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de Lederkremer, R.M.; Waksman, N.; de Martini, G.J.W.; Segura, E.L. "The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein" (1982) Comparative Biochemistry and Physiology -- Part B: Biochemistry and. 72(2):313-316
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Abstract:

1. 1. The complex of glycoconjugates extracted with phenol-water from culture epimastigotes of a non-infective line (To) of Trypanosoma cruzi, derived from the Tulahuén strain showed only two components by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 2. 2. The slower migrating glycoconjugate, a glycoprotein of molecular weight 45,000 was purified to apparent homogeneity. 3. 3. A content of 76% protein, 17% neutral sugars and 6% glucosamine was analysed. Galactose and mannose occur in a ratio of 3:1 as determined by gas-liquid chromatography. A trace of glucose was detected in some samples. © 1982.

Registro:

Documento: Artículo
Título:The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein
Autor:de Lederkremer, R.M.; Waksman, N.; de Martini, G.J.W.; Segura, E.L.
Filiación:Departamento de Quimica Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Instituto Fatala Chabén, Paseo Colón 568, 1063 Buenos Aires, Argentina
Palabras clave:carbohydrate; glycoprotein; animal; article; gas chromatography; isolation and purification; macromolecule; molecular weight; polyacrylamide gel electrophoresis; Trypanosoma cruzi; Animal; Carbohydrates; Chromatography, Gas; Electrophoresis, Polyacrylamide Gel; Glycoproteins; Macromolecular Systems; Molecular Weight; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1982
Volumen:72
Número:2
Página de inicio:313
Página de fin:316
DOI: http://dx.doi.org/10.1016/0305-0491(82)90052-9
Título revista:Comparative Biochemistry and Physiology -- Part B: Biochemistry and
ISSN:03050491
CAS:Carbohydrates; Glycoproteins; Macromolecular Systems
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v72_n2_p313_deLederkremer

Referencias:

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  • Dubois, Gilles, Hamilton, Rebers, Smith, Colorimetric method for determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356
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Citas:

---------- APA ----------
de Lederkremer, R.M., Waksman, N., de Martini, G.J.W. & Segura, E.L. (1982) . The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 72(2), 313-316.
http://dx.doi.org/10.1016/0305-0491(82)90052-9
---------- CHICAGO ----------
de Lederkremer, R.M., Waksman, N., de Martini, G.J.W., Segura, E.L. "The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and 72, no. 2 (1982) : 313-316.
http://dx.doi.org/10.1016/0305-0491(82)90052-9
---------- MLA ----------
de Lederkremer, R.M., Waksman, N., de Martini, G.J.W., Segura, E.L. "The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein" . Comparative Biochemistry and Physiology -- Part B: Biochemistry and, vol. 72, no. 2, 1982, pp. 313-316.
http://dx.doi.org/10.1016/0305-0491(82)90052-9
---------- VANCOUVER ----------
de Lederkremer, R.M., Waksman, N., de Martini, G.J.W., Segura, E.L. The glycoconjugates from Trypanosoma cruzi, purification and composition of a glycoprotein. 1982;72(2):313-316.
http://dx.doi.org/10.1016/0305-0491(82)90052-9