymologic; Iodine Isotopes; Male; Mice; Mice, Transgenic; Mitogen-Activated Protein Kinase 3; Peptide Hormones; Protein Binding; Radioimmunoassay; Receptors, Corticotropin-Releasing Hormone; Restraint, Physical; Stress, Psychological; Time Factors
Año:2009
Volumen:159
Número:2
Página de inicio:610
Página de fin:617
DOI: http://dx.doi.org/10.1016/j.neuroscience.2009.01.014
Título revista:Neuroscience
Título revista abreviado:Neuroscience
ISSN:03064522
CODEN:NRSCD
CAS:mitogen activated protein kinase 1, 137632-08-7; mitogen activated protein kinase 3, 137632-07-6; Amphibian Proteins; Corticosterone, 50-22-6; Corticotropin-Releasing Hormone, 9015-71-8; Iodine Isotopes; Mitogen-Activated Protein Kinase 3, 2.7.1.37; Peptide Hormones; Receptors, Corticotropin-Releasing Hormone; sauvagine, 74434-59-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v159_n2_p610_Silberstein

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Citas:

---------- APA ----------
Silberstein, S., Vogl, A.M., Refojo, D., Senin, S.A., Wurst, W., Holsboer, F., Deussing, J.M.,..., Arzt, E. (2009) . Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions. Neuroscience, 159(2), 610-617.
http://dx.doi.org/10.1016/j.neuroscience.2009.01.014
---------- CHICAGO ----------
Silberstein, S., Vogl, A.M., Refojo, D., Senin, S.A., Wurst, W., Holsboer, F., et al. "Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions" . Neuroscience 159, no. 2 (2009) : 610-617.
http://dx.doi.org/10.1016/j.neuroscience.2009.01.014
---------- MLA ----------
Silberstein, S., Vogl, A.M., Refojo, D., Senin, S.A., Wurst, W., Holsboer, F., et al. "Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions" . Neuroscience, vol. 159, no. 2, 2009, pp. 610-617.
http://dx.doi.org/10.1016/j.neuroscience.2009.01.014
---------- VANCOUVER ----------
Silberstein, S., Vogl, A.M., Refojo, D., Senin, S.A., Wurst, W., Holsboer, F., et al. Amygdaloid pERK1/2 in corticotropin-releasing hormone overexpressing mice under basal and acute stress conditions. Neuroscience. 2009;159(2):610-617.
http://dx.doi.org/10.1016/j.neuroscience.2009.01.014