Artículo

Kotler, M.L.; Fumagalli, S.A.; Juknat, A.A.; del C. Batlle, A.M. "Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase" (1987) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 87(3):601-606
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Abstract:

1. 1. Uroporphyrinogen I synthetase from Rhodopseudomonas palustris has been isolated and purified. 2. 2. Assay conditions were determined. 3. 3. Sephadex G-100 column chromatography was found to increase 4-fold the degree of purification yielding a deaminase that was purified 72-fold. 4. 4. Some properties of the isolated enzyme were studied. The optimal pH was about 7.6-7.8. Porphyrin formation was linear with time. The presence of several thiol reagents was found to be no essential for deaminase activity. 5. 5. Deaminase exhibited classical Michaelis-Menten kinetics Km and Vmax were estimated. 6. 6. Molecular weight determinations, by means of gel filtration on a calibrated Sephadex G-100 column, gave values of 74,000 ± 7400. © 1987.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
Autor:Kotler, M.L.; Fumagalli, S.A.; Juknat, A.A.; del C. Batlle, A.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), (CONICET Facultad de Ciencias Exactas y Naturales, University of Buenos Aires), Ciudad Universitaria, Pabellon II 2 Piso, Buenos Aires, Argentine
Año:1987
Volumen:87
Número:3
Página de inicio:601
Página de fin:606
DOI: http://dx.doi.org/10.1016/0305-0491(87)90058-7
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p601_Kotler

Referencias:

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Citas:

---------- APA ----------
Kotler, M.L., Fumagalli, S.A., Juknat, A.A. & del C. Batlle, A.M. (1987) . Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 87(3), 601-606.
http://dx.doi.org/10.1016/0305-0491(87)90058-7
---------- CHICAGO ----------
Kotler, M.L., Fumagalli, S.A., Juknat, A.A., del C. Batlle, A.M. "Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 87, no. 3 (1987) : 601-606.
http://dx.doi.org/10.1016/0305-0491(87)90058-7
---------- MLA ----------
Kotler, M.L., Fumagalli, S.A., Juknat, A.A., del C. Batlle, A.M. "Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 87, no. 3, 1987, pp. 601-606.
http://dx.doi.org/10.1016/0305-0491(87)90058-7
---------- VANCOUVER ----------
Kotler, M.L., Fumagalli, S.A., Juknat, A.A., del C. Batlle, A.M. Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase. 1987;87(3):601-606.
http://dx.doi.org/10.1016/0305-0491(87)90058-7