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Abstract:

The major satellite DNA of the subterranean rodent Ctenomys, named RPCS, contains several consensus sequences characteristic of the U3 region of retroviral long terminal repeats (LTRs), such as a polypurine tract, CCAAT boxes, binding sites for the CC-AAT/enhancer-blnding protein (C/EBP), a TATA box and putative polyadenylatlon signals. RPCS presents an enormous variation in abundance between species of the same genus: while C.australls or C.talarum have approximately 3×106 copies per genome, C.opimus has none. A sequence (RPCS-I) with Identity to the SV40-enhancer core element, present In all the repeating units of the satellite is specifically protected In DNase I footprlntings. Competitions of band-shift assays with different transcription factor binding sites Indicate that binding to RPCS-I is specific and involves CCAAT proteins related to NF-1, but not to C/EBP. By the use of quantitative protein/DNA binding assays we determined that, despite of their conspicuous difference in RPCS copy number, C.talarum and C.opimus have equivalent amounts and Identical quality of RPCS-bindlng proteins. These results are consistent with the observation, by in situ hybridization, that RPCS is clustered in heterochromatic regions, where it might have restricted accessibility to transcription factors in vivo. This is the first report of the binding of transcription factors to a satellite DNA of retroviral origin. © 1994 Oxford University Press.

Registro:

Documento: Artículo
Título:Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys
Autor:Pesce, C.G.; Rossi, M.S.; Muro, A.F.; Reig, O.A.; Zorzópulos, J.; Kornblihtt, A.R.
Filiación:DInstitute de Investigaciones en Ingenien'a Genética y Biologi'a Molecular (INGEBI-CONICET), Buenos Aires and Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Vuelta de Obligado 2490, 2o Piso, Argentina
Grupo de Investigaciones en Biologi'a Evolutiva (GIBE), Departamento de Ciencias Biológicas, Argentina
Departamento de Qufmica Biológica, F.C.E.y N., U.B.A., Argentina
Palabras clave:binding protein; deoxyribonuclease i; nuclear factor; nuclear factor i; purine; satellite dna; transcription factor; animal cell; animal tissue; article; binding site; consensus sequence; controlled study; dna binding; dna footprinting; enhancer region; genetic variability; genome; in situ hybridization; long terminal repeat; nonhuman; polyadenylation; priority journal; protein binding; retrovirus; rodent; simian virus 40; tata box; Animals; Base Sequence; DNA, Satellite; DNA, Viral; DNA-Binding Proteins; Enhancer Elements (Genetics); Genome; Molecular Sequence Data; Nuclear Proteins; Retroviridae; Rodentia; Sensitivity and Specificity; Animalia; Ctenomys; Rodentia; Simiae; Simian virus; Simian virus 40; unidentified retrovirus
Año:1994
Volumen:22
Número:4
Página de inicio:656
Página de fin:661
DOI: http://dx.doi.org/10.1093/nar/22.4.656
Título revista:Nucleic Acids Research
Título revista abreviado:Nucleic Acids Res.
ISSN:03051048
CODEN:NARHA
CAS:deoxyribonuclease I, 9003-98-9; purine, 120-73-0; DNA, Satellite; DNA, Viral; DNA-Binding Proteins; Nuclear Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03051048_v22_n4_p656_Pesce

Referencias:

  • Reig, O.A., Busch, C., Ortells, M.O., Contreras, J., (1990) Evolutionary Biology of Subterranean Mammals, pp. 71-96. , Nevo, E. and Reig, O. A. (Alan R. Liss, NY)
  • Rossi, M.S., Reig, O.A., Zorzópulos, J., (1990) Mol. Biol. Evol., 7, pp. 340-350
  • Rossi, M.S., Reig, O.A., Zorzópulos, J., (1993) Int. J. Mamm. Biol., 58, pp. 244-251
  • Rossi, M.S., Pesce, C.G., Reig, O.A., Kornblihtt, A.R., Zorzpulos, J., (1993) DNA Seq., 3, pp. 379-382
  • Gorski, K., Cameiro, M., Schibier, U., (1986) Cell., 47, pp. 767-776
  • Digram, J.D., Lebowitz, R.M., Roeder, R.G., (1983) Nucleic Acids Res., 11, pp. 1475-1489
  • Muro, A.F., Bernath, V.A., Kornblihtt, A.R., (1992) J. Biol. Chem., 267, pp. 12767-12774
  • Wuarin, J., Schibier, U., (1990) Cell., 63, pp. 1257-1266
  • Raymondjean, M., Cereghini, S., Yaniv, M., (1988) Proc. Natl. Acad. Sci. USA., 85, pp. 757-761
  • Bakker, O., Parker, M.G., (1991) Nucleic Acids Res., 19, pp. 1213-1217
  • Galas, D., Schmitz, R., (1978) Nucleic Acids Res., 5, pp. 3157-3170
  • Bernath, V.A., Muro, A.F., Vitullo, A.D., Bley, M.A., Baraûao, J.L., Kornblihtt, A.R., (1990) J. Biol. Chem., 265, pp. 18219-18226
  • Chodosh, L.A., Carthew, L.W., Sharp, P.A., (1986) Mol. Cell. Biol., 6, pp. 4723-4733
  • Hübscher, U., (1987) Nucleic Acids Res., 15, pp. 5486-5491
  • Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual., , (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY)
  • Sanger, F., Nicklen, S., Coulson, A.R., (1977) Proc. Natl. Acad. Sci. USA., 74, pp. 5463-5467
  • Maxam, A., Gilbert, W., (1980) Meth. Enzymol., 65, pp. 499-560
  • Barros, M.A., Patton, J.L., (1985) Chromosoma., 92, pp. 337-343
  • Lee, A., Elder, F., (1980) Cytogent. Cell Genet., 26, pp. 36-40
  • Pardue, M.L., (1985) Nucleic Acids Hybridization., pp. 179-202. , Flames, B. D. and Higgins, S. J. (IRL Press, Oxford)
  • Burns, J., Chan, V.T.-W., Joansson, J.A., Fleming, K.A., Taylor, S., Gee, J.O.D., (1985) J. Clin. Pathol., 38, pp. 1085-1092
  • Ryden, T.A., Beemon, K., (1989) Mol. Cell. Biol., 9, pp. 1155-1164
  • Massarini, A.I., Barros, M.A., Ortells, M.O., Reig, O.A., (1991) Genetica., 83, pp. 131-144
  • Doolittle, W.F., Sapienza, C., (1980) Nature, 284, pp. 601-603
  • John, T.C., Miklos, G.L.C., (1986) Int. Rev. Cytol., 58, pp. 1-114
  • Brosius, J., Gould, S.J., (1992) Proc. Natl. Acad. Sci. USA., 89, pp. 10706-10710
  • Dover, G., (1982) Nature, 299, pp. 111-117
  • Doshi, P., Kaushal, S., Benyajati, C., Wu, C.-I., (1991) Mol. Biol. Evol., 8, pp. 721-741
  • Haaf, T., Warburton, P.E., Willard, H.F., (1992) Cell., 70, pp. 681-696

Citas:

---------- APA ----------
Pesce, C.G., Rossi, M.S., Muro, A.F., Reig, O.A., Zorzópulos, J. & Kornblihtt, A.R. (1994) . Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys. Nucleic Acids Research, 22(4), 656-661.
http://dx.doi.org/10.1093/nar/22.4.656
---------- CHICAGO ----------
Pesce, C.G., Rossi, M.S., Muro, A.F., Reig, O.A., Zorzópulos, J., Kornblihtt, A.R. "Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys" . Nucleic Acids Research 22, no. 4 (1994) : 656-661.
http://dx.doi.org/10.1093/nar/22.4.656
---------- MLA ----------
Pesce, C.G., Rossi, M.S., Muro, A.F., Reig, O.A., Zorzópulos, J., Kornblihtt, A.R. "Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys" . Nucleic Acids Research, vol. 22, no. 4, 1994, pp. 656-661.
http://dx.doi.org/10.1093/nar/22.4.656
---------- VANCOUVER ----------
Pesce, C.G., Rossi, M.S., Muro, A.F., Reig, O.A., Zorzópulos, J., Kornblihtt, A.R. Binding of nuclear factors to a satellite DNA of retroviral origin with marked differences in copy number among species of the rodent Ctenomys. Nucleic Acids Res. 1994;22(4):656-661.
http://dx.doi.org/10.1093/nar/22.4.656