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Abstract:

Background and AimsPre-harvest sprouting susceptibility in grain sorghum (Sorghum bicolor) is related to low seed dormancy and reduced embryo sensitivity to inhibition of germination by abscisic acid (ABA). Intra-specific variability for pre-harvest sprouting might involve differential regulation of ABA signalling genes.MethodsSorghum genes encoding homologues for ABA signalling components from other species (ABI5, ABI4, VP1, ABI1 and PKABA1) were studied at the transcriptional and protein level (ABI5) during grain imbibition for two sorghum lines with contrasting sprouting phenotypes and in response to hormones.Key ResultsTranscript levels of these genes and protein levels of ABI5 were higher in imbibed immature caryopses of the more dormant line. Dormancy loss was related to lower transcript levels of these genes and lower ABI5 protein levels in both genotypes. Exogenous ABA inhibited germination of isolated embryos but failed to prevent ABI5 rapid decrease supporting a role for the seed coat in regulating ABI5 levels.ConclusionsSeveral genes involved in ABA signalling are regulated differently in imbibed caryopses from two sorghum lines with contrasting pre-harvest sprouting response before - but not after - physiological maturity. A role for ABI5 in the expression of dormancy during grain development is discussed.

Registro:

Documento: Artículo
Título:Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages
Autor:Rodríguez, M.V.; Mendiondo, G.M.; Maskin, L.; Gudesblat, G.E.; Iusem, N.D.; Benech-Arnold, R.L.
Filiación:IFEVA, CONICET-UBA, UBA, Av. San Martín 4453, C1417DSE, Buenos Aires, Argentina
LFBM, FCEyN, UBA, Pabellón II, Ciudad Universitaria, 1428, Buenos Aires, Argentina
VIB Department of Plant Systems Biology, Ghent University, Technologiepark 927, 9052 Gent, Belgium
Palabras clave:ABI5; Abscisic acid; Hormone signalling; Pre-harvest sprouting; Seed dormancy; Sorghum bicolor; abscisic acid; phytohormone; signal transducing adaptor protein; vegetable protein; abscisic acid; developmental stage; embryo; gene expression; harvesting; hormone; imbibition; inhibition; intraspecific variation; protein; seed dormancy; sorghum; article; gene expression regulation; genetics; germination; growth, development and aging; physiology; plant gene; plant seed; polymerase chain reaction; sorghum; Western blotting; Abscisic Acid; Adaptor Proteins, Signal Transducing; Blotting, Western; Gene Expression Regulation, Plant; Genes, Plant; Germination; Plant Growth Regulators; Plant Proteins; Polymerase Chain Reaction; Seeds; Sorghum; Sorghum bicolor; Sorghum bicolor bicolor
Año:2009
Volumen:104
Número:5
Página de inicio:975
Página de fin:985
DOI: http://dx.doi.org/10.1093/aob/mcp184
Handle:http://hdl.handle.net/20.500.12110/paper_03057364_v104_n5_p975_Rodriguez
Título revista:Annals of Botany
Título revista abreviado:Ann. Bot.
ISSN:03057364
CODEN:ANBOA
CAS:abscisic acid, 21293-29-8; Abscisic Acid, 21293-29-8; Adaptor Proteins, Signal Transducing; Plant Growth Regulators; Plant Proteins
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03057364_v104_n5_p975_Rodriguez.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03057364_v104_n5_p975_Rodriguez

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Citas:

---------- APA ----------
Rodríguez, M.V., Mendiondo, G.M., Maskin, L., Gudesblat, G.E., Iusem, N.D. & Benech-Arnold, R.L. (2009) . Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages. Annals of Botany, 104(5), 975-985.
http://dx.doi.org/10.1093/aob/mcp184
---------- CHICAGO ----------
Rodríguez, M.V., Mendiondo, G.M., Maskin, L., Gudesblat, G.E., Iusem, N.D., Benech-Arnold, R.L. "Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages" . Annals of Botany 104, no. 5 (2009) : 975-985.
http://dx.doi.org/10.1093/aob/mcp184
---------- MLA ----------
Rodríguez, M.V., Mendiondo, G.M., Maskin, L., Gudesblat, G.E., Iusem, N.D., Benech-Arnold, R.L. "Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages" . Annals of Botany, vol. 104, no. 5, 2009, pp. 975-985.
http://dx.doi.org/10.1093/aob/mcp184
---------- VANCOUVER ----------
Rodríguez, M.V., Mendiondo, G.M., Maskin, L., Gudesblat, G.E., Iusem, N.D., Benech-Arnold, R.L. Expression of ABA signalling genes and ABI5 protein levels in imbibed Sorghum bicolor caryopses with contrasting dormancy and at different developmental stages. Ann. Bot. 2009;104(5):975-985.
http://dx.doi.org/10.1093/aob/mcp184