Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

1. δ-aminolevulinic acid (ALA) uptake as well as precursor accumulation and porphyrin biosynthesis were investigated in rat cerebellum, using as experimental approach minimal tissue units called particles. 2. ALA was shown to be taken up into cerebellum particles by a non saturable process up to 4.0 mM ALA whereas PBG and porphyrin formation exhibited a hyperbolic response reaching the plateau at about 1.0 and 1.5 mM ALA respectively. 3. Exposure of cerebellum particles to high exogenous ALA amounts (0.01-4.0 mM) indicated that ALA can be accumulated in relatively high concentrations in the cells (40 nmol/mg protein). Under these experimental conditions, PBG-D presented a low activity (3.25 pmol/mg protein/4 hr) showing to be a secondary control step in heme biosynthesis. 4. Incubation of cerebellum particles in the presence of a physiological concentration of glucose revealed that 1.0 mM ALA decreased glucose uptake by the cells (87% during 1 hr incubation), being consistent with the fact that acute attacks are precipitated by fasting and that sugar administration appeared to be an efficient treatment of AIP crisis. 5. These findings provide the basis for a useful model to study the nature of the metabolic mechanism underlying the acute attack. © 1994.

Registro:

Documento: Artículo
Título:Porphyrinogenesis in rat cerebellum. Effect of high δ-aminolevulinic acid concentration
Autor:Princ, F.G.; Juknat, A.A.; Del Carmen Batlle, A.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), (CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Viamonte 1881 10A 1056 CP-Buenos Aires, Argentina [Fax: 54 1 811 7447]
Palabras clave:porphyria; Porphyrinogenesis; rat cerebellum; δ-aminolevulinic acid; aminolevulinic acid; glucose; porphobilinogen; porphobilinogen deaminase; porphyrin; animal tissue; article; cerebellum; controlled study; drug accumulation; glucose transport; male; nonhuman; porphyria; porphyrin metabolism; priority journal; rat; Aminolevulinic Acid; Animal; Cerebellum; Glucose; Male; Porphyrins; Rats; Support, Non-U.S. Gov't
Año:1994
Volumen:25
Número:4
Página de inicio:761
Página de fin:766
DOI: http://dx.doi.org/10.1016/0306-3623(94)90257-7
Título revista:General Pharmacology
Título revista abreviado:Gen. Pharmacol. Vasc. Syst.
ISSN:03063623
CODEN:GEPHD
CAS:Aminolevulinic Acid, 106-60-5; Glucose, 50-99-7; Porphyrins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v25_n4_p761_Princ

Referencias:

  • Alperin, Idoyaga-Vargas, Carminatti, Rate of protein glycosylation in rat cerebral cortex (1986) J. Neurochem., 47, pp. 355-362
  • Becker, Viljoen, Kramer, The inhibition of red cell and brain ATPase by δ-aminolevulinic acid (1971) Biochim. biophys. Acta, 225, pp. 26-34
  • Becker, Kramer, Viljoen, Delta-aminolevulinic acid uptake by rabbit brain cerebral cortex (1974) J. Neurochem., 23, pp. 1019-1023
  • Becker, Viljoen, Kramer, Porphyrin precursors and their effects in vitro on some aspects of nerve function (1976) Porphyrins in human disease. First International Porphyrin Meeting, pp. 163-172. , M. Doss, Karger, Basel
  • Bonkowsky, Schady, Neurologic manifestations of acute porphyria (1982) Semin. Liver Dis., 2, pp. 108-124
  • Brennan, Cantrill, δ-Aminolevulinic acid is a potent agonist for GABA autoreceptors (1979) Nature, 280, pp. 514-515
  • Cutler, Moore, Ewart, Effects of delta-aminolevulinic acid administration on social behaviour in the laboratory mouse (1979) Psychopharmacology, 61, pp. 131-135
  • De Matteis, Ray, Studies on cerebellar haem metabolism in the rat in vivo (1982) J. Neurochem., 39, pp. 551-556
  • Durko, Juhasz, In vitro distribution of porphyrin metabolites from 10−3 M delta-aminolevulinic acid in primary neural tissue cultures (1987) Neurochemical Research, 12, pp. 465-468
  • Elder, Acquired disorders of haem synthesis (1976) Essays Med. Biochem., 2, pp. 75-114
  • Hermes-Lima, Valle, Vercesi, Bechara, Damage to rat liver mitochondria promoted by δ-aminolevulinic acid-generated reactive oxygen species: connections with acute intermittent porphyria and lead-poisoning (1991) Biochim. Biophys. Acta, 1056, pp. 57-63
  • Hermes-Lima, Castilho, Valle, Bechara, Vercesi, Calcium-dependent mitochondrial oxidative damage promoted by 5-aminolevulinic acid (1992) Biochim. biophys. Acta, 1180, pp. 201-206
  • Kotler, Juknat, Correa Garcia, Batlle, The action of sulphamerazine on porphyrin biosynthesis in rats (1988) Med. Sci. Res., 16, pp. 323-324
  • Lowry, Rosebrough, Farr, Randall, Protein measurement with the folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275
  • Maines, Regional distribution of the enzymes of haem biosynthesis and the inhibition of 5-aminolevulinate synthase by manganese in the rat brain (1980) Biochem. J., 190, pp. 315-321
  • Mauzerall, Granick, The occurrence and determination of δ-aminolevulinic acid and porphobilinogen in urine (1956) J. biol. Chem., 219, pp. 435-446
  • Percy, Shanley, Studies on haem biosynthesis in rat brain (1979) J. Neurochem., 33, pp. 1267-1274
  • Percy, Lamm, Taljaard, δ-aminolevulinic acid uptake, toxicity and effect on [14C]aminobutiric acid uptake into neurons and glia in culture (1981) J. Neurochem., 36, pp. 69-76
  • Russell, Lamm, Taljaard, Effects of δ-aminolevulinic acid, porphobilinogen and structurally related amino acids on 2-deoxy-glucose uptake in cultured neurons (1982) Neurochem. Res., 7, pp. 1009-1022
  • Russell, Lamm, Taljaard, Inhibition of Na+, K+-ATPase activity by δ-aminolevulinic acid (1983) Neurochem. Res., 8, pp. 1407-1415
  • Sima, Kennedy, Blakeslee, Robertson, Experimental porphyric neuropathy: a preliminary report (1981) Can. J. Neurol. Sci., 8, pp. 105-114
  • Sokoloff, Relation between physiological function and energy metabolism in the central nervous system (1977) J. Neurochem., 29, pp. 13-26
  • Sweeney, Pathak, Asbury, Acute intermittent porphyria (1970) Increased ALA-synthetase activity during an acute attack, 93, pp. 369-380. , Brain
  • Trinder, Colorimetric method for glucose determination in blood and other biological fluids (1969) Annals of Clinical Biochemistry: An international journal of biochemistry and laboratory medicine, 6, p. 24
  • Wetterberg, Report on an international survey of safe and unsafe drugs in acute and intermittent porphyria (1976) Porphyrins in Human Diseases—Report of Discussions, pp. 191-202. , M. Doss, P. Nawrocki, Falk, Freiburg
  • Whetsell, Sassa, Bickers, Kappas, Studies on porphyrin-heme biosynthesis in organotypic cultures of chick dorsal root ganglion (1978) J. Neuropath. exp. Neurol., 37, pp. 497-507
  • Yeung Laiwah, Goldberg, Moore, Pathogenesis and treatment of acute intermittent porphyria: discussion paper (1983) J. R. Soc. Med., 76, pp. 386-392
  • Yeung Laiwah, Moore, Goldberg, Pathogenesis of acute porphyria (1987) Q. J. Med., 63, pp. 377-392

Citas:

---------- APA ----------
Princ, F.G., Juknat, A.A. & Del Carmen Batlle, A.M. (1994) . Porphyrinogenesis in rat cerebellum. Effect of high δ-aminolevulinic acid concentration. General Pharmacology, 25(4), 761-766.
http://dx.doi.org/10.1016/0306-3623(94)90257-7
---------- CHICAGO ----------
Princ, F.G., Juknat, A.A., Del Carmen Batlle, A.M. "Porphyrinogenesis in rat cerebellum. Effect of high δ-aminolevulinic acid concentration" . General Pharmacology 25, no. 4 (1994) : 761-766.
http://dx.doi.org/10.1016/0306-3623(94)90257-7
---------- MLA ----------
Princ, F.G., Juknat, A.A., Del Carmen Batlle, A.M. "Porphyrinogenesis in rat cerebellum. Effect of high δ-aminolevulinic acid concentration" . General Pharmacology, vol. 25, no. 4, 1994, pp. 761-766.
http://dx.doi.org/10.1016/0306-3623(94)90257-7
---------- VANCOUVER ----------
Princ, F.G., Juknat, A.A., Del Carmen Batlle, A.M. Porphyrinogenesis in rat cerebellum. Effect of high δ-aminolevulinic acid concentration. Gen. Pharmacol. Vasc. Syst. 1994;25(4):761-766.
http://dx.doi.org/10.1016/0306-3623(94)90257-7