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Abstract:

Long-term memory formation depends on protein and mRNA synthesis that subserves synaptic reorganization. The removal of pre-existing inhibitory proteins by the ubiquitin-proteasome system (UPS) is proposed as a crucial step to support these modifications. The activation of the constitutive transcription factor nuclear factor κB (NF-κB) depends on the degradation of the inhibitor of NF-κB (IκB) by the UPS. Here we study the effect of a UPS inhibitor, MG132, on long-term memory consolidation and NF-κB activation in the learning paradigm of the crab Chasmagnathus, a model in which this transcription factor plays a key role. Here we found that administration of MG132 interferes with long-term memory but not with short-term memory, and no facilitatory effects were found. Then we studied the effect of the UPS inhibitor on NF-κB pathway, finding that MG132 blocks the activation of NF-κB induced by training. These results suggest that the UPS is necessary for long-term memory consolidation, allowing for the activation of NF-κB as one of the target molecular pathways. © 2007 IBRO.

Registro:

Documento: Artículo
Título:Long-term memory consolidation depends on proteasome activity in the crab Chasmagnathus
Autor:Merlo, E.; Romano, A.
Filiación:Laboratorio de Neurobiología de la Memoria, Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, PAb. II, (1428EHA), Buenos Aires, Argentina
Palabras clave:consolidation; long-term memory; MG132; NF-κB; transcription factor; ubiquitin-proteasome system; benzyloxycarbonylleucylleucylleucinal; I kappa B; immunoglobulin enhancer binding protein; proteasome; ubiquitin; animal experiment; article; controlled study; crab; drug effect; drug inhibition; long term memory; male; nonhuman; priority journal; short term memory; Animals; Brachyura; Conditioning, Classical; Cysteine Proteinase Inhibitors; Dose-Response Relationship, Drug; Leupeptins; Male; Memory; Motor Activity; Neuronal Plasticity; NF-kappa B; Proteasome Endopeptidase Complex; Time Factors; Ubiquitin
Año:2007
Volumen:147
Número:1
Página de inicio:46
Página de fin:52
DOI: http://dx.doi.org/10.1016/j.neuroscience.2007.04.022
Título revista:Neuroscience
Título revista abreviado:Neuroscience
ISSN:03064522
CODEN:NRSCD
CAS:benzyloxycarbonylleucylleucylleucinal, 133407-82-6; proteasome, 140879-24-9; ubiquitin, 60267-61-0; benzyloxycarbonylleucyl-leucyl-leucine aldehyde, 133407-82-6; Cysteine Proteinase Inhibitors; Leupeptins; NF-kappa B; Proteasome Endopeptidase Complex, EC 3.4.25.1; Ubiquitin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v147_n1_p46_Merlo

Referencias:

  • Chain, D.G., Casadio, A., Schacher, S., Hegde, A.H., Valbrun, M., Yamamoto, N., Goldberg, A.L., Schwartz, J.H., Mechanisms for generating the autonomous cAMP-dependent protein kinase required for long-term facilitation in Aplysia (1999) Neuron, 22, pp. 147-156
  • Chain, D.G., Schwartz, J.H., Hegde, A.N., Ubiquitin-mediated proteolysis in learning and memory (2000) Mol Neurobiol, 20, pp. 125-142
  • Denny, J.B., Polan-Curtain, J., Ghuman, A., Wayner, M.J., Armstrong, D.L., Calpain inhibitors block long-term potentiation (1990) Brain Res, 534 (1-2), pp. 317-320
  • DiDonato, J.A., Hayakawa, M., Rothwarf, D.M., Zandi, E., Karin, M., A cytokine-responsive IκB kinase that activates the transcription factor NF-κB (1997) Nature, 388, pp. 548-554
  • DiDonato, J.A., Mercurio, F., Rosette, C., Wu-Li, J., Suyang, H., Ghosh, S., Karin, M., Mapping of the inducible IκB phosphorylation sites that signal its ubiquitination and degradation (1996) Mol Cell Biol, 16, pp. 1295-1304
  • Freudenthal, R., Boccia, M.M., Acosta, G.B., Blake, M.G., Merlo, E., Baratti, C.M., Romano, A., NF-kappaB transcription factor is required for inhibitory avoidance long-term memory in mice (2005) Eur J Neurosci, 21 (10), pp. 2845-2852
  • Freudenthal, R., Romano, A., Participation of Rel/NF-κB transcription factor in long-term memory in the crab Chasmagnathus (2000) Brain Res, 855, pp. 274-281
  • Freudenthal, R., Locatelli, F., Hermitte, G., Maldonado, H., Delorenzi, A., Romano, A., Kappa-B like DNA binding activity is enhanced after spaced training that induces long-term memory in the crab Chasmagnathus (1998) Neurosci Lett, 242, pp. 143-146
  • Gleeson, R.A., Zubkoff, P.L., The determination of hemolymph volume in the blue crab, Callinectes sapidus, utilizing 14C-thiocyanate (1977) Comp Biochem Physiol, 56 A, pp. 411-413
  • Hegde, A.N., Ubiquitin-proteasome-mediated local protein degradation and synaptic plasticity (2004) Prog Neurobiol, 73 (5), pp. 311-357
  • Hegde, A.N., Inokuchi, K., Pei, W., Casadio, A., Ghirardi, M., Chain, D.G., Martin, K.C., Schwartz, J.H., Ubiquitin C-terminal hydrolase is an immediate-early gene essential for long-term facilitation in Aplysia (1997) Cell, 89 (1), pp. 115-126
  • Hegde, A.N., Goldberg, A.L., Schwartz, J.H., Regulatory subunits of cAMP-dependent protein kinase are degraded after conjugation to ubiquitin: a molecular mechanism underlying long-term synaptic plasticity (1993) Proc Natl Acad Sci U S A, 90, pp. 7436-7440
  • Howell, D.C., (1987) Statistical methods of psychology. 2nd ed., , Duxbury, Boston
  • Lee, D.H., Goldberg, A.L., Proteasome inhibitors: valuable new tools for cell biologists (1998) Trends Cell Biol, 8 (10), pp. 397-403
  • Lopez-Salon, M., Alonso, M., Vianna, M.R.M., Viola, H., Mello e Souza, T., Izquierdo, I., Pasquini, J.M., Medina, J.H., The ubiquitin-proteasome cascade is required for mammalian long-term memory formation (2001) Eur J Neurosci, 14, pp. 1820-1826
  • Lozada, M., Romano, A., Maldonado, H., Long-term habituation to a danger stimulus in the crab Chasmagnathus (1990) Physiol Behav, 47, pp. 35-41
  • Meffert, M.K., Chang, J.M., Wiltgen, B.J., Fanselow, M.S., Baltimore, D., NF-kappa B functions in synaptic signaling and behavior (2003) Nat Neurosci, 6 (10), pp. 1072-1078
  • Mercurio, F., Zhu, H., Murray, B.W., Shevchenko, A., Bennet, B.L., Li, J., Young, D.B., Rao, A., IKK-1 and IKK-2 -cytokine-activated IκB kinases essential for NF-κB activation (1997) Science, 278, pp. 860-866
  • Merlo, E., Freudenthal, R., Romano, A., The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus (2002) Neuroscience, 112, pp. 161-172
  • Pedreira, M.E., Dimant, B., Tomsic, D., Quesada-Allue, L.A., Maldonado, H., Cycloheximide inhibits long-term habituation and context memory in the crab Chasmagnathus (1995) Pharmacol Biochem Behav, 52, pp. 385-395
  • Pedreira, M.E., Dimant, B., Maldonado, H., Inhibitors of protein and RNA synthesis block context memory and long-term habituation in the crab Chasmagnathus (1996) Pharmacol Biochem Behav, 54, pp. 611-617
  • Rock, K.L., Gramm, C., Rothstein, L., Clark, K., Stein, R., Dick, L., Hwang, D., Goldberg, A.L., Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules (1994) Cell, 78 (5), pp. 761-771
  • Roff, M., Thompson, J., Rodriguez, M.S., Jacque, J.M., Baleux, F., Arenzana-Seisdedos, F., Hay, R.T., Role of IκBα ubiquitination in signal-induced activation of NF-κB in vivo (1996) J Biol Chem, 271 (13), pp. 7844-7850
  • Romano, A., Lozada, M., Maldonado, H., Effects of naloxone pretreatment on habituation in the crab Chasmagnathus granulatus (1990) Behav Neural Biol, 53, pp. 113-122
  • Rosenthal, R., Rosnow, R.L., (1985) Contrast analysis focused comparisons in the analysis of variance, , Cambridge University Press, Cambridge, UK
  • Tanaka, K., Kawakami, T., Tateishi, K., Yashiroda, H., Chiba, T., Control of IκBα proteolysis by the ubiquitin-proteasome pathway (2001) Biochimie, 83, pp. 351-356
  • Upadhya, S.C., Ding, L., Smith, T.K., Hegde, A.N., Differential regulation of proteasome activity in the nucleus and the synaptic terminals (2006) Neurochem Int, 48, pp. 296-305
  • Upadhya, S.C., Smith, T.K., Hegde, A.N., Ubiquitin-proteasome-mediated CREB repressor degradation during induction of long-term facilitation (2004) J Neurochem, 91, pp. 210-219
  • Yeh, S.H., Lin, C.H., Lee, C.F., Gean, P.W., A requirement of nuclear factor-kappaB activation in fear-potentiated startle (2002) J Biol Chem, 277 (48), pp. 46720-46729
  • Yeh, S.H., Mao, S.C., Lin, H.C., Gean, P.W., Synaptic expression of glutamate receptor after encoding of fear memory in the rat amygdala (2006) Mol Pharmacol, 69 (1), pp. 299-308
  • Zhao, Y., Hegde, A.N., Martin, K.C., The ubiquitin proteasome system functions as an inhibitory constraint on synaptic strengthening (2003) Curr Biol, 13, pp. 887-898

Citas:

---------- APA ----------
Merlo, E. & Romano, A. (2007) . Long-term memory consolidation depends on proteasome activity in the crab Chasmagnathus. Neuroscience, 147(1), 46-52.
http://dx.doi.org/10.1016/j.neuroscience.2007.04.022
---------- CHICAGO ----------
Merlo, E., Romano, A. "Long-term memory consolidation depends on proteasome activity in the crab Chasmagnathus" . Neuroscience 147, no. 1 (2007) : 46-52.
http://dx.doi.org/10.1016/j.neuroscience.2007.04.022
---------- MLA ----------
Merlo, E., Romano, A. "Long-term memory consolidation depends on proteasome activity in the crab Chasmagnathus" . Neuroscience, vol. 147, no. 1, 2007, pp. 46-52.
http://dx.doi.org/10.1016/j.neuroscience.2007.04.022
---------- VANCOUVER ----------
Merlo, E., Romano, A. Long-term memory consolidation depends on proteasome activity in the crab Chasmagnathus. Neuroscience. 2007;147(1):46-52.
http://dx.doi.org/10.1016/j.neuroscience.2007.04.022