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Abstract:

Background and Aims In mature quinoa (Chenopodium quinoa) seeds, the lasting endosperm forms a micropylar cone covering the radicle. The suspensor cells lie within the centre of the cone. During the final stage of seed development, the cells of the lasting endosperm accumulate protein and lipids while the rest are crushed and disintegrated. Both the suspensor and endosperm die progressively from the innermost layers surrounding the embryo and extending towards the nucellar tissue. Ricinosomes are endoplasmic reticulum-derived organelles that accumulate both the pro-form and the mature form of cysteine endopeptidase (Cys-EP), first identified in castor bean (Ricinus communis) endosperm during germination. This study sought to identify associations between the presence of ricinosomes and programmed cell death (PCD) hallmarks in suspensor and endosperm cells predestined to die during quinoa seed development. MethodsAstructural study using light microscopy and transmission electron microscopywas performed. To detect the presence of Cys-EP, both western blot and in situ immunolocalization assays were carried out using anti- R. communis Cys-EP antibody. ATUNEL assay was used to determine DNA fragmentation. Results and Conclusions Except for the one or two cell layers that constitute the lasting endosperm in the mature seed, ricinosomes were found in suspensor and endosperm cells. These cells were also the site of morphological abnormalities, including misshapen and fragmented nuclei, vesiculation of the cytosol, vacuole collapse and cell wall disorganization. It is proposed that, in suspensor and endosperm cells, the early detection of Cys-EP in ricinosomes predicts the occurrence of PCD during late seed development. © The Author 2013.

Registro:

Documento: Artículo
Título:Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)
Autor:López-Fernández, M.P.; Maldonado, S.
Filiación:Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Intendente Guiraldes 2160 Pab. 2, C1428EGA, Argentina
Palabras clave:Amaranthaceae; Chenopodiaceae; Chenopodium quinoa; Cys-EP; Cys-EP in situ immunolocalization; Cys-EP western blot; Cysteine endopeptidase; Endosperm; Micropylar endosperm; PCD; Programmed cell death; Ricinosomes; Suspensor; TUNEL.; abnormality; bioassay; biological development; DNA; enzyme activity; germination; immune response; lipid; maturation; protein; seed; sperm; cysteine proteinase; Amaranthaceae; apoptosis; article; cell death; cell fractionation; cell nucleus; cell organelle; Chenopodium quinoa; Cys-EP; Cys-EP in situ immunolocalization; Cys-EP western blot; cytology; DNA fragmentation; endosperm; enzymology; flow cytometry; growth, development and aging; metabolism; micropylar endosperm; nick end labeling; PCD; ricinosomes; suspensor; ultrastructure; Amaranthaceae; Chenopodiaceae; Chenopodium quinoa; Cys-EP; Cys-EP in situ immunolocalization; Cys-EP western blot; cysteine endopeptidase; endosperm; micropylar endosperm; PCD; programmed cell death; ricinosomes; suspensor; TUNEL; Cell Death; Cell Nucleus; Chenopodium quinoa; Cysteine Endopeptidases; DNA Fragmentation; Endosperm; Flow Cytometry; Organelles; Subcellular Fractions
Año:2013
Volumen:112
Número:7
Página de inicio:1253
Página de fin:1262
DOI: http://dx.doi.org/10.1093/aob/mct184
Título revista:Annals of Botany
Título revista abreviado:Ann. Bot.
ISSN:03057364
CODEN:ANBOA
CAS:cysteine proteinase, 37353-41-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057364_v112_n7_p1253_LopezFernandez

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Citas:

---------- APA ----------
López-Fernández, M.P. & Maldonado, S. (2013) . Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa). Annals of Botany, 112(7), 1253-1262.
http://dx.doi.org/10.1093/aob/mct184
---------- CHICAGO ----------
López-Fernández, M.P., Maldonado, S. "Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)" . Annals of Botany 112, no. 7 (2013) : 1253-1262.
http://dx.doi.org/10.1093/aob/mct184
---------- MLA ----------
López-Fernández, M.P., Maldonado, S. "Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa)" . Annals of Botany, vol. 112, no. 7, 2013, pp. 1253-1262.
http://dx.doi.org/10.1093/aob/mct184
---------- VANCOUVER ----------
López-Fernández, M.P., Maldonado, S. Ricinosomes provide an early indicator of suspensor and endosperm cells destined to die during late seed development in quinoa (Chenopodium quinoa). Ann. Bot. 2013;112(7):1253-1262.
http://dx.doi.org/10.1093/aob/mct184