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Abstract:

The effect of in vitro glycation on δ-aminolevulinic dehydratase (ALA-D) under several experimental conditions was investigated. When preincubated with 500 mM glucose at 37°C for 20 hr, ALA-D was 80% inactivated and glycated hemoglobin levels were increased more than fourfold.Thiobarbituric acid species were not modified during glycation; therefore ALA-D inactivation cannot be attributed to glucose autoxidation.Acetyl salicylic acid was effective in preventing both hemoglobin glycation and ALA-D inactivation by glucose.A method has been developed for measuring protein glycation in vitro, in a crude preparation of red blood cells, which can also be applied to sugars other than glucose. Copyright (C) 1998 Elsevier Science Inc.

Registro:

Documento: Artículo
Título:Reducing sugars trigger δ-aminolevulinic dehydratase inactivation evidence of in vitro aspirin prevention
Autor:Caballero, F.A.; Gerez, E.N.; Polo, C.F.; Vazquez, E.S.; Del C. Batlle, A.M.
Filiación:Ctro. de Invest. sobre P., (CONICET - FCEN, UBA), Ciudad Universitaria, Pabellón II, 1428 Buenos Aires, Argentina
Viamonte 1881 10 A, 1056 Buenos Aires, Argentina
Palabras clave:δ-aminolevulinic dehydratase; Acetyl salicylic acid; Carbohydrates; Enzyme inactivation; Glycated hemoglobin; Glycation; Lipid peroxidation; acetylsalicylic acid; carbohydrate; glycosylated hemoglobin; porphobilinogen synthase; thiobarbituric acid; article; enzyme activity; enzyme inactivation; erythrocyte; human; human cell; lipid peroxidation; priority journal; protein glycosylation; temperature; Aspirin; Carbohydrate Metabolism; Carbohydrates; Glucose; Glycosylation; Hemoglobin A, Glycosylated; Humans; Lipid Peroxidation; Porphobilinogen Synthase; Temperature; Time Factors
Año:1998
Volumen:31
Número:3
Página de inicio:441
Página de fin:445
DOI: http://dx.doi.org/10.1016/S0306-3623(98)00016-0
Título revista:General Pharmacology
Título revista abreviado:Gen. Pharmacol.
ISSN:03063623
CODEN:GEPHD
CAS:Aspirin, 50-78-2; Carbohydrates; Glucose, 50-99-7; Hemoglobin A, Glycosylated; Porphobilinogen Synthase, EC 4.2.1.24
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v31_n3_p441_Caballero

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Citas:

---------- APA ----------
Caballero, F.A., Gerez, E.N., Polo, C.F., Vazquez, E.S. & Del C. Batlle, A.M. (1998) . Reducing sugars trigger δ-aminolevulinic dehydratase inactivation evidence of in vitro aspirin prevention. General Pharmacology, 31(3), 441-445.
http://dx.doi.org/10.1016/S0306-3623(98)00016-0
---------- CHICAGO ----------
Caballero, F.A., Gerez, E.N., Polo, C.F., Vazquez, E.S., Del C. Batlle, A.M. "Reducing sugars trigger δ-aminolevulinic dehydratase inactivation evidence of in vitro aspirin prevention" . General Pharmacology 31, no. 3 (1998) : 441-445.
http://dx.doi.org/10.1016/S0306-3623(98)00016-0
---------- MLA ----------
Caballero, F.A., Gerez, E.N., Polo, C.F., Vazquez, E.S., Del C. Batlle, A.M. "Reducing sugars trigger δ-aminolevulinic dehydratase inactivation evidence of in vitro aspirin prevention" . General Pharmacology, vol. 31, no. 3, 1998, pp. 441-445.
http://dx.doi.org/10.1016/S0306-3623(98)00016-0
---------- VANCOUVER ----------
Caballero, F.A., Gerez, E.N., Polo, C.F., Vazquez, E.S., Del C. Batlle, A.M. Reducing sugars trigger δ-aminolevulinic dehydratase inactivation evidence of in vitro aspirin prevention. Gen. Pharmacol. 1998;31(3):441-445.
http://dx.doi.org/10.1016/S0306-3623(98)00016-0