Artículo

Bertucci, P.Y.; Nacht, A.S.; Alló, M.; Rocha-Viegas, L.; Ballaré, C.; Soronellas, D.; Castellano, G.; Zaurin, R.; Kornblihtt, A.R.; Beato, M.; Vicent, G.P.; Pecci, A. "Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation" (2013) Nucleic Acids Research. 41(12):6072-6086
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Abstract:

Steroid receptors were classically described for regulating transcription by binding to target gene promoters. However, genome-wide studies reveal that steroid receptors-binding sites are mainly located at intragenic regions. To determine the role of these sites, we examined the effect of progestins on the transcription of the bcl-x gene, where only intragenic progesterone receptor-binding sites (PRbs) were identified. We found that in response to hormone treatment, the PR is recruited to these sites along with two histone acetyltransferases CREB-binding protein (CBP) and GCN5, leading to an increase in histone H3 and H4 acetylation and to the binding of the SWI/SNF complex. Concomitant, a more relaxed chromatin was detected along bcl-x gene mainly in the regions surrounding the intragenic PRbs. PR also mediated the recruitment of the positive elongation factor pTEFb, favoring RNA polymerase II (Pol II) elongation activity. Together these events promoted the re-distribution of the active Pol II toward the 3′-end of the gene and a decrease in the ratio between proximal and distal transcription. These results suggest a novel mechanism by which PR regulates gene expression by facilitating the proper passage of the polymerase along hormone-dependent genes. © 2013 The Author(s) 2013. Published by Oxford University Press.

Registro:

Documento: Artículo
Título:Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation
Autor:Bertucci, P.Y.; Nacht, A.S.; Alló, M.; Rocha-Viegas, L.; Ballaré, C.; Soronellas, D.; Castellano, G.; Zaurin, R.; Kornblihtt, A.R.; Beato, M.; Vicent, G.P.; Pecci, A.
Filiación:IFIBYNE-CONICET, Buenos Aires C1428EGA, Argentina
Gene Regulation Program, Centre for Genomic Regulation (CRG), Barcelona E-08003, Spain
University Pompeu Fabra (UPF), Barcelona E-08003, Spain
Departamento de Fisiología y Biología Molecular y Celular, FCEN-UBA, Buenos Aires C1428EGA, Argentina
Departamento de Química Biológica, FCEN-UBA, Buenos Aires C1428EGA, Argentina
Palabras clave:cyclic AMP responsive element binding protein binding protein; histone acetyltransferase; histone acetyltransferase GCN5; histone H3; histone H4; messenger RNA; progesterone receptor; protein bcl x; protein SWI; RNA polymerase II; transcription factor SNF; 3' untranslated region; alternative RNA splicing; article; Bcl x gene; binding site; chromatin assembly and disassembly; complex formation; controlled study; exon; gene expression regulation; gene function; gene induction; gene location; gene targeting; histone acetylation; human; human cell; intron; priority journal; transcription elongation; transcription initiation; Alternative Splicing; bcl-X Protein; Binding Sites; Cell Line, Tumor; Chromatin; CREB-Binding Protein; Humans; p300-CBP Transcription Factors; Positive Transcriptional Elongation Factor B; Promegestone; Receptors, Progesterone; RNA Polymerase II; Transcription Elongation, Genetic
Año:2013
Volumen:41
Número:12
Página de inicio:6072
Página de fin:6086
DOI: http://dx.doi.org/10.1093/nar/gkt327
Título revista:Nucleic Acids Research
Título revista abreviado:Nucleic Acids Res.
ISSN:03051048
CODEN:NARHA
CAS:cyclic AMP responsive element binding protein binding protein, 190209-80-4; histone acetyltransferase, 9054-51-7; CREB-Binding Protein, 2.3.1.48; CREBBP protein, human; Chromatin; Positive Transcriptional Elongation Factor B, 2.7.11.-; Promegestone, 34184-77-5; RNA Polymerase II, 2.7.7.-; Receptors, Progesterone; bcl-X Protein; p300-CBP Transcription Factors, 2.3.1.48; p300-CBP-associated factor, 2.3.1.48
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v41_n12_p6072_Bertucci

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Citas:

---------- APA ----------
Bertucci, P.Y., Nacht, A.S., Alló, M., Rocha-Viegas, L., Ballaré, C., Soronellas, D., Castellano, G.,..., Pecci, A. (2013) . Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation. Nucleic Acids Research, 41(12), 6072-6086.
http://dx.doi.org/10.1093/nar/gkt327
---------- CHICAGO ----------
Bertucci, P.Y., Nacht, A.S., Alló, M., Rocha-Viegas, L., Ballaré, C., Soronellas, D., et al. "Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation" . Nucleic Acids Research 41, no. 12 (2013) : 6072-6086.
http://dx.doi.org/10.1093/nar/gkt327
---------- MLA ----------
Bertucci, P.Y., Nacht, A.S., Alló, M., Rocha-Viegas, L., Ballaré, C., Soronellas, D., et al. "Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation" . Nucleic Acids Research, vol. 41, no. 12, 2013, pp. 6072-6086.
http://dx.doi.org/10.1093/nar/gkt327
---------- VANCOUVER ----------
Bertucci, P.Y., Nacht, A.S., Alló, M., Rocha-Viegas, L., Ballaré, C., Soronellas, D., et al. Progesterone receptor induces bcl-x expression through intragenic binding sites favoring RNA polymerase II elongation. Nucleic Acids Res. 2013;41(12):6072-6086.
http://dx.doi.org/10.1093/nar/gkt327