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Abstract:

Using intracellular recording, we studied how several muscarinic antagonists affected the evoked endplate potentials in singly and dually innervated endplates of the levator auris longus muscle from 3 to 6-day-old rats. In dually innervated fibers, a second endplate potential (EPP) may appear after the first one when we increase the stimulation intensity. The lowest and highest EPP amplitudes are designated "small-EPP" and "large-EPP," respectively. In singly innervated endplates and large-EPP, we found an inhibition of acetylcholine release by M1-receptor antagonists pirenzepine and MT-7 (more than 30%) and M2-receptor antagonists methoctramine and AF-DX 116 (more than 40%). The small-EPP was also inhibited by both M2-receptor antagonists methoctramine (approximately 70%) and AF-DX 116 (approximately 40%). However, the small-EPP was enhanced by M1-receptor antagonists pirenzepine (approximately 90%) and MT-7 (approximately 50%). The M4-receptor selective antagonists tropicamide and MT-3 can also increase the small-EPP amplitude (75% and 120%, respectively). We observed a graded change from a multichannel involvement (P/Q- N- and L-type voltage-dependent calcium channels) of all muscarinic responses (M1-, M2- and M4-mediated) in the small-EPP to the single channel (P/Q-type) involvement of the M1 and M2 responses in the singly innervated endplates. This indicates the existence of a progressive calcium channels shutoff in parallel with the specialization of the adult type P/Q channel. In conclusion, muscarinic autoreceptors can directly modulate large-EPP generating ending potentiation, and small-EPP generating ending depression through their association with the calcium channels during development. © 2003 IBRO. Published by Elsevier Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:Muscarinic autoreceptors related with calcium channels in the strong and weak inputs at polyinnervated developing rat neuromuscular junctions
Autor:Santafé, M.M.; Salon, I.; Garcia, N.; Lanuza, M.A.; Uchitel, O.D.; Tomàs, J.
Filiación:Unitat d'Histologia i Neurobiologia, Facultat Med. i Ciencies de la Salut, Universitat Rovira i Virgili, carrer St. Llorenç num 21, 43201 Reus, Spain
Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exactas y Naturales, Pabellón II, 1428 Buenos Aires, Argentina
Palabras clave:Cholinergic synapse; Motor endplate; Motor nerve terminal; Polyneuronal innervation; Voltage-dependent calcium channels; acetylcholine; calcium; calcium channel; calcium channel L type; calcium channel N type; calcium channel P type; calcium channel Q type; methoctramine; muscarinic M1 receptor antagonist; muscarinic M2 receptor antagonist; muscarinic M4 receptor; muscarinic receptor; muscarinic receptor blocking agent; otenzepad; pirenzepine; tropicamide; acetylcholine release; animal experiment; animal tissue; article; controlled study; drug effect; endplate potential; innervation; intracellular recording; neuromuscular synapse; neurotransmitter release; nonhuman; priority journal; rat
Año:2004
Volumen:123
Número:1
Página de inicio:61
Página de fin:73
DOI: http://dx.doi.org/10.1016/j.neuroscience.2003.09.012
Título revista:Neuroscience
Título revista abreviado:Neuroscience
ISSN:03064522
CODEN:NRSCD
CAS:acetylcholine, 51-84-3, 60-31-1, 66-23-9; calcium, 7440-70-2, 14092-94-5; methoctramine, 104807-46-7; otenzepad, 100158-38-1, 102394-31-0; pirenzepine, 28797-61-7, 29868-97-1; tropicamide, 1508-75-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v123_n1_p61_Santafe

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Citas:

---------- APA ----------
Santafé, M.M., Salon, I., Garcia, N., Lanuza, M.A., Uchitel, O.D. & Tomàs, J. (2004) . Muscarinic autoreceptors related with calcium channels in the strong and weak inputs at polyinnervated developing rat neuromuscular junctions. Neuroscience, 123(1), 61-73.
http://dx.doi.org/10.1016/j.neuroscience.2003.09.012
---------- CHICAGO ----------
Santafé, M.M., Salon, I., Garcia, N., Lanuza, M.A., Uchitel, O.D., Tomàs, J. "Muscarinic autoreceptors related with calcium channels in the strong and weak inputs at polyinnervated developing rat neuromuscular junctions" . Neuroscience 123, no. 1 (2004) : 61-73.
http://dx.doi.org/10.1016/j.neuroscience.2003.09.012
---------- MLA ----------
Santafé, M.M., Salon, I., Garcia, N., Lanuza, M.A., Uchitel, O.D., Tomàs, J. "Muscarinic autoreceptors related with calcium channels in the strong and weak inputs at polyinnervated developing rat neuromuscular junctions" . Neuroscience, vol. 123, no. 1, 2004, pp. 61-73.
http://dx.doi.org/10.1016/j.neuroscience.2003.09.012
---------- VANCOUVER ----------
Santafé, M.M., Salon, I., Garcia, N., Lanuza, M.A., Uchitel, O.D., Tomàs, J. Muscarinic autoreceptors related with calcium channels in the strong and weak inputs at polyinnervated developing rat neuromuscular junctions. Neuroscience. 2004;123(1):61-73.
http://dx.doi.org/10.1016/j.neuroscience.2003.09.012