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Rossetti, M.V.; Araujo, L.S.; Lombardo, M.E.; García, S.C.; del C. Batlle, A.M. "Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties" (1987) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 87(3):593-600
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Abstract:

1. 1. The effect of light and growing time on chlorophyll, protein content and the activity of porphobilinogenase (PBGase), in Euglena gracilis was studied. 2. 2. It was found that neither protein nor chlorophyll content or PBGase activity were dependent upon the days of growing or the light conditions of growth. 3. 3. Some properties of PBGase were studied. The addition of sodium and magnesium salts to the assay system did not modify enzyme activity. Uroporphyrinogen formation was linear with protein over a wide range of enzyme concentration. 4. 4. When activity was expressed on the basis of the amount of porphyrins formed an optimum pH of 7.4 was obtained but when it was expressed in terms of PBG consumed two pH maxima at 7.0 and 8.5 were observed. 5. 5. Direct plots of velocity against PBG concentration, when activity was measured as PBG consumed, were not hyperbolic; double-reciprocal plots were not linear and Hill plots gave a n value of 2, indicating the existence of positive co-operative effects between two binding sites for PBG per molecule of PBGase. © 1987.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties
Autor:Rossetti, M.V.; Araujo, L.S.; Lombardo, M.E.; García, S.C.; del C. Batlle, A.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), (CONICET-Facultad de Ciencias Exactas y Naturales, UBA) Ciudad Universitaria, Pabellón II 20 Piso, 1428 Buenos Aires Argentine, Argentina
Año:1987
Volumen:87
Número:3
Página de inicio:593
Página de fin:600
DOI: http://dx.doi.org/10.1016/0305-0491(87)90057-5
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v87_n3_p593_Rossetti

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Citas:

---------- APA ----------
Rossetti, M.V., Araujo, L.S., Lombardo, M.E., García, S.C. & del C. Batlle, A.M. (1987) . Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 87(3), 593-600.
http://dx.doi.org/10.1016/0305-0491(87)90057-5
---------- CHICAGO ----------
Rossetti, M.V., Araujo, L.S., Lombardo, M.E., García, S.C., del C. Batlle, A.M. "Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 87, no. 3 (1987) : 593-600.
http://dx.doi.org/10.1016/0305-0491(87)90057-5
---------- MLA ----------
Rossetti, M.V., Araujo, L.S., Lombardo, M.E., García, S.C., del C. Batlle, A.M. "Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 87, no. 3, 1987, pp. 593-600.
http://dx.doi.org/10.1016/0305-0491(87)90057-5
---------- VANCOUVER ----------
Rossetti, M.V., Araujo, L.S., Lombardo, M.E., García, S.C., del C. Batlle, A.M. Porphyrin biosynthesis in Euglena gracilis-VI. The effect of growth conditions on porphobilinogenase activity and further properties. 1987;87(3):593-600.
http://dx.doi.org/10.1016/0305-0491(87)90057-5