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Abstract:

1. 1. Results of studies on the kinetics of hepatic rhodanese and the effects of S-adenosyl-l-methionine (SAM) on these kinetic parameters in cyanide-treated and non-treated mice are reported here. 2. 2. The enzyme exhibited typical Michaelis-Menten behaviour with cyanide inhibition at concentrations higher than 50 mM. Km values of 4.74 and 0.85 mM were obtained for thiosulphate and cyanide, respectively, in control mice. 3. 3. These results stress the biological importance of the rhodanese reaction for cyanide detoxification. 4. 4. Km values were not significantly modified when the animals were intoxicated with a lethal (20 mg/kg) or a non-lethal (4 mg/kg) dose of cyanide. 5. 5. SAM treatment either in control or in cyanide-poisoned animals doubled the Km's for cyanide. © 1990.

Registro:

Documento: Artículo
Título:The effect of cyanide intoxication on hepatic rhodanese kinetics
Autor:Buzaleh, A.M.; Vazquez, E.S.; Alcira Maria, D.C.B.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:liver enzyme; potassium cyanide; s adenosylmethionine; animal cell; animal experiment; article; enzyme kinetics; intoxication; intraperitoneal drug administration; mouse; nonhuman; priority journal; xenobiotic metabolism; Animal; Cyanides; Kinetics; Liver; Mice; S-Adenosylmethionine; Sulfurtransferases; Support, Non-U.S. Gov't; Thiosulfate Sulfurtransferase; Thiosulfates
Año:1990
Volumen:21
Número:2
Página de inicio:219
Página de fin:222
DOI: http://dx.doi.org/10.1016/0306-3623(90)90904-Z
Título revista:General Pharmacology
Título revista abreviado:Gen. Pharmacol. Vasc. Syst.
ISSN:03063623
CODEN:GEPHD
CAS:potassium cyanide, 151-50-8; s adenosylmethionine, 29908-03-0, 485-80-3; Cyanides; S-Adenosylmethionine, 29908-03-0; Sulfurtransferases, EC 2.8.1; Thiosulfate Sulfurtransferase, EC 2.8.1.1; Thiosulfates
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03063623_v21_n2_p219_Buzaleh

Referencias:

  • Anosike, Jack, Kidney rhodanese from the guinea pig (Lepus caniculus) and albino rat (Mus musculus). (1982) Enzyme, 27, pp. 33-39
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  • Saunders, Himwich, Properties of the transsulfurase responsible for conversion of cyanide to thiocyanate. (1950) Am J Physiol, 163, pp. 404-409
  • Sörbo, Rhodanese (1955) Methods in Enzymology, pp. 334-335. , S.P. Colowick, N.O. Kaplan, Academic Press, New York
  • van Rensburg, Schabort, Rhodanese from Cercopithecus aethiops (vervet monkey) liver—II. Aspects of enzyme kinetics and mechanism of action (1984) International Journal of Biochemistry, 16 (5), pp. 547-551
  • Vázquez, Buzaleh, Wider, Batlle, Reduced thiosulphate affinity for erythrocyte rhodanese: an explanation for increased δ-aminolevulinic synthetase activity in some porphyrias? (1986) IRCS Med. Sci., 14, pp. 999-1000
  • Vázquez, Buzaleh, Wider, Batlle, Soluble and immobilized RP. palustris rhodanese—II. Some particular kinetic behaviour (1987) International Journal of Biochemistry, 19, pp. 1199-1203
  • Watanabe, Nakano, Kitaoka, Subcellular location and some properties of rhodanese in Euglena gracilis. (1985) Agricultural and Biological Chemistry, 49 (7), pp. 2203-2204
  • Westley, Rhodanese. (1973) Adv Enzymol Relat Areas Mol Biol, 39, pp. 327-368
  • Westley, Heyse, Mechanisms of sulfur transfer catalysis. Sulfhydryl-catalyzed transfer of thiosulfonate sulfur. (1971) J Biol Chem, 246, pp. 1468-1474

Citas:

---------- APA ----------
Buzaleh, A.M., Vazquez, E.S. & Alcira Maria, D.C.B. (1990) . The effect of cyanide intoxication on hepatic rhodanese kinetics. General Pharmacology, 21(2), 219-222.
http://dx.doi.org/10.1016/0306-3623(90)90904-Z
---------- CHICAGO ----------
Buzaleh, A.M., Vazquez, E.S., Alcira Maria, D.C.B. "The effect of cyanide intoxication on hepatic rhodanese kinetics" . General Pharmacology 21, no. 2 (1990) : 219-222.
http://dx.doi.org/10.1016/0306-3623(90)90904-Z
---------- MLA ----------
Buzaleh, A.M., Vazquez, E.S., Alcira Maria, D.C.B. "The effect of cyanide intoxication on hepatic rhodanese kinetics" . General Pharmacology, vol. 21, no. 2, 1990, pp. 219-222.
http://dx.doi.org/10.1016/0306-3623(90)90904-Z
---------- VANCOUVER ----------
Buzaleh, A.M., Vazquez, E.S., Alcira Maria, D.C.B. The effect of cyanide intoxication on hepatic rhodanese kinetics. Gen. Pharmacol. Vasc. Syst. 1990;21(2):219-222.
http://dx.doi.org/10.1016/0306-3623(90)90904-Z