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Abstract:

1. 1. Some in vitro studies were performed to elucidate the action of S-adenosyl-l-methionine (SAM) and thiosulphate on liver rhodanese, δ-amino-levulinic acid dehydratase (ALA-D) and cytochrome oxidase affected by cyanide in the experimental conditions. 2. 2. SAM was unable to interact with the sulfur substituted rhodanese complex suggesting that SAM would blockade the thiosulphate binding sites on rhodanese. 3. 3. Cyanide and thiosulphate inhibited ALA-D activity when both compounds were present in the incubation or the preincubation mixture. Cyanide binding on the enzyme was irreversible. 4. 4. Cyanide inhibited cytochrome oxidase activity and the reversible nature of the binding was demonstrated by gel filtration. 5. 5. SAM had no effect on either ALA-D or cytochrome oxidase activities. © 1991.

Registro:

Documento: Artículo
Título:In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes
Autor:Buzaleh, A.M.; Vazquez, E.S.; Del Carmen Batlle, A.M.
Filiación:Centro de Investigaciones sobre Profirinas, Porfirias (CIPYP)-CONICET-Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Calle Viamonte 444/, 430 Buenos Aires, Argentina
Palabras clave:cyanide; cytochrome c oxidase; porphobilinogen synthase; s adenosylmethionine; thiosulfate; thiosulfate sulfurtransferase; animal tissue; article; controlled study; enzyme activity; liver homogenate; mouse; nonhuman; priority journal; Animal; Cyanides; Cytochrome-c Oxidase; In Vitro; Liver; Mice; Porphobilinogen Synthase; S-Adenosylmethionine; Support, Non-U.S. Gov't; Thiosulfate Sulfurtransferase; Thiosulfates
Año:1991
Volumen:22
Número:2
Página de inicio:281
Página de fin:286
DOI: http://dx.doi.org/10.1016/0306-3623(91)90449-G
Título revista:General Pharmacology
Título revista abreviado:Gen. Pharmacol. Vasc. Syst.
ISSN:03063623
CODEN:GEPHD
CAS:cyanide, 57-12-5; cytochrome c oxidase, 72841-18-0, 9001-16-5; porphobilinogen synthase, 9036-37-7; s adenosylmethionine, 29908-03-0, 485-80-3; thiosulfate sulfurtransferase, 9026-04-4; thiosulfate, 14383-50-7; Cyanides; Cytochrome-c Oxidase, EC 1.9.3.1; Porphobilinogen Synthase, EC 4.2.1.24; S-Adenosylmethionine, 29908-03-0; Thiosulfate Sulfurtransferase, EC 2.8.1.1; Thiosulfates
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v22_n2_p281_Buzaleh

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Citas:

---------- APA ----------
Buzaleh, A.M., Vazquez, E.S. & Del Carmen Batlle, A.M. (1991) . In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes. General Pharmacology, 22(2), 281-286.
http://dx.doi.org/10.1016/0306-3623(91)90449-G
---------- CHICAGO ----------
Buzaleh, A.M., Vazquez, E.S., Del Carmen Batlle, A.M. "In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes" . General Pharmacology 22, no. 2 (1991) : 281-286.
http://dx.doi.org/10.1016/0306-3623(91)90449-G
---------- MLA ----------
Buzaleh, A.M., Vazquez, E.S., Del Carmen Batlle, A.M. "In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes" . General Pharmacology, vol. 22, no. 2, 1991, pp. 281-286.
http://dx.doi.org/10.1016/0306-3623(91)90449-G
---------- VANCOUVER ----------
Buzaleh, A.M., Vazquez, E.S., Del Carmen Batlle, A.M. In vitro effect of cyanide, thiosulphate and S-adenosyl-l-methionine on the activity of rhodanese and other enzymes. Gen. Pharmacol. Vasc. Syst. 1991;22(2):281-286.
http://dx.doi.org/10.1016/0306-3623(91)90449-G