Artículo

González, S.L.; Labombarda, F.; González Deniselle, M.C.; Guennoun, R.; Schumacher, M.; De Nicola, A.F. "Progesterone up-regulates neuronal brain-derived neurotrophic factor expression in the injured spinal cord" (2004) Neuroscience. 125(3):605-614
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Abstract:

Progesterone (PROG) provides neuroprotection to the injured central and peripheral nervous system. These effects may be due to regulation of myelin synthesis in glial cells and also to direct actions on neuronal function. Recent studies point to neurotrophins as possible mediators of hormone action. Here, we show that the expression of brain-derived neurotrophic factor (BDNF) at both the mRNA and protein levels was increased by PROG treatment in ventral horn motoneurons from rats with spinal cord injury (SCI). Semiquantitative in situ hybridization revealed that SCI reduced BDNF mRNA levels by 50% in spinal motoneurons (control: 53.5±7.5 grains/mm2 vs. SCI: 27.5±1.2, P<0.05), while PROG administration to injured rats (4 mg/kg/day during 3 days, s.c.) elicited a three-fold increase in grain density (SCI+PROG: 77.8±8.3 grains/mm2, P<0.001 vs. SCI). In addition, PROG enhanced BDNF immunoreactivity in motoneurons of the lesioned spinal cord. Analysis of the frequency distribution of immunoreactive densities (χ2: 812.73, P<0.0001) showed that 70% of SCI+PROG motoneurons scored as dark stained whereas only 6% of neurons in the SCI group belonged to this density score category (P<0.001). PROG also prevented the lesion-induced chromatolytic degeneration of spinal cord motoneurons as determined by Nissl staining. In the normal intact spinal cord, PROG significantly increased BDNF inmunoreactivity in ventral horn neurons, without changes in mRNA levels. Our findings suggest that PROG enhancement of endogenous neuronal BDNF could provide a trophic environment within the lesioned spinal cord and might be part of the PROG activated-pathways to provide neuroprotection. © 2004 IBRO. Published by Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Progesterone up-regulates neuronal brain-derived neurotrophic factor expression in the injured spinal cord
Autor:González, S.L.; Labombarda, F.; González Deniselle, M.C.; Guennoun, R.; Schumacher, M.; De Nicola, A.F.
Filiación:Lab. of Neuroendocrine Biochemistry, Inst. de Biol. Y Med. Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Department of Biochemistry, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina
INSERM U488, Hôpital de Bicêtre, 80 R. du Gén. Leclerc, 94276 Kremlim-Bicêtre, Paris, France
Palabras clave:Analysis of variance; ANOVA; BDNF; Brain-derived neurotrophic factor; Control; CTL; ILIGV/area; Immunohistochemistry; In situ hybridization; Inverse logarithm of grain intensity per area; ISH; Neuroprotection; Neurotrophins; Steroid hormones; brain derived neurotrophic factor; messenger RNA; progesterone; animal cell; animal experiment; animal model; animal tissue; article; cell density; controlled study; density; gene expression; gene expression regulation; grain; immunoreactivity; in situ hybridization; male; motoneuron; neuroprotection; nonhuman; priority journal; protein blood level; rat; spinal cord atrophy; spinal cord injury; spinal cord lesion; spinal cord nerve cell; spinal cord ventral horn; upregulation; Animals; Brain-Derived Neurotrophic Factor; Disease Models, Animal; Immunohistochemistry; Male; Motor Neurons; Nerve Degeneration; Neuroprotective Agents; Progesterone; Rats; Rats, Sprague-Dawley; RNA, Messenger; Spinal Cord; Spinal Cord Injuries; Up-Regulation
Año:2004
Volumen:125
Número:3
Página de inicio:605
Página de fin:614
DOI: http://dx.doi.org/10.1016/j.neuroscience.2004.02.024
Título revista:Neuroscience
Título revista abreviado:Neuroscience
ISSN:03064522
CODEN:NRSCD
CAS:brain derived neurotrophic factor, 218441-99-7; progesterone, 57-83-0; Brain-Derived Neurotrophic Factor; Neuroprotective Agents; Progesterone, 57-83-0; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v125_n3_p605_Gonzalez

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Citas:

---------- APA ----------
González, S.L., Labombarda, F., González Deniselle, M.C., Guennoun, R., Schumacher, M. & De Nicola, A.F. (2004) . Progesterone up-regulates neuronal brain-derived neurotrophic factor expression in the injured spinal cord. Neuroscience, 125(3), 605-614.
http://dx.doi.org/10.1016/j.neuroscience.2004.02.024
---------- CHICAGO ----------
González, S.L., Labombarda, F., González Deniselle, M.C., Guennoun, R., Schumacher, M., De Nicola, A.F. "Progesterone up-regulates neuronal brain-derived neurotrophic factor expression in the injured spinal cord" . Neuroscience 125, no. 3 (2004) : 605-614.
http://dx.doi.org/10.1016/j.neuroscience.2004.02.024
---------- MLA ----------
González, S.L., Labombarda, F., González Deniselle, M.C., Guennoun, R., Schumacher, M., De Nicola, A.F. "Progesterone up-regulates neuronal brain-derived neurotrophic factor expression in the injured spinal cord" . Neuroscience, vol. 125, no. 3, 2004, pp. 605-614.
http://dx.doi.org/10.1016/j.neuroscience.2004.02.024
---------- VANCOUVER ----------
González, S.L., Labombarda, F., González Deniselle, M.C., Guennoun, R., Schumacher, M., De Nicola, A.F. Progesterone up-regulates neuronal brain-derived neurotrophic factor expression in the injured spinal cord. Neuroscience. 2004;125(3):605-614.
http://dx.doi.org/10.1016/j.neuroscience.2004.02.024