Abstract:
1. 1. The effect of S-adenosyl-l-methionine (SAM) administration to both acute and chronic lead exposed mice was investigated. 2. 2. SAM was given s.c. at different doses and for different time intervals. The best results were obtained using 20 mg SAM/kg applied daily over a period of 20-22 days. 3. 3. Results obtained in both acute and chronic lead poisoning were quite similar. 4. 4. GSH concentration in blood and liver, reduced in intoxicated animals was increased after SAM administration reaching normal values. 5. 5. Blood, liver and kidney lead content notably increased at the beginning of SAM treatment and decreased rapidly in the group receiving SAM, attaining values near control levels in 2 weeks. 6. 6. A significant recovery of blood, liver, kidney, spleen and brain δ-aminolevulic acid dehydratase (ALA-D) initially reduced in poisoned animals, was clearly produced after SAM administration. 7. 7. A clear and direct correlation between the recovery of both ALA-D activity and GSH levels and the decreased concentration of lead in tissues was observed, reinforcing our proposal that enhancement of thiol content as a result of SAM administration would facilitate the detoxification process and lead removal, consequently reversing the inactivation of the enzyme. 8. 8. We conclude that SAM therapy is beneficial in the treatment of lead intoxication. © 1985.
Registro:
Documento: |
Artículo
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Título: | S-adenosyl-l-methionine a counter to lead intoxication? |
Autor: | Paredes, S.R.; Kozicki, P.A.; Del C. Batlle, A.M. |
Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), Consejo Nacional de Investigaciones Cientificas y Técnicas (CONICET), y Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, FCEN-UBA-C, Universitaria, Pab. II, 1428, Buenos Aires, Argentina
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Palabras clave: | glutathione; porphobilinogen synthase; s adenosylmethionine; acute disease; animal; article; kidney; kinetics; lead poisoning; liver; male; metabolism; mouse; spleen; Acute Disease; Animal; Glutathione; Kidney; Kinetics; Lead Poisoning; Liver; Male; Mice; Porphobilinogen Synthase; S-Adenosylmethionine; Spleen; Support, Non-U.S. Gov't |
Año: | 1985
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Volumen: | 82
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Número: | 4
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Página de inicio: | 751
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Página de fin: | 757
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DOI: |
http://dx.doi.org/10.1016/0305-0491(85)90520-6 |
Título revista: | Comparative Biochemistry and Physiology -- Part B: Biochemistry and
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ISSN: | 03050491
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CAS: | glutathione, 70-18-8; porphobilinogen synthase, 9036-37-7; s adenosylmethionine, 29908-03-0, 485-80-3; Glutathione, 70-18-8; Porphobilinogen Synthase, EC 4.2.1.24; S-Adenosylmethionine, 29908-03-0
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v82_n4_p751_Paredes |
Referencias:
- Batlle, Bustos, Stella, Wider, Conti, Mendez, Enzyme replacement therapy in porphyrias IV. First successful human trial of δ-aminolevulinate dehydratase-loaded erythrocyte ghosts (1983) Int. J. Biochem., 15, pp. 1261-1265
- Beutler, Duron, Kelly, Improved method for the determination of blood glutathione (1963) J. Lab. clin. Med., 61, pp. 882-888
- Bevan, Bodlaender, Shemin, Mechanisms of porphobilinogen synthase (1980) Requirement of Zn2+ for enzyme activity, 255, pp. 2030-2035. , J. biol. Chem
- Bustos, Stella, Wider de Xifra, Batlle, Studies on erythrocyte aminolevulinate dehydratase I. Its purification and possible therapeutic applications (1980) Int. J. Biochem., 12, pp. 745-749
- Bustos, Stella, Wider, Batlle, Enzyme replacement therapy in porphyrias III: potential use of erythrocyte ghosts as carriers of δ-aminolevulinate dehydratase (1982) Int. J. Biochem., 15, pp. 447-452
- Bustos N. L. and Batlle A. M. del C. Enzyme replacement therapy in porphyrias V: in vivo correction of δ-aminolevulinate dehydratase defective erythrocytes in lead intoxicated animals by enzyme-loaded erythrocyte ghosts. In preparation; Dagg, Goldberg, Lochhead, Smith, The relation of lead poisoning to the acute intermittent porphyrias (1964) Q. J. Med., 34, pp. 163-182
- Dick, O'Brien, Ohnishi, Lead neuropathy 2 Random distribution of segmental demyelination among “old internodes” of myelinated fibers (1977) Journal of Neuropathology and Experimental Neurology, 36, pp. 570-575
- Dudley, Klaassen, Changes in glutathione concentration modify cadmium-induced hepatotoxity (1984) Toxic. appl. Pharmac., 72, pp. 530-538
- Espinola, Liposomas en sistemas biológicas-aplicación en el tratamients de las porfirias (1984) Ph.D. Thesis, , University of Buenos Aires
- Espinola, Wider, Stella, Batlle, Enzyme replacement therapy in porphyrias II: entrapment of δ-aminolevulinate dehydratase in liposomes (1982) Int. J. Biochem., 15, pp. 439-445
- Gillette, Mitchell, Bordie, Biochemical mechanism of drug toxicity (1974) Annual Review of Pharmacology, 14, pp. 271-289
- Giulidori, Galli-Kienle, Catto, Stramentinoli, Transmethylation, transsulfuration and aminopropylation reaction of S-adenosyl-l-methioninein vivo (1984) J. biol. Chem., 259, pp. 4206-4211
- Golter, Michaelson, Growth, behaviour and brain catecholamines in lead exposed neonatal rats: a reappraisal (1975) Science, N.Y., 187, pp. 359-361
- Hietanen, Kilpiö, Närhi, Savolainen, Vainio, Biotransformational and neurophysiological changes in rabbits exposed to lead (1980) Archs Envir. Contam. Toxicol., 9, pp. 337-347
- Hirano, Kochen, Further observations on the effects of lead implantation in rat brains (1976) Acta Neuropath. (Berlin), 34, pp. 87-93
- Howard, Human erythrocyte glutathione reductase and glucose 6-phosphate dehydrogenase activities in normal subjects and in persons exposed to lead (1974) Clin. Sci. Mol. Med., 47, pp. 515-520
- Howard, Interrelationships of glutathione reductase 5-aminolevulinic acid dehydratase, and free sulphydryl groups in the erythrocytes of normal and lead-exposed persons (1978) J. Toxicol. Env. Hlth, 4, pp. 51-57
- Kawata, Suzuki, Relation between metal and glutathione concentrations in mouse liver after cadmium, zinc or copper loading (1983) Toxicol. Lett., 15, pp. 131-137
- Kesten, Stella, Wider de Xifra, Batlle, Effect of cadmium on porphyrin biosynthesis—I (1980) Int. J. Biochem., 12, pp. 905-908
- Leeming, Donaldson, Effect of dietary methionine and lysine in the toxicity of ingested lead acetate in the chick (1984) J. Nutr., 114, pp. 2155-2159
- Lichtman, Feldman, In vitro pyrrole and porphyrin synthesis in lead poisoning and iron deficiency (1963) J. clin. Invest., 42, pp. 830-839
- Mitchell, Jollow, Potter, Gillette, Brodie, Acetaminophen-induced hepatic necrosis IV. Protective role of glutathione (1973) J. Pharmac. expl. Med., 187, pp. 211-217
- Nakao, Wada, Iano, δ-Aminolevulinic acid dehydratase activity in erythrocytes for the evaluation of lead poisoning (1968) Clinica chim. Acta, 19, pp. 319-325
- Paredes, Juknat de Geralnik, Batlle, Conti, Beneficial effect of S-adenosyl-l-methionine (SAMe) in lead intoxication (1985) Another approach to clinical therapy, 17, pp. 625-629. , Int. J. Biochem
- Percy, Schanley, Studies on haem biosynthesis in rat brain (1979) J. Neurochem., 33, pp. 1267-1274
- Roels, Buchet, Lauwerys, Sonnet, Comparison of in vivo effect of inorganic lead and cadmiun on glutathione reductase system and δ-aminolevulinate dehydratase in human erythrocytes (1975) Br. J. ind. Med., 32, pp. 181-192
- Silbergeld, Lamon, Role of altered heme synthesis in lead neurotoxicity (1980) J. Occup. Med., 22, pp. 680-684
- Stramentinoli, Pezzoli, Gallie-Kienle, Protective role of S-adenosyl-l-methionine against acetaminophen induced mortality and hepatic toxicity in mice (1979) Biochem. Pharmac., 28, pp. 3567-3571
- Tsukamoto, Yoshinaga, Sano, The role of zinc with special reference to the essential thiol groups in δ-aminolevulinic acid dehydratase of bovine liver (1979) Biochim. Biophys. Acta, 570, pp. 167-178
- Whetsell, Sassa, Bickers, Kappas, Studies on porphyrin heme synthesis in organotypic cultures of chick dorsal root ganglion I. Observations on neuronal and non-neuronal elements (1978) J. Neuropath. exp. Neurol., 37, pp. 497-507
- Williams, Griffith, Albhercht, Pirch, Hejtmancik, Effects of chronic lead treatment on some cardiovascular responses to norepinephrine in the rat (1977) Toxic. appl. Pharmac., 40, pp. 407-413
- Windebank, McCall, Hunder, Dyck, The endoneurial content of lead related to the onset and severity of segmental demyelination (1980) J. Neuropath. exp. Neurol., 39, pp. 692-699
- Zappia, Usdin, Salvatore, (1978) Biochemical and Pharmacological Roles of Adenosyl-Methionine and the Central Nervous System, , Pergamon Press, Oxford
Citas:
---------- APA ----------
Paredes, S.R., Kozicki, P.A. & Del C. Batlle, A.M.
(1985)
. S-adenosyl-l-methionine a counter to lead intoxication?. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 82(4), 751-757.
http://dx.doi.org/10.1016/0305-0491(85)90520-6---------- CHICAGO ----------
Paredes, S.R., Kozicki, P.A., Del C. Batlle, A.M.
"S-adenosyl-l-methionine a counter to lead intoxication?"
. Comparative Biochemistry and Physiology -- Part B: Biochemistry and 82, no. 4
(1985) : 751-757.
http://dx.doi.org/10.1016/0305-0491(85)90520-6---------- MLA ----------
Paredes, S.R., Kozicki, P.A., Del C. Batlle, A.M.
"S-adenosyl-l-methionine a counter to lead intoxication?"
. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 82, no. 4, 1985, pp. 751-757.
http://dx.doi.org/10.1016/0305-0491(85)90520-6---------- VANCOUVER ----------
Paredes, S.R., Kozicki, P.A., Del C. Batlle, A.M. S-adenosyl-l-methionine a counter to lead intoxication?. 1985;82(4):751-757.
http://dx.doi.org/10.1016/0305-0491(85)90520-6