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Removal of the poly(A) tail is the first step in the degradation of many eukaryotic mRNAs. In metazoans and yeast, the Ccr4/Caf1/Not complex has the predominant deadenylase activity, while the Pan2/Pan3 complex may trim poly(A) tails to the correct size, or initiate deadenylation. In trypanosomes, turnover of several constitutively-expressed or long-lived mRNAs is not affected by depletion of the 5'-3' exoribonuclease XRNA, but is almost completely inhibited by depletion of the deadenylase CAF1. In contrast, two highly unstable mRNAs, encoding EP procyclin and a phosphoglycerate kinase, PGKB, accumulate when XRNA levels are reduced. We here show that degradation of EP mRNA was partially inhibited after CAF1 depletion. RNAi-targeting trypanosome PAN2 had a mild effect on global deadenylation, and on degradation of a few mRNAs including EP. By amplifying and sequencing degradation intermediates, we demonstrated that a reduction in XRNA had no effect on degradation of a stable mRNA encoding a ribosomal protein, but caused accumulation of EP mRNA fragments that had lost substantial portions of the 5' and 3' ends. The results support a model in which trypanosome mRNAs can be degraded by at least two different, partially independent, cytoplasmic degradation pathways attacking both ends of the mRNA. © 2009 The Author(s).


Documento: Artículo
Título:The role of deadenylation in the degradation of unstable mRNAs in trypanosomes
Autor:Schwede, A.; Manful, T.; Jha, B.A.; Helbig, C.; Bercovich, N.; Stewart, M.; Clayton, C.
Filiación:Zentrum für Molekulare Biologie (ZMBH), DKFZ-ZMBH Alliance, Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany
Instituto de Investigaciones en Ingeniería, Genética y Biología Molecular (INGEBI), FCEyN, Dto. FBMC, Universidad de Buenos Aires, Vuelta de Obligado 2490 2P, 1428, Buenos Aires, Argentina
Glasgow Biomedical Research Centre, University of Glasgow, G12 8QQ, United Kingdom
Palabras clave:chromatin assembly factor 1; gene product; messenger RNA; protein EP; ribosome protein; unclassified drug; EP procyclin protein, Trypanosoma brucei; membrane protein; mRNA deadenylase; phosphoglycerate kinase; protozoal protein; ribonuclease; 3' untranslated region; 5' untranslated region; adenylation; article; controlled study; cytoplasm; gene amplification; gene sequence; nonhuman; priority journal; protein depletion; protein targeting; RNA degradation; RNA interference; Trypanosoma; animal; chemistry; drug antagonism; enzymology; genetics; genome; growth, development and aging; metabolism; physiology; RNA stability; Eukaryota; Metazoa; Animals; Cytoplasm; Genome, Protozoan; Membrane Glycoproteins; Phosphoglycerate Kinase; Protozoan Proteins; Ribonucleases; RNA Interference; RNA Stability; RNA, Messenger; Trypanosoma
Página de inicio:5511
Página de fin:5528
Título revista:Nucleic Acids Research
Título revista abreviado:Nucleic Acids Res.
CAS:phosphoglycerate kinase, 9001-83-6; ribonuclease, 59794-03-5, 9001-99-4; EP procyclin protein, Trypanosoma brucei; Membrane Glycoproteins; Phosphoglycerate Kinase,; Protozoan Proteins; RNA, Messenger; Ribonucleases, 3.1.-; mRNA deadenylase, 3.1.-


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---------- APA ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M. & Clayton, C. (2009) . The role of deadenylation in the degradation of unstable mRNAs in trypanosomes. Nucleic Acids Research, 37(16), 5511-5528.
---------- CHICAGO ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M., et al. "The role of deadenylation in the degradation of unstable mRNAs in trypanosomes" . Nucleic Acids Research 37, no. 16 (2009) : 5511-5528.
---------- MLA ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M., et al. "The role of deadenylation in the degradation of unstable mRNAs in trypanosomes" . Nucleic Acids Research, vol. 37, no. 16, 2009, pp. 5511-5528.
---------- VANCOUVER ----------
Schwede, A., Manful, T., Jha, B.A., Helbig, C., Bercovich, N., Stewart, M., et al. The role of deadenylation in the degradation of unstable mRNAs in trypanosomes. Nucleic Acids Res. 2009;37(16):5511-5528.