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Navone, N.M.; Polo, C.F.; Frisardi, A.L.; M. del C. Batlle, A. "Mouse mammary carcinoma porphobilinogenase and hydroxymethylbilane synthetase" (1991) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 98(1):67-71
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Abstract:

1. 1. Porphobilinogenase (PBGase) and hydroxymethylbilane synthetase (HMB-S) were investigated in crude extracts from mouse mammary carcinoma, normal mouse liver and tumor bearing mouse liver. 2. 2. A Michaelis-Menten kinetics for both enzymes in either source was observed. Km values of 87 to 108 μM, Vmax of 1.57-1.83 nmol porphyrins/2 hr and a Hill coefficient of n = 1 were obtained for PBGase and Km values of 13 to 19 μM and Vmax of 2.6-4.8 were obtained for HMB-S. 3. 3. Porphyrin synthesis was linear up to 180 min of incubation in all cases for PBGase and HMB-S, and greatly increased with higher incubation temperature being maximal at 60°C and nil at 70°C, optimal temperature was 37°C for either enzyme in either source. 4. 4. Uroporphyrinogen III synthetase was heat inactivated while HMB-S was a heat stable enzyme. Optimum pH was 8.2 for PBGase and HMB-S in either tissue. © 1991.

Registro:

Documento: Artículo
Título:Mouse mammary carcinoma porphobilinogenase and hydroxymethylbilane synthetase
Autor:Navone, N.M.; Polo, C.F.; Frisardi, A.L.; M. del C. Batlle, A.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET-FCEN, UBA, Ciudad Universitaria, Pabellon II, 2do Piso, 1428 Buenos Aires, Argentina
Palabras clave:porphobilinogen deaminase; porphobilinogenase; unclassified drug; uroporphyrinogen iii synthase; animal tissue; article; breast adenocarcinoma; liver; male; mouse; nonhuman; priority journal; Ammonia-Lyases; Animal; Hydrogen-Ion Concentration; Hydroxymethylbilane Synthase; Kinetics; Liver; Male; Mammary Neoplasms, Experimental; Mice; Mice, Inbred BALB C; Neoplasm Transplantation; Porphyrins; Support, Non-U.S. Gov't; Temperature; Animalia
Año:1991
Volumen:98
Número:1
Página de inicio:67
Página de fin:71
DOI: http://dx.doi.org/10.1016/0305-0491(91)90309-2
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
CAS:Ammonia-Lyases, EC 4.3.1.; Hydroxymethylbilane Synthase, EC 4.3.1.8; porphobilinogenase, EC 5.-; Porphyrins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v98_n1_p67_Navone

Referencias:

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  • Battersby, Fookes, Matcham, Pandey, Biosynthesis of porphyrisn and related macrocycle Part 21 The interaction of deaminase and its products (hydroxymethylbilane) and the relationship between deaminase and cosynthetase (1983) Journal of the Chemical Society, Perkin Transactions 1, 1, pp. 3041-3047
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Citas:

---------- APA ----------
Navone, N.M., Polo, C.F., Frisardi, A.L. & M. del C. Batlle, A. (1991) . Mouse mammary carcinoma porphobilinogenase and hydroxymethylbilane synthetase. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 98(1), 67-71.
http://dx.doi.org/10.1016/0305-0491(91)90309-2
---------- CHICAGO ----------
Navone, N.M., Polo, C.F., Frisardi, A.L., M. del C. Batlle, A. "Mouse mammary carcinoma porphobilinogenase and hydroxymethylbilane synthetase" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 98, no. 1 (1991) : 67-71.
http://dx.doi.org/10.1016/0305-0491(91)90309-2
---------- MLA ----------
Navone, N.M., Polo, C.F., Frisardi, A.L., M. del C. Batlle, A. "Mouse mammary carcinoma porphobilinogenase and hydroxymethylbilane synthetase" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 98, no. 1, 1991, pp. 67-71.
http://dx.doi.org/10.1016/0305-0491(91)90309-2
---------- VANCOUVER ----------
Navone, N.M., Polo, C.F., Frisardi, A.L., M. del C. Batlle, A. Mouse mammary carcinoma porphobilinogenase and hydroxymethylbilane synthetase. 1991;98(1):67-71.
http://dx.doi.org/10.1016/0305-0491(91)90309-2