Artículo

Palm, T.; Hemmer, K.; Winter, J.; Fricke, I.B.; Tarbashevich, K.; Sadeghi Shakib, F.; Rudolph, I.-M.; Hillje, A.-L.; De Luca, P.; Bahnassawy, L.; Madel, R.; Viel, T.; De Siervi, A.; Jacobs, A.H.; Diederichs, S.; Schwamborn, J.C. "A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop" (2013) Nucleic Acids Research. 41(6):3699-3712
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Abstract:

Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and miRNA profiling and isolated functional interaction networks of involved mechanisms. Thereby, we identified an E2F1-miRNA feedback loop as important regulator of NSC fate decisions. Although E2F1 supports NSC proliferation and represses transcription of miRNAs from the miR-17∼92 and miR-106a∼363 clusters, these miRNAs are transiently up-regulated at early stages of neuronal differentiation. In these early committed cells, increased miRNAs expression levels directly repress E2F1 mRNA levels and inhibit cellular proliferation. In mice, we demonstrated that these miRNAs are expressed in the neurogenic areas and that E2F1 inhibition represses NSC proliferation. The here presented data suggest a novel interaction mechanism between E2F1 and miR-17∼92 / miR-106a∼363 miRNAs in controlling NSC proliferation and neuronal differentiation. © The Author(s) 2013. Published by Oxford University Press.

Registro:

Documento: Artículo
Título:A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop
Autor:Palm, T.; Hemmer, K.; Winter, J.; Fricke, I.B.; Tarbashevich, K.; Sadeghi Shakib, F.; Rudolph, I.-M.; Hillje, A.-L.; De Luca, P.; Bahnassawy, L.; Madel, R.; Viel, T.; De Siervi, A.; Jacobs, A.H.; Diederichs, S.; Schwamborn, J.C.
Filiación:Westfälische Wilhelms-Universität Münster, ZMBE, Stem Cell Biology and Regeneration Group, 48149 Münster, Germany
Helmholtz-University-Group Molecular RNA Biology and Cancer, German Cancer Research Center (DKFZ), 69120 Heidelberg, Germany
University of Heidelberg, Institute of Pathology, 69120 Heidelberg, Germany
Westfälische Wilhelms-Universität Münster, European Institute for Molecular Imaging (EIMI), 48149 Münster, Germany
Interdisciplinary Center for Clinical Research (IZKF), 48149 Münster, Germany
Westfälische Wilhelms-Universität Münster, ZMBE, Institute of Cell Biology, 48149 Münster, Germany
University of Buenos Aires, Department of Biological Chemistry, School of Sciences, C1428EGA Buenos Aires, Argentina
Palabras clave:messenger RNA; microRNA; microRNA 106a; microRNA 17; microRNA 363; microRNA 92; transcription factor E2F1; unclassified drug; animal cell; article; cell fate; cell proliferation; controlled study; feedback system; gene cluster; gene expression profiling; genetic transfection; growth inhibition; mouse; nerve cell differentiation; neural stem cell; nonhuman; priority journal; protein analysis; protein function; protein RNA binding; regulatory mechanism; transcription termination; transcriptomics; upregulation; Animals; Cell Cycle; Cells, Cultured; E2F1 Transcription Factor; Feedback, Physiological; Gene Expression Profiling; Gene Expression Regulation; Mice; MicroRNAs; Neural Stem Cells; Neurogenesis; RNA, Messenger; Mus
Año:2013
Volumen:41
Número:6
Página de inicio:3699
Página de fin:3712
DOI: http://dx.doi.org/10.1093/nar/gkt070
Título revista:Nucleic Acids Research
Título revista abreviado:Nucleic Acids Res.
ISSN:03051048
CODEN:NARHA
CAS:E2F1 Transcription Factor; E2f1 protein, mouse; MIRN17-92 microRNA, mouse; MicroRNAs; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v41_n6_p3699_Palm

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Citas:

---------- APA ----------
Palm, T., Hemmer, K., Winter, J., Fricke, I.B., Tarbashevich, K., Sadeghi Shakib, F., Rudolph, I.-M.,..., Schwamborn, J.C. (2013) . A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop. Nucleic Acids Research, 41(6), 3699-3712.
http://dx.doi.org/10.1093/nar/gkt070
---------- CHICAGO ----------
Palm, T., Hemmer, K., Winter, J., Fricke, I.B., Tarbashevich, K., Sadeghi Shakib, F., et al. "A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop" . Nucleic Acids Research 41, no. 6 (2013) : 3699-3712.
http://dx.doi.org/10.1093/nar/gkt070
---------- MLA ----------
Palm, T., Hemmer, K., Winter, J., Fricke, I.B., Tarbashevich, K., Sadeghi Shakib, F., et al. "A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop" . Nucleic Acids Research, vol. 41, no. 6, 2013, pp. 3699-3712.
http://dx.doi.org/10.1093/nar/gkt070
---------- VANCOUVER ----------
Palm, T., Hemmer, K., Winter, J., Fricke, I.B., Tarbashevich, K., Sadeghi Shakib, F., et al. A systemic transcriptome analysis reveals the regulation of neural stem cell maintenance by an E2F1-miRNA feedback loop. Nucleic Acids Res. 2013;41(6):3699-3712.
http://dx.doi.org/10.1093/nar/gkt070