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Juknat, A.A.; Kotler, M.L.; Koopmann, G.E.; Batlle, A.M.d.C. "Porphobilinogenase from Rhodopseudomonas palustris" (1989) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 92(2):291-295
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Abstract:

1. 1. Porphobilinogenase (PBGase) from Rp. palustris has been isolated and some properties of a partially purified fraction were studied. 2. 2. PBGase has an optimum pH of 7.4 when activity was expressed in terms of porphyrins formed and two pH maxima at 7.4 and 8.5 when activity was based on the amount of PBG consumed. 3. 3. Cyclotetramerization rate and distribution of reaction products were not affected either by the presence or absence of oxygen. 4. 4. Two PBGase active species of mol. wt 115,000 and 50,000 were found, by means of gel filtration through a calibrated Sephadex G-100 column. 5. 5. Kinetic data show the existence of positive cooperative effects for porphyrin formation, while a hyperbolic behaviour for PBG consumption was observed. © 1989.

Registro:

Documento: Artículo
Título:Porphobilinogenase from Rhodopseudomonas palustris
Autor:Juknat, A.A.; Kotler, M.L.; Koopmann, G.E.; Batlle, A.M.d.C.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (FCEN, UBA-CONICET), Ciudad Universitaria, Pabellon II, 2do. Piso, 1428 Buenos Aires, Argentina
Palabras clave:ammonia lyase; Ammonia Lyases; porphobilinogenase; article; enzymology; gel chromatography; isolation and purification; kinetics; metabolism; molecular weight; pH; Rhodopseudomonas; Ammonia-Lyases; Chromatography, Gel; Hydrogen-Ion Concentration; Kinetics; Molecular Weight; Rhodopseudomonas; Support, Non-U.S. Gov't
Año:1989
Volumen:92
Número:2
Página de inicio:291
Página de fin:295
DOI: http://dx.doi.org/10.1016/0305-0491(89)90280-0
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
CAS:porphobilinogenase, 9055-40-7; Ammonia-Lyases, EC 4.3.1.; porphobilinogenase, EC 5.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v92_n2_p291_Juknat

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Citas:

---------- APA ----------
Juknat, A.A., Kotler, M.L., Koopmann, G.E. & Batlle, A.M.d.C. (1989) . Porphobilinogenase from Rhodopseudomonas palustris. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 92(2), 291-295.
http://dx.doi.org/10.1016/0305-0491(89)90280-0
---------- CHICAGO ----------
Juknat, A.A., Kotler, M.L., Koopmann, G.E., Batlle, A.M.d.C. "Porphobilinogenase from Rhodopseudomonas palustris" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 92, no. 2 (1989) : 291-295.
http://dx.doi.org/10.1016/0305-0491(89)90280-0
---------- MLA ----------
Juknat, A.A., Kotler, M.L., Koopmann, G.E., Batlle, A.M.d.C. "Porphobilinogenase from Rhodopseudomonas palustris" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 92, no. 2, 1989, pp. 291-295.
http://dx.doi.org/10.1016/0305-0491(89)90280-0
---------- VANCOUVER ----------
Juknat, A.A., Kotler, M.L., Koopmann, G.E., Batlle, A.M.d.C. Porphobilinogenase from Rhodopseudomonas palustris. 1989;92(2):291-295.
http://dx.doi.org/10.1016/0305-0491(89)90280-0