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Bertolin, A.P.; Katz, M.J.; Yano, M.; Pozzi, B.; Acevedo, J.M.; Blanco-Obregón, D.; Gándara, L.; Sorianello, E.; Kanda, H.; Okano, H.; Srebrow, A.; Wappner, P. "Musashi mediates translational repression of the Drosophila hypoxia inducible factor" (2016) Nucleic Acids Research. 44(16):7555-7567
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Abstract:

Adaptation to hypoxia depends on a conserved α/β heterodimeric transcription factor called Hypoxia Inducible Factor (HIF), whose α-subunit is regulated by oxygen through different concurrent mechanisms. In this study, we have identified the RNA binding protein dMusashi, as a negative regulator of the fly HIF homologue Sima. Genetic interaction assays suggested that dMusashi participates of the HIF pathway, and molecular studies carried out in Drosophila cell cultures showed that dMusashi recognizes a Musashi Binding Element in the 3′ UTR of the HIFα transcript, thereby mediating its translational repression in normoxia. In hypoxic conditions dMusashi is downregulated, lifting HIFα repression and contributing to trigger HIF-dependent gene expression. Analysis performed in mouse brains revealed that murine Msi1 protein physically interacts with HIF-1α transcript, suggesting that the regulation of HIF by Msi might be conserved in mammalian systems. Thus, Musashi is a novel regulator of HIF that inhibits responses to hypoxia specifically when oxygen is available. © 2016 The Author(s). Published by Oxford University Press on behalf of Nucleic Acids Research.

Registro:

Documento: Artículo
Título:Musashi mediates translational repression of the Drosophila hypoxia inducible factor
Autor:Bertolin, A.P.; Katz, M.J.; Yano, M.; Pozzi, B.; Acevedo, J.M.; Blanco-Obregón, D.; Gándara, L.; Sorianello, E.; Kanda, H.; Okano, H.; Srebrow, A.; Wappner, P.
Filiación:Instituto Leloir, Patricias Argentinas 435, Buenos Aires, 1405, Argentina
Division of Neurobiology and Anatomy, Graduate School of Medical and Dental Sciences, Niigata University, 1-757, Asahimachidori, Chuo-ku, Niigata, Niigata, 951-8510, Japan
Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE, UBA-CONICET), Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EHA, Argentina
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EHA, Argentina
Department of Physiology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, 160-8582, Japan
German Cancer Research Center (DKFZ), Heidelberg, Germany
Palabras clave:hypoxia inducible factor; Musashi protein; Musashi1 protein; RNA binding protein; unclassified drug; DNA binding protein; Drosophila protein; hypoxia inducible factor 1alpha; messenger RNA; musashi protein, Drosophila; protein binding; RNA binding protein; Sima protein, Drosophila; 3' untranslated region; animal cell; animal tissue; Article; cell hypoxia; controlled study; down regulation; Drosophila melanogaster; embryo; gene expression; gene interaction; insect cell culture; mouse; nonhuman; priority journal; protein protein interaction; translation regulation; animal; biological model; embryology; gene locus; genetic transcription; genetics; growth, development and aging; mammal; metabolism; nucleotide sequence; protein synthesis; signal transduction; trachea; Animals; Base Sequence; DNA-Binding Proteins; Down-Regulation; Drosophila melanogaster; Drosophila Proteins; Genetic Loci; Hypoxia-Inducible Factor 1, alpha Subunit; Mammals; Models, Biological; Protein Binding; Protein Biosynthesis; RNA, Messenger; RNA-Binding Proteins; Signal Transduction; Trachea; Transcription, Genetic
Año:2016
Volumen:44
Número:16
Página de inicio:7555
Página de fin:7567
DOI: http://dx.doi.org/10.1093/nar/gkw372
Título revista:Nucleic Acids Research
Título revista abreviado:Nucleic Acids Res.
ISSN:03051048
CODEN:NARHA
CAS:DNA-Binding Proteins; Drosophila Proteins; Hypoxia-Inducible Factor 1, alpha Subunit; musashi protein, Drosophila; RNA, Messenger; RNA-Binding Proteins; Sima protein, Drosophila
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v44_n16_p7555_Bertolin

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Citas:

---------- APA ----------
Bertolin, A.P., Katz, M.J., Yano, M., Pozzi, B., Acevedo, J.M., Blanco-Obregón, D., Gándara, L.,..., Wappner, P. (2016) . Musashi mediates translational repression of the Drosophila hypoxia inducible factor. Nucleic Acids Research, 44(16), 7555-7567.
http://dx.doi.org/10.1093/nar/gkw372
---------- CHICAGO ----------
Bertolin, A.P., Katz, M.J., Yano, M., Pozzi, B., Acevedo, J.M., Blanco-Obregón, D., et al. "Musashi mediates translational repression of the Drosophila hypoxia inducible factor" . Nucleic Acids Research 44, no. 16 (2016) : 7555-7567.
http://dx.doi.org/10.1093/nar/gkw372
---------- MLA ----------
Bertolin, A.P., Katz, M.J., Yano, M., Pozzi, B., Acevedo, J.M., Blanco-Obregón, D., et al. "Musashi mediates translational repression of the Drosophila hypoxia inducible factor" . Nucleic Acids Research, vol. 44, no. 16, 2016, pp. 7555-7567.
http://dx.doi.org/10.1093/nar/gkw372
---------- VANCOUVER ----------
Bertolin, A.P., Katz, M.J., Yano, M., Pozzi, B., Acevedo, J.M., Blanco-Obregón, D., et al. Musashi mediates translational repression of the Drosophila hypoxia inducible factor. Nucleic Acids Res. 2016;44(16):7555-7567.
http://dx.doi.org/10.1093/nar/gkw372