Navegar

Colección

Datos Estadísticas

Artículo

El editor solo permite decargar el artículo en su versión post-print desde el repositorio. Por favor, si usted posee dicha versión, enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Evidence for the participation of Rel/NF-κB transcription factors in long-term memory has recently been reported in the context-signal learning paradigm of the crab Chasmagnathus, in which a high correlation between long-term memory formation and NF-κB activation was observed. Two components of the NF-κB pathway in the crab brain have now been identified by cross-immunoreactivity using mammalian antibodies for IκB-α and IκB kinase α. Furthermore, IκB kinase-like phosphotransferase activity, which was inhibited by the IκB kinase inhibitor sulfasalazine, was detected in brain extracts. We have evaluated the effect of sulfasalazine administration on long-term memory tested at 48 h. Amnesia was found when sulfasalazine was administered pre-training and 5 h after training but not at 0 or 24 h after training. Thus, two periods for sulfasalazine-induced amnesia were found in coincidence with the two phases of NF-κB activation previously described (immediately and 6 h after training). The cyclooxygenase inhibitor indomethacin did not induce amnesia when administered pre-training. Thus, the possibility that sulfasalazine induces amnesia by means of cyclooxygenase inhibition is unlikely to be tenable. In vivo sulfasalazine inhibition of basal NF-κB activity was found between 30 and 45 min after injection, as assessed by electrophoretic mobility shift assay. On the other hand, in vivo sulfasalazine administration 6 h after training inhibited the second phase of training-induced NF-κB activation, providing evidence that the sulfasalazine effect on memory is due to a direct effect of the drug on the NF-κB pathway. These results provide the first evidence that IκB kinase and NF-κB activation are necessary for memory formation. © 2002 IBRO. Published by Elsevier Science Ltd. All rights reserved.

Registro:

Documento: Artículo
Título:The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus
Autor:Merlo, E.; Freudenthal, R.; Romano, A.
Filiación:Facultad de Ciencias Exactas y Nat., Departamento de Ciencias Biologicas, Universidad de Buenos Aires, (1428) Buenos Aires, Argentina
Palabras clave:Crab; IκB kinase; long-term memory; Neural plasticity; Rel/NF-κB; I kappa B kinase; immunoglobulin enhancer binding protein; indometacin; phosphotransferase inhibitor; prostaglandin synthase; sulfalazine; enzyme inhibitor; I kappa B; I kappa B kinase; immunoglobulin enhancer binding protein; indometacin; prostaglandin synthase inhibitor; protein serine threonine kinase; salazosulfapyridine; amnesia; animal experiment; animal tissue; article; assay; brain; controlled study; crab; enzyme activation; enzyme inhibition; long term memory; male; nonhuman; priority journal; training; amnesia; animal; Brachyura; chemically induced disorder; drug antagonism; drug effect; escape behavior; habituation; memory; metabolism; physiology; time; Amnesia; Animals; Brachyura; Brain; Cyclooxygenase Inhibitors; Enzyme Inhibitors; Escape Reaction; Habituation, Psychophysiologic; I-kappa B Kinase; I-kappa B Proteins; Indomethacin; Male; Memory; NF-kappa B; Protein-Serine-Threonine Kinases; Sulfasalazine; Time Factors
Año:2002
Volumen:112
Número:1
Página de inicio:161
Página de fin:172
DOI: http://dx.doi.org/10.1016/S0306-4522(02)00049-0
Título revista:Neuroscience
Título revista abreviado:Neuroscience
ISSN:03064522
CODEN:NRSCD
CAS:Cyclooxygenase Inhibitors; Enzyme Inhibitors; I-kappa B Kinase, EC 2.7.1.37; I-kappa B Proteins; Indomethacin, 53-86-1; NF-kappa B; Protein-Serine-Threonine Kinases, EC 2.7.1.37; Sulfasalazine, 599-79-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03064522_v112_n1_p161_Merlo

Referencias:

  • Agranoff, B.W., (1972) The Chemistry of Mood Motivation and Memory, , Plenum, New York
  • Albensi, B.C., Mattson, M.P., Evidence for the involvement of TNF and NF-kappaB in hippocampal synaptic plasticity (2000) Synapse, 35, pp. 151-159
  • Alberini, C.M., Ghirardi, M., Metz, R., Kandel, E.R., C/EBP is an immediate-early gene required for the consolidation of long-term facilitation in Aplysia (1994) Cell, 76, pp. 1099-1114
  • Barondes, S.H., Protein synthesis-dependent and protein synthesis-independent memory storage processes (1975) Short-Term Memory, , Deutsch, D., Deutsch, J.A. (Eds.). Academic, New York
  • Bernabeu, R., Bevilaqua, L., Ardenghi, P., Bromberg, E., Schmitz, P., Bianchin, M., Izquierdo, I., Medina, J., Involvement of hippocampal cAMP/cAMP-dependent protein kinase signaling pathways in a late memory consolidation phase of aversively motivated learning in rats (1997) Proc. Natl. Acad. Sci. USA, 94, pp. 7041-7046
  • Bourtchuladze, R., Abel, T., Berman, N., Gordon, R., Lapidus, K., Kandel, E.R., Different training procedures recruit either one or two critical periods for contextual memory consolidation, each of which requires protein synthesis and PKA (1998) Learn. Mem., 5, pp. 365-374
  • Choi, J.-S., Kim, J., Kim, D.-H., Chun, M.-H., Gwag, B.-J., Yoon, S.K., Joo, C.-K., Failure to activate NF-kappaB promotes apoptosis of retinal ganglion cells following optic nerve transection (2000) Brain Res., 883, pp. 60-68
  • Crow, T., Forrester, J., Inhibition of protein synthesis blocks long-term enhancement of generator potentials produced by one-trial in vivo conditioning in Hermissenda (1990) Proc. Natl. Acad. Sci. USA, 87, pp. 4490-4494
  • Davis, H.P., Squire, L.R., Protein synthesis and memory: A review (1984) Psychol. Bull., 96, pp. 518-559
  • Deisseroth, K., Bito, H., Tsien, R.W., Signaling from synapse to nucleus: Postsynaptic CREB phosphorylation during multiple forms of hippocampal synaptic plasticity (1996) Neuron, 16, pp. 89-101
  • Delorenzi, A., Maldonado, H., Memory enhancement by the angiotensinergic system in the crab Chasmagnathus is mediated by endogenous angiotensin II (1999) Neurosci. Lett., 266, pp. 1-4
  • DiDonato, J.A., Hayakawa, M., Rothwarf, D.M., Zandi, E., Karin, M., A cytokine-responsive IκB kinase that activates the transcription factor NF-κB (1997) Nature, 388, pp. 548-554
  • DiDonato, J.A., Mercurio, F., Rosette, C., Wu-li, J., Suyang, H., Ghosh, S., Karin, M., Mapping of the inducible IκB phosphorylation sites that signal its ubiquitination and degradation (1996) Mol. Cell. Biol., 16, pp. 1295-1304
  • Escoubas, J.M., Briant, L., Montagnani, C., Hez, S., Devaux, C., Roch, P., Oyster IKK-like protein shares structural and functional properties with its mammalian homologues (1999) FEBS Lett., 453, pp. 293-298
  • Freeman, F.M., Rose, S.P.R., Scholey, A.B., Two time windows of anisomycin-induced amnesia for passive avoidance training in the day-old chick (1995) Neurobiol. Learn. Mem., 63, pp. 291-295
  • Freudenthal, R., Locatelli, F., Hermitte, G., Maldonado, H., Delorenzi, A., Romano, A., Kappa-B like DNA binding activity is enhanced after spaced training that induces long-term memory in the crab Chasmagnathus (1998) Neurosci. Lett., 242, pp. 143-146
  • Freudenthal, R., Romano, A., Participation of Rel/NF-κB transcription factor in long-term memory in the crab Chasmagnathus (2000) Brain Res., 855, pp. 274-281
  • Gleeson, R.A., Zubkoff, P.L., The determination of hemolymph volume in the blue crab, Callinectes sapidus, utilizing 14C-thiocyanate (1977) Comp. Biochem. Physiol., 56 A, pp. 411-413
  • Grilli, M., Pizzi, M., Memo, M., Spano, P., Neuroprotection by aspirin and sodium salicylate through blockade of NF-kappaB activation (1996) Science, 274, pp. 1383-1385
  • Guerrini, L., Blasi, F., Denis-Donini, S., Synaptic activation of NF-kappa B by glutamate in cerebellar granule neurons in vitro (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 9077-9081
  • Hermitte, G., Pedreira, M.E., Tomsic, D., Maldonado, H., Context shift and protein synthesis inhibition disrupt long-term habituation after spaced, but not massed, training in the crab Chasmagnathus (1999) Neurobiol. Learn. Mem., 71, pp. 34-49
  • Hoeger, U., Florey, E., Cathecholamine degradation in the hemolimph of the Chinese crab Eriocher sinensis (1989) Comp. Biochem. Physiol., 92, pp. 323-327
  • Holsher, C., Inhibitors of cyclooxigenases produce amnesia for a passive avoidance task in the chick (1995) Eur. J. Neurosci., 7, pp. 1360-1365
  • Holsher, C., Prostaglandins play a role in memory consolidation in the chick (1995) Eur. J. Pharmacol., 294, pp. 253-259
  • Hoult, J.R., Pharmacological and biochemical actions of sulphasalazine (1986) Drugs, 32, pp. 18-26
  • Howell, D.C., (1987) Statisticals Methods of Psychology, 2nd edn., , Duxbury, Boston, MA
  • Israel, A., The IKK complex: An integrator of all signals that activate NF-kappaB? (2000) Trends Cell Biol., 10, pp. 129-133
  • Kaang, B.K., Kandel, E., Grant, S.G.N., Activation of cAMP-responsive genes by stimuli that produce long-term facilitation in Aplysia sensory neurons (1993) Neuron, 10, pp. 427-435
  • Kaltschmidt, C., Kaltschmidt, B., Bauerle, P.A., Brain synapses contain inducible forms of the transcription factor NF-kappa B (1993) Mech. Dev., 43, pp. 135-147
  • Koop, E.B., Ghosh, S., Inhibition of NF-kappa B by sodium salicylate and aspirin (1994) Science, 265, pp. 956-959
  • Locatelli, F., Maldonado, H., Romano, A., Two critical periods for cAMP-dependent protein kinase activity during long-term memory consolidation in the crab Chasmagnathus (2001) Neurobiol. Learn. Mem., 77, pp. 234-249
  • Lozada, M., Romano, A., Maldonado, H., Long-term habituation to a danger stimulus in the crab Chasmagnathus (1990) Physiol. Behav., 47, pp. 35-41
  • Meberg, P.J., Kinney, W.R., Valcourt, E.G., Routtenberg, A., Gene expression of the transcription factor NF-B in hippocampus: Regulation by synaptic activity (1996) Mol. Brain Res., 38, pp. 179-190
  • Mercurio, F., Zhu, H., Murray, B.W., Shevchenko, A., Bennet, B.L., Li, J., Young, D.B., Rao, A., IKK-1 and IKK-2-cytokine-activated IκB kinases essential for NF-κB activation (1997) Science, 278, pp. 860-866
  • Newton, R., Kuitert, L.M.E., Bergman, M., Adcock, I.M., Barnes, P., Evidence for the involvement of NF-kappaB in the transcriptional control of COX-2 gene expression by IL-1beta (1997) Biochem. Biophys. Res. Commun., 237, pp. 28-32
  • Pedreira, M.E., Dimant, B., Tomsic, D., Quesada-Allue, L.A., Maldonado, H., Cycloheximide inhibits long-term habituation and context memory in the crab Chasmagnathus (1995) Pharmacol. Biochem. Behav., 52, pp. 385-395
  • Pedreira, M.E., Romano, A., Tomsic, D., Lozada, M., Maldonado, H., Massed and spaced training build up different components of long-term habituation in the crab Chasmagnathus (1998) Animal Learn. Behav., 26, pp. 34-45
  • Romano, A., Lozada, M., Maldonado, H., Effects of naloxone pretreatment on habituation in the crab Chasmagnathus granulatus (1990) Behav. Neur. Biol., 53, pp. 113-122
  • Rosenthal, R., Rosnow, R.L., (1985) Contrast Analysis Focused Comparisons in the Analysis of Variance, , Cambridge University Press, Cambridge
  • Rothwarf, D.M., Zandi, E., Natoli, G., Karin, M., IKK-γ is an essential regulatory subunit of the IκB kinase complex (1998) Nature, 395, pp. 297-300
  • Schneider, H., Pitossi, F., Balshun, D., Wagner, A., Del Rey, A., Besedovsky, H.O., A neuromodulatory role of interleukin-1beta in the hippocampus (1998) Proc. Natl. Acad. Sci. USA, 95, pp. 7778-7783
  • Tomsic, D., Pedreira, E.M., Romano, A., Hermitte, G., Maldonado, H., Association to context as a determinant of long-term habituation in the crab Chasmagnathus (1998) Animal Learn. Behav., 26, pp. 196-209
  • Wahl, C., Liptay, S., Adler, G., Schmid, R., Sulfasalazine: A potent and specific inhibitor of nuclear factor kappa B (1998) J. Clin. Invest., 101, pp. 1163-1174
  • Weber, C.K., Liptay, S., Wirth, T., Adler, G., Schmid, R.M., Suppression of NF-kappaB activity by sulfasalazine is mediated by direct inhibition of IkappaB kinases alpha and beta (2000) Gastroenterology, 119, pp. 1209-1218
  • Wellmann, H., Kaltschmidt, B., Kaltschmidt, C., Retrograde transport of transcription factor NF-kappaB in living neurons (2000) J. Biol. Chem., 276, pp. 11821-11829
  • Yamoaka, S., Courtois, G., Bessia, C., Whiteside, S.T., Weil, R., Agou, F., Kirk, H.E., Israel, A., Complementation cloning of NEMO, a component of the IκB kinase complex essential for NF-κB activation (1998) Cell, 93, pp. 1231-1240
  • Yin, J.C.P., Del Vecchio, M., Zhou, H., Tully, T., CREB as a memory modulator: Induced expression of a dCREB2 activator isoform enhances long-term memory in Drosophila (1995) Cell, 81, pp. 107-115
  • Yin, M.-J., Yamamoto, Y., Gaynor, R.B., The anti-inflammatory agents aspirin and salicylate inhibit the activity of IkappaB kinase-beta (1998) Nature, 396, pp. 77-80
  • Zandi, E., Rothwarf, D.M., Delhase, M., Hayakawa, M., Karin, M., The IκB kinase complex (IKK) contains two kinase subunits, IKK-α and IKK-β, necessary for IκB phosphorylation and NF-κB activation (1997) Cell, 91, pp. 243-252

Citas:

---------- APA ----------
Merlo, E., Freudenthal, R. & Romano, A. (2002) . The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus. Neuroscience, 112(1), 161-172.
http://dx.doi.org/10.1016/S0306-4522(02)00049-0
---------- CHICAGO ----------
Merlo, E., Freudenthal, R., Romano, A. "The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus" . Neuroscience 112, no. 1 (2002) : 161-172.
http://dx.doi.org/10.1016/S0306-4522(02)00049-0
---------- MLA ----------
Merlo, E., Freudenthal, R., Romano, A. "The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus" . Neuroscience, vol. 112, no. 1, 2002, pp. 161-172.
http://dx.doi.org/10.1016/S0306-4522(02)00049-0
---------- VANCOUVER ----------
Merlo, E., Freudenthal, R., Romano, A. The IκB kinase inhibitor sulfasalazine impairs long-term memory in the crab Chasmagnathus. Neuroscience. 2002;112(1):161-172.
http://dx.doi.org/10.1016/S0306-4522(02)00049-0