Navegar

Colección

Datos Estadísticas

Artículo

Paveto, C.; Passeron, S.; Stella, A.M.; Batle, A.M.d.C. "Regulatory role of δ-aminolevulic acid dehydratase in the dimorphic fungus Mucor rouxii" (1989) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 94(4):635-639
La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

1. 1. With the aim of finding a possible relationship between the known dimorphism phenomenon existing in the fungus Mucor rouxii and the biosynthesis of respiratory pigments, the activity of aminolevulic acid synthetase (ALA-S) and ALA-dehydratase (ALA-D) was studied in crude extracts and in 15,000 g supernatants of both mycelium and yeast-like cells. 2. 2. The activity of ALA-S was unusually high (3 nmol ALA/hr/mg protein) compared with that reported for other tissues and did not vary with the fungus morphology. 3. 3. Instead, ALA-D specific activity was found to be 16.5 nmol PBG/hr/mg protein in mycelium extracts, that is 7-fold greater than that measured in the yeast-like morphology (2.6 nmol PBG/hr/mg protein). 4. 4. It was of importance to determine the activity levels of ALA-D along with the morphogenic transition from yeast to mycelium. It was observed that the greatest change and enhancing of specific activity occurred 2 hr before the emergence of the germ tubes and was held constant up to the complete development of mycelium. 5. 5. Both hyphae formation and enhancement of ALA-D activity were diminished when cAMP was added to the culture shifted from the anaerobic atmosphere to air. 6. 6. These findings and preliminary studies on the characterization of M. rouxii ALA-D indicate that this enzyme plays a regulatory role in porphyrin biosynthesis in this fungus as well as a key function in the characteristic morphogenic transition. © 1989.

Registro:

Documento: Artículo
Título:Regulatory role of δ-aminolevulic acid dehydratase in the dimorphic fungus Mucor rouxii
Autor:Paveto, C.; Passeron, S.; Stella, A.M.; Batle, A.M.d.C.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Año:1989
Volumen:94
Número:4
Página de inicio:635
Página de fin:639
DOI: http://dx.doi.org/10.1016/0305-0491(89)90141-7
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v94_n4_p635_Paveto

Referencias:

  • de, 5-Aminolaevulinate hydro-lyase from yeast (1967) Isolation and Purification, 139, pp. 479-486. , Biochim. biophys. Acta
  • Barralho, Panek, Malamud, Sanders, Mattoon, In situ assay for 5-aminolevulinate dehydratase and application to the study of a catabolite repression-resistant Saccharomyces cerevisiae mutant (1983) J. Bact., 156, pp. 141-147
  • Bartnicki-García, Nickerson, Induction of yeast-like development in Mucor by carbon dioxide (1962) J Bacteriol, 84, pp. 829-840
  • Batlle, Ferramola, Grinstein, Purification and general properties of delta aminolaevulic acid dehydratase from cow liver (1967) Biochem. J., 104, pp. 244-249
  • Beale, Castelfranco, The biosynthesis of δ-aminolevulinic acid in higher plants II. Formation of 14C-δ-aminolevulinic acid from labeled precursors (1974) PLANT PHYSIOLOGY, 53, pp. 297-303
  • Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding (1976) Analyt. Biochem., 72, pp. 248-254
  • Clark-Walker, Relationship between dimorphology and respiration in Mucor genevensis studied with chloramphenicol (1973) J. Bact., 116, pp. 972-980
  • Ebbon, Tait, Studies on δ-adenosyl methionine-magnesium protoporphyrin methyltransferase in Euglena gracilis. Strain Z (1969) Biochem. J., 111, pp. 573-578
  • Fukuda, Paredes, Batlle, Active site histidine in pig liver aminolevulic acid dehydratase modified by diethylpyrocarbonate and protected by Zn2+ ions (1988) Comp. Biochem. Physiol., 91 B, pp. 285-291
  • Ito, Chilar, Inderlied, Lipid synthesis during morphogenesis of Mucor racemosus (1982) J. Bact., 152, pp. 880-887
  • Jayaraman, Padmanaban, Malathi, Sarma, Heme synthesis during mitochondriogenesis in yeast (1971) Biochem. J., 121, pp. 531-535
  • Komai, Neilands, Effect of zinc ions on delta-ALA-dehydratase in Ustilago sphaerogena (1968) Archs Biochem. Biophys., 124, pp. 456-461
  • Labbe-Bois, Urban-Grimal, Volland, Camadro, Dehoux, About the regulation of protoheme synthesis in the yeast Saccharomyces cerevisiae (1983) Mitochondria, pp. 523-524
  • Labbe-Bois, Volland, Changes in the activities of the protoheme synthesis system during the growth of yeast under different conditions (1977) Archives of Biochemistry and Biophysics, 179, pp. 565-577
  • Larsen, Sypherd, Cyclic adenosine 3′-5′-monophosphate and morphogenesis in Mucor racemosus (1974) J. Bact., 117, pp. 432-438
  • Lascelles, (1964) Tetrapyrrole Biosynthesis and its Regulation, , W. A. Benhamin, New York
  • Menon, Shemin, Concurrent decrease of enzymic activities concerned with the synthesis of coenzyme B12 and of propionic acid in Propionebacteria (1967) Archives of Biochemistry and Biophysics, 121, pp. 304-309
  • Muthukrishnan, Malati, Padmanaban, δ-aminolaevulinate dehydratase, the regulatory enzyme of the haembiosynthetic pathway Neurospora crassa (1972) Biochem. J., 129, pp. 31-37
  • Nemeth, Russell, Shemin, The succinate-glycine cycle II. Metabolism of δ-aminolevulinic acid (1958) J. biol. Chem., 229, pp. 415-422
  • Paveto, Epstein, Passeron, Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxii (1975) Archs. Biochem. Biophys., 169, pp. 449-457
  • Paveto, Passeron, Studies on the phosphofructokinase activity of the dimorphic fungus Mucor rouxii (1977) Archs. Biochem. Biophys., 178, pp. 1-7
  • Paznokas, Sypherd, Pyruvate kinase isoenzymes of Mucor racemosus: control of synthesis by glucose (1977) J. Bact., 130, pp. 661-666
  • Sanders, Mied, Briquet, Hernandez, Rodriguez, Goetal, Mattoon, Regulation of mitochondrial biogenesis: yeast mutants deficient in synthesis of δ-aminolevulinic acid (1973) J. molec. Biol., 80, pp. 17-39
  • Stella, Estudios sobre la enzima delta aminolevúlico dehidratasa (1977) Ph.D. thesis, , University of Buenos Aires, Argentina
  • Viale, Biosíntesis de bacteriochlorofila en Rhodopseudomonas palustris - Sistemas enzimáticos reguladores (1978) Ph.D. thesis, , University of Buenos Aires, Argentina
  • Wider de Xifra, Tigier, Porphyrin biosynthesis in soybean callus tissue system VIII. Isolation, purification and general properties of Succinyl CoA Synthetase (1971) Enzymology, 41, pp. 217-231
  • Wider de Xifra, Batlle, Tigier, δ-aminolevulinate synthetase in extracts of cultured soybean cells (1971) Biochimica et Biophysica Acta (BBA) - Enzymology, 235, pp. 511-517

Citas:

---------- APA ----------
Paveto, C., Passeron, S., Stella, A.M. & Batle, A.M.d.C. (1989) . Regulatory role of δ-aminolevulic acid dehydratase in the dimorphic fungus Mucor rouxii. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 94(4), 635-639.
http://dx.doi.org/10.1016/0305-0491(89)90141-7
---------- CHICAGO ----------
Paveto, C., Passeron, S., Stella, A.M., Batle, A.M.d.C. "Regulatory role of δ-aminolevulic acid dehydratase in the dimorphic fungus Mucor rouxii" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 94, no. 4 (1989) : 635-639.
http://dx.doi.org/10.1016/0305-0491(89)90141-7
---------- MLA ----------
Paveto, C., Passeron, S., Stella, A.M., Batle, A.M.d.C. "Regulatory role of δ-aminolevulic acid dehydratase in the dimorphic fungus Mucor rouxii" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 94, no. 4, 1989, pp. 635-639.
http://dx.doi.org/10.1016/0305-0491(89)90141-7
---------- VANCOUVER ----------
Paveto, C., Passeron, S., Stella, A.M., Batle, A.M.d.C. Regulatory role of δ-aminolevulic acid dehydratase in the dimorphic fungus Mucor rouxii. 1989;94(4):635-639.
http://dx.doi.org/10.1016/0305-0491(89)90141-7