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Salzman, T.A.; Stella, A.M.; Wider de Xifra, E.A.; Batlle, A.M.D.C.; Docampo, R.; Stoppani, A.O.M. "Porphyrin biosynthesis in parasitic hemoflagellates: Functional and defective enzymes in Trypanosoma cruzi" (1982) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 72(4):663-667
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Abstract:

1. 1. Heme compounds are necessary as a growth factor for Trypanosoma cruzi in culture, this porphyrin requirement being due to the inability of the parasite to synthesize heme. To obtain supporting evidence for this hypothesis, an extensive study of porphyrin biosynthesis in the epimastogote form of T. cruzi (Tulahuén strain) was carried out. 2. 2. Low levels of endogenous δ-aminolevulinic acid (ALA) and porphobilinogen (PBG) were found in extracts of T. cruzi. Free porphyrins and heme contents were practically nil. 3. 3. The activity of succinyl CoA synthetase (Suc, CoA-S) was rather high and therefore non-limiting. 4. 4. Both δ-aminolevulinic acid synthetase (ALA-S) and 4,5, dioxovaleric transaminase (DOVA-T), the two enzymes forming ALA, were readily detected and their activities, although low, were of the same order. 5. 5. δ-Aminolevulinic acid dehydratase (ALA-D) activity was almost negligible and both porphobilinogenase (PBGase) and deaminase were absent or inactive. 6. 6. Heme-Synthetase (Heme-S) was totally functional. 7. 7. It is concluded that T. cruzi has lost part of its heme biosynthetic pathway, possibly due to mutations of several genes involved in the synthesis of the soluble enzymes ALA-D, PBGase, deaminase and probably others preceding Heme-S; while the particulate enzymes Suc CoA-S, ALA-S, DOVA-T and Heme-S are functional. As a consequence, the host should supply the parasite with the porphyrin substrate to form its essential heme compounds. © 1982.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in parasitic hemoflagellates: Functional and defective enzymes in Trypanosoma cruzi
Autor:Salzman, T.A.; Stella, A.M.; Wider de Xifra, E.A.; Batlle, A.M.D.C.; Docampo, R.; Stoppani, A.O.M.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), CONICET, FCEN, Ciudad Universitaria, Pab. II, 1428 Buenos Aires, Argentina
Centro de Investigaciones Bioenergéticas (CIBIERG), CONICET, FCM, Paraguay 2155, 1121 Buenos Aires, Argentina
Palabras clave:5 aminolevulinate synthase; aminolevulinate aminotransferase; aminotransferase; ammonia lyase; Ammonia Lyases; ferrochelatase; heme; porphobilinogen synthase; porphyrin; Succinate CoA Ligases; succinyl coenzyme A synthetase; animal; article; biosynthesis; enzymology; metabolism; Trypanosoma cruzi; 5-Aminolevulinate Synthetase; Ammonia-Lyases; Animal; Ferrochelatase; Heme; Porphobilinogen Synthase; Porphyrins; Succinate-CoA Ligases; Support, Non-U.S. Gov't; Transaminases; Trypanosoma cruzi
Año:1982
Volumen:72
Número:4
Página de inicio:663
Página de fin:667
DOI: http://dx.doi.org/10.1016/0305-0491(82)90523-5
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
CAS:5 aminolevulinate synthase, 9037-14-3; aminolevulinate aminotransferase, 9012-46-8; aminotransferase, 9031-66-7; ferrochelatase, 9012-93-5; heme, 14875-96-8; porphobilinogen synthase, 9036-37-7; porphyrin, 24869-67-8; succinyl coenzyme A synthetase, 37341-57-4, 9014-36-2, 9080-33-5; 5-Aminolevulinate Synthetase, EC 2.3.1.37; aminolevulinate aminotransferase, EC 2.6.1.43; Ammonia-Lyases, EC 4.3.1.; Ferrochelatase, EC 4.99.1.1; Heme, 14875-96-8; Porphobilinogen Synthase, EC 4.2.1.24; Porphyrins; Succinate-CoA Ligases, EC 6.2.1.-; Transaminases, EC 2.6.1.
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v72_n4_p663_Salzman

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Citas:

---------- APA ----------
Salzman, T.A., Stella, A.M., Wider de Xifra, E.A., Batlle, A.M.D.C., Docampo, R. & Stoppani, A.O.M. (1982) . Porphyrin biosynthesis in parasitic hemoflagellates: Functional and defective enzymes in Trypanosoma cruzi. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 72(4), 663-667.
http://dx.doi.org/10.1016/0305-0491(82)90523-5
---------- CHICAGO ----------
Salzman, T.A., Stella, A.M., Wider de Xifra, E.A., Batlle, A.M.D.C., Docampo, R., Stoppani, A.O.M. "Porphyrin biosynthesis in parasitic hemoflagellates: Functional and defective enzymes in Trypanosoma cruzi" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 72, no. 4 (1982) : 663-667.
http://dx.doi.org/10.1016/0305-0491(82)90523-5
---------- MLA ----------
Salzman, T.A., Stella, A.M., Wider de Xifra, E.A., Batlle, A.M.D.C., Docampo, R., Stoppani, A.O.M. "Porphyrin biosynthesis in parasitic hemoflagellates: Functional and defective enzymes in Trypanosoma cruzi" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 72, no. 4, 1982, pp. 663-667.
http://dx.doi.org/10.1016/0305-0491(82)90523-5
---------- VANCOUVER ----------
Salzman, T.A., Stella, A.M., Wider de Xifra, E.A., Batlle, A.M.D.C., Docampo, R., Stoppani, A.O.M. Porphyrin biosynthesis in parasitic hemoflagellates: Functional and defective enzymes in Trypanosoma cruzi. 1982;72(4):663-667.
http://dx.doi.org/10.1016/0305-0491(82)90523-5