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Salzman, T.A.; Batlle, A.M.D.C.; de Souza, W. "Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium" (1986) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 83(1):57-61
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Abstract:

1. 1. The activity of the following enzymes involved in the biosynthesis of porphyrins was determined in two strains of Trypanosoma cruzi (Y and CL) grown in two culture media (LIT and Warren): succinyl coenzyme A synthetase (Suc.CoA-S), 5-aminolevulinate synthetase (ALA-S), 4,5-dioxovaleric acid transaminase (DOVA-T), 5-aminolevulinate dehydratase (ALA-D), porphobilinogenase (PBGase), deaminase and heme synthetase (Heme-S). The amount of 5-aminolevulinic acid (ALA) and porphobilinogen, porphyrins and heme was also determined. 2. 2. ALA and PGB were detected in both strains of T. cruzi. However, ALA was not detected in epimastigotes of the Y strain grown in the LIT medium. The content of ALA and PBG varied according to the strain and the growth medium. 3. 3. No free porphyrins and heme were detected in both strains of T. cruzi. 4. 4. The activity of Suc.CoA-S and DOVA-T was markedly influenced by the strains of the parasite and the growth medium. No significant DOVA-T activity was detected in epimastigotes of the CL strain grown in the Warren's medium. 5. 5. No significant activity of ALA-D, PBGase and deaminase was detected in T. cruzi. 6. 6. Activity of Heme-S was detected in both strains of T. cruzi when mesoporphyrin, protoporphyrin or deuteroporphyrin was used as substrate. The enzyme activity was influenced by the strain of the parasite, the growth medium and the substrate used. © 1986.

Registro:

Documento: Artículo
Título:Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium
Autor:Salzman, T.A.; Batlle, A.M.D.C.; de Souza, W.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias-CIPyP, FCEyN., Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
Instituto de Biofísica, Universidade Federal do Rio de Janeiro, Ilha do Fundão, 21941, Rio de Janeiro, Brasil (Tel: 280-8093)
Palabras clave:5 aminolevulinate synthase; aminolevulinate aminotransferase; aminotransferase; ammonia lyase; Ammonia Lyases; ferrochelatase; heme; porphobilinogen synthase; porphobilinogenase; Succinate CoA Ligases; succinyl coenzyme A synthetase; animal; article; biosynthesis; comparative study; culture medium; metabolism; species difference; Trypanosoma cruzi; 5-Aminolevulinate Synthetase; Ammonia-Lyases; Animal; Comparative Study; Culture Media; Ferrochelatase; Heme; Porphobilinogen Synthase; Species Specificity; Succinate-CoA Ligases; Support, Non-U.S. Gov't; Transaminases; Trypanosoma cruzi
Año:1986
Volumen:83
Número:1
Página de inicio:57
Página de fin:61
DOI: http://dx.doi.org/10.1016/0305-0491(86)90331-7
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
CAS:5 aminolevulinate synthase, 9037-14-3; aminolevulinate aminotransferase, 9012-46-8; aminotransferase, 9031-66-7; ferrochelatase, 9012-93-5; heme, 14875-96-8; porphobilinogen synthase, 9036-37-7; porphobilinogenase, 9055-40-7; succinyl coenzyme A synthetase, 37341-57-4, 9014-36-2, 9080-33-5; 5-Aminolevulinate Synthetase, EC 2.3.1.37; aminolevulinate aminotransferase, EC 2.6.1.43; Ammonia-Lyases, EC 4.3.1.; Culture Media; Ferrochelatase, EC 4.99.1.1; Heme, 14875-96-8; Porphobilinogen Synthase, EC 4.2.1.24; porphobilinogenase, EC 5.-; Succinate-CoA Ligases, EC 6.2.1.-; Transaminases, EC 2.6.1.
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v83_n1_p57_Salzman

Referencias:

  • Alcantara, Brener, The in vitro interaction of Trypanosoma cruzi bloodstream forms and mouse peritoneal macrophages (1978) Acta trop., 35, pp. 209-219
  • Araujo-Jorge, De Souza, Effect of carbohydrates, periodate and enzymes in the process of endocytosis of Trypanosoma cruzi by macrophages (1984) Acta trop., 41, pp. 17-28
  • Batlle, Llambias, Wider de Xifra, Tigier, Porphyrin biosynthesis in the soybean callus tissue system. XV. The effect of growth conditions (1975) Int. J. Biochem., 6, p. 591
  • Beale, 5-Aminolevulinic acid in plants its biosynthesis regulation and role in plastic development (1978) Annual Review of Plant Physiology, 29, pp. 95-120
  • Brener, Biology of Trypanosoma cruzi (1973) Annu. Rev. Microbiol., 27, pp. 347-382
  • Brocklehurst, A Wider de Xifra, Batlle, The measurement of erythrocyte uroporphyrinogen I synthesis in the diagnosis of latent and acute intermittent porphyria (1980) Int. J. Biochem., 12, pp. 791-793
  • Chang, Sassa, Heme biosynthesis in bacterium-protozoan symbiosis: enzymic defects in host hemoflagellates and complemental role of their intracellular symbiotes (1975) Proc. natn. Acad. Sci. U.S.A., 72, pp. 2979-2983
  • Camargo, Growth and differentiation of Trypanosoma cruzi. I. Origin of metacyclic trypanosomes in liquid media (1964) Rev. Inst. Med. Trop. São Paulo, 6, pp. 63-100
  • Docampo, Stoppani, Generation of superoxide anion and hydrogen peroxide induced by nifurtimox in Trypanosoma cruzi (1979) Archs Biochem. Biophys., 197, pp. 317-321
  • Dörnermann, Senger, The synthesis and properties of 45-dioxovaleric acid a possible intermediate in the biosynthesis of 5-aminolevulinic acid and its in vivo formation in Scenedesmus obliquos (1980) Biochimica et Biophysica Acta (BBA) - General Subjects, 628, pp. 35-45
  • Gaugham, Krassner, Hemin deprivation in culture stages of the hemoflagellate Leishmania tarentolae (1971) Comp. Biochem. Physiol., 39 B, pp. 5-18
  • Hutner, Bacchi, Baker, Nutrition of the Kinetoplastida (1979) Biology of the Kinetoplastida, pp. 654-691. , W.H.R. Lumsden, D.A. Evans, Academic Press, New York, No. 2
  • Jensen, Thofern, Chloramin (ferroporphyrin chloride) als bakterienurichsstoff (1953) Naturforshung, 86, pp. 599-607
  • Kikuchi, Kumar, Talmadge, Shemin, The enzymic synthesis of α-aminolevulinic acid (1958) J. biol. Chem., 233, pp. 1214-1219
  • Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275
  • Lwoff, The nutrition of parasitic flagellates (Trypanosomidae, Trichomonadinae) (1951) Biochemistry and Physiology of Protozoa, pp. 131-182. , A. Lwoff, Academic Press, New York
  • Mauzerall, Granick, The occurrence and determination of ALA and PBG in urine (1956) J. biol. Chem., 219, p. 435
  • Melo, Brener, Tissue tropism of different Trypanosoma cruzi strains (1978) J. Parasitol., 64, pp. 475-482
  • Meirelles, Araujo-Jorge, de Souza, Interaction of epimastigote and trypomastigote forms of Trypanosoma cruzi with chicken macrophages in vitro (1980) Parasitology, 81, pp. 373-381
  • Opperdoes, Borst, Bakker, Leene, Localization of glycerol-3-phosphate oxidase in the mitochondrion and NAD-linked glycerol-3-phosphate dehydrogenase in the microbodies of the bloodstream form of Trypanosoma brucei (1977) Eur. J. Biochem., 76, pp. 13-29
  • Porra, Jones, Studies on ferrochelatase I. Assay and properties of ferrochelatase from a pig liver mitochondrial extract (1963) Biochem. J., 87, pp. 181-185
  • Salzman, Stella, Wider de Xifra, Batlle, Docampo, Stoppani, Porphyrin biosynthesis in parasitic hemoflagellates functional and defective enzymes in Trypanosoma cruzi (1982) Comp. Biochem. Physiol, 72 B, pp. 633-667
  • Warren, Metabolism of Schizotrypanum cruzi Chagas I. Effect of culture age and substrate concentration on respiratory rate (1960) J. Parasitol., 46, pp. 529-539

Citas:

---------- APA ----------
Salzman, T.A., Batlle, A.M.D.C. & de Souza, W. (1986) . Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 83(1), 57-61.
http://dx.doi.org/10.1016/0305-0491(86)90331-7
---------- CHICAGO ----------
Salzman, T.A., Batlle, A.M.D.C., de Souza, W. "Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 83, no. 1 (1986) : 57-61.
http://dx.doi.org/10.1016/0305-0491(86)90331-7
---------- MLA ----------
Salzman, T.A., Batlle, A.M.D.C., de Souza, W. "Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 83, no. 1, 1986, pp. 57-61.
http://dx.doi.org/10.1016/0305-0491(86)90331-7
---------- VANCOUVER ----------
Salzman, T.A., Batlle, A.M.D.C., de Souza, W. Heme synthesis in Trypanosoma cruzi: Influence of the strain and culture medium. 1986;83(1):57-61.
http://dx.doi.org/10.1016/0305-0491(86)90331-7