Abstract:
Removal of the poly(A) tail is the first step in the degradation of many eukaryotic mRNAs. In metazoans and yeast, the Ccr4/Caf1/Not complex has the predominant deadenylase activity, while the Pan2/Pan3 complex may trim poly(A) tails to the correct size, or initiate deadenylation. In trypanosomes, turnover of several constitutively-expressed or long-lived mRNAs is not affected by depletion of the 5'-3' exoribonuclease XRNA, but is almost completely inhibited by depletion of the deadenylase CAF1. In contrast, two highly unstable mRNAs, encoding EP procyclin and a phosphoglycerate kinase, PGKB, accumulate when XRNA levels are reduced. We here show that degradation of EP mRNA was partially inhibited after CAF1 depletion. RNAi-targeting trypanosome PAN2 had a mild effect on global deadenylation, and on degradation of a few mRNAs including EP. By amplifying and sequencing degradation intermediates, we demonstrated that a reduction in XRNA had no effect on degradation of a stable mRNA encoding a ribosomal protein, but caused accumulation of EP mRNA fragments that had lost substantial portions of the 5' and 3' ends. The results support a model in which trypanosome mRNAs can be degraded by at least two different, partially independent, cytoplasmic degradation pathways attacking both ends of the mRNA. © 2009 The Author(s).
Registro:
Documento: |
Artículo
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Título: | The role of deadenylation in the degradation of unstable mRNAs in trypanosomes |
Autor: | Schwede, A.; Manful, T.; Jha, B.A.; Helbig, C.; Bercovich, N.; Stewart, M.; Clayton, C. |
Filiación: | Zentrum für Molekulare Biologie (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany Instituto de Investigaciones en Ingeniería, Genética y Biología Molecular (INGEBI), FCEyN, Dto. FBMC, Universidad de Buenos Aires, Vuelta de Obligado 2490 2P, 1428, Buenos Aires, Argentina Glasgow Biomedical Research Centre, University of Glasgow, G12 8QQ, United Kingdom
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Palabras clave: | chromatin assembly factor 1; gene product; messenger RNA; protein EP; ribosome protein; unclassified drug; EP procyclin protein, Trypanosoma brucei; membrane protein; mRNA deadenylase; phosphoglycerate kinase; protozoal protein; ribonuclease; 3' untranslated region; 5' untranslated region; adenylation; article; controlled study; cytoplasm; gene amplification; gene sequence; nonhuman; priority journal; protein depletion; protein targeting; RNA degradation; RNA interference; Trypanosoma; animal; chemistry; drug antagonism; enzymology; genetics; genome; growth, development and aging; metabolism; physiology; RNA stability; Eukaryota; Metazoa; Animals; Cytoplasm; Genome, Protozoan; Membrane Glycoproteins; Phosphoglycerate Kinase; Protozoan Proteins; Ribonucleases; RNA Interference; RNA Stability; RNA, Messenger; Trypanosoma |
Año: | 2009
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Volumen: | 37
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Número: | 16
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Página de inicio: | 5511
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Página de fin: | 5528
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DOI: |
http://dx.doi.org/10.1093/nar/gkp571 |
Título revista: | Nucleic Acids Research
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Título revista abreviado: | Nucleic Acids Res.
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ISSN: | 03051048
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CODEN: | NARHA
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CAS: | phosphoglycerate kinase, 9001-83-6; ribonuclease, 59794-03-5, 9001-99-4; EP procyclin protein, Trypanosoma brucei; Membrane Glycoproteins; Phosphoglycerate Kinase, 2.7.2.3; Protozoan Proteins; RNA, Messenger; Ribonucleases, 3.1.-; mRNA deadenylase, 3.1.-
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PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_03051048_v37_n16_p5511_Schwede.pdf |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v37_n16_p5511_Schwede |
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Citas:
---------- APA ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M. & Clayton, C.
(2009)
. The role of deadenylation in the degradation of unstable mRNAs in trypanosomes. Nucleic Acids Research, 37(16), 5511-5528.
http://dx.doi.org/10.1093/nar/gkp571---------- CHICAGO ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M., et al.
"The role of deadenylation in the degradation of unstable mRNAs in trypanosomes"
. Nucleic Acids Research 37, no. 16
(2009) : 5511-5528.
http://dx.doi.org/10.1093/nar/gkp571---------- MLA ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M., et al.
"The role of deadenylation in the degradation of unstable mRNAs in trypanosomes"
. Nucleic Acids Research, vol. 37, no. 16, 2009, pp. 5511-5528.
http://dx.doi.org/10.1093/nar/gkp571---------- VANCOUVER ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M., et al. The role of deadenylation in the degradation of unstable mRNAs in trypanosomes. Nucleic Acids Res. 2009;37(16):5511-5528.
http://dx.doi.org/10.1093/nar/gkp571