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De Siervi, A.; Rossetti, M.V.; Lezama, D.; Batlle, A.M.D.C. "Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype" (1996) Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology. 115(2):167-173
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Abstract:

Heme biosynthesis was studied in the segregants of Saccharomyces cerevisiae (DW10 tetrade 2) from D27 and D27/C6 mating, as a function of the carbon source in the growth medium and the physiological state of the cells. The effects of the HEM R+ gene on the 5-aminolevulinate synthase (ALA-S) and 5-aminolevulinate dehydratase (ALA-D) activities of heme biosynthesis in cells grown on nonfermentable and fermentable carbon sources were compared. Profiles obtained for both strains grown on a fermentable carbon source (glucose) were identical. However, in the presence of a nonfermentable carbon source (ethanol), they behave quite differently, as if the mutation could only be expressed under these growth conditions. Moreover, their behavior is similar to that found for the parental strains, indicating that for the mutant its particular behavior might be inheritedly linked to the HEM R+ gene, which in turn affects some regulatory aspects of ALA synthesis explaining its characteristic phenotype.

Registro:

Documento: Artículo
Título:Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype
Autor:De Siervi, A.; Rossetti, M.V.; Lezama, D.; Batlle, A.M.D.C.
Filiación:Ctro. Invest. sobre Porfirinas y P., CIPYP (CONICET, FCEyN-UBA), Ciudad Universitaria, 1428 Buenos Aires, Argentina
Viamonte 1881 10 A, 1056 Buenos Aires, Argentina
Palabras clave:5-aminolevulinate dehydratase; 5-aminolevulinate synthase; catabolite repression and de-repression; HEM R+ mutants; heme biosynthesis; intracellular 5-aminolevulinate acid; porphobilinogen-deaminase; Saccharomyces cerevisiae; heme; article; heme synthesis; mutant; phenotype; priority journal; Saccharomyces cerevisiae; Saccharomyces cerevisiae
Año:1996
Volumen:115
Número:2
Página de inicio:167
Página de fin:173
DOI: http://dx.doi.org/10.1016/0305-0491(96)00073-9
Título revista:Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Título revista abreviado:COMP. BIOCHEM. PHYSIOL. B BIOCHEM. MOL. BIOL.
ISSN:03050491
CODEN:CBPBB
CAS:heme, 14875-96-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03050491_v115_n2_p167_DeSiervi

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Citas:

---------- APA ----------
De Siervi, A., Rossetti, M.V., Lezama, D. & Batlle, A.M.D.C. (1996) . Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype. Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, 115(2), 167-173.
http://dx.doi.org/10.1016/0305-0491(96)00073-9
---------- CHICAGO ----------
De Siervi, A., Rossetti, M.V., Lezama, D., Batlle, A.M.D.C. "Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype" . Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology 115, no. 2 (1996) : 167-173.
http://dx.doi.org/10.1016/0305-0491(96)00073-9
---------- MLA ----------
De Siervi, A., Rossetti, M.V., Lezama, D., Batlle, A.M.D.C. "Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype" . Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, vol. 115, no. 2, 1996, pp. 167-173.
http://dx.doi.org/10.1016/0305-0491(96)00073-9
---------- VANCOUVER ----------
De Siervi, A., Rossetti, M.V., Lezama, D., Batlle, A.M.D.C. Porphyrin biosynthesis in normal and haem mutants of Saccharomyces cerevisiae. Studies on the inheritance of the HEM R+ phenotype. COMP. BIOCHEM. PHYSIOL. B BIOCHEM. MOL. BIOL. 1996;115(2):167-173.
http://dx.doi.org/10.1016/0305-0491(96)00073-9