Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Tacaribe virus (TCRV) entry occurs by receptor-mediated endocytosis. To explore the entry mechanism used by TCRV, the inhibitory effects of drugs and dominant negative (DN) constructions affecting the main endocytic pathways were analyzed. In cells lacking the human transferrin receptor (hTfR), compounds and DN proteins that impair clathrin-mediated endocytosis were shown to reduce virus internalization without affecting virion binding. In contrast, in cells expressing the hTfR, compounds that affect clathrin-mediated endocytosis did not affect TCRV infection. Destabilization of cholesterol-rich plasma membrane microdomains by treatment with nystatin was not able to block virus entry in the presence of hTfR. However methyl-β-cyclodextrin, which extracts cholesterol from cell membranes, reduced virus internalization in cells expressing the hTfR. Inhibition of dynamin and neutralization of the pH of intracellular vesicles reduced virus internalization in all cell lines tested. Taken together, these results demonstrate that in cells expressing the hTfR, TCRV internalization depends on the presence of cholesterol, dynamin and acidic intracellular vesicles, while in the rest of the cell lines analyzed, clathrin-mediated endocytosis is the main TCRV entry pathway and, as expected, depends on dynamin and acidic intracellular vesicles. These results represent an important contribution to the characterization of the arenavirus replication cycle. © 2015, Springer-Verlag Wien.

Registro:

Documento: Artículo
Título:Human transferrin receptor triggers an alternative Tacaribe virus internalization pathway
Autor:Roldán, J.S.; Martínez, M.G.; Forlenza, M.B.; Whittaker, G.R.; Candurra, N.A.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, IQUIBICEN, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), Ciudad Universitaria, Pabellón II, Piso 4, Buenos Aires 1428, Argentina
Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, United States
Albert Einstein College of Medicine, Room 515, Chanin Building, 1300 Morris Park Avenue, Bronx, NY 10461, United States
Palabras clave:CD71 antigen; cholesterol; dynamin; leukocyte antigen; transferrin receptor; animal; CHO cell line; Cricetulus; host pathogen interaction; human; metabolism; New World arenavirus; pH; physiology; virus entry; Animals; Antigens, CD; Arenaviruses, New World; CHO Cells; Cholesterol; Cricetulus; Dynamins; Host-Pathogen Interactions; Humans; Hydrogen-Ion Concentration; Receptors, Transferrin; Virus Internalization
Año:2016
Volumen:161
Número:2
Página de inicio:353
Página de fin:363
DOI: http://dx.doi.org/10.1007/s00705-015-2652-3
Título revista:Archives of Virology
Título revista abreviado:Arch. Virol.
ISSN:03048608
CODEN:ARVID
CAS:cholesterol, 57-88-5; dynamin; Antigens, CD; CD71 antigen; Cholesterol; Dynamins; Receptors, Transferrin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v161_n2_p353_Roldan

Referencias:

  • Buchmeier, M.J., de la Torre, J.C., Peters, C.J., Knipe, D.M., Howley, P.M., Arenaviridae: the viruses and their replication (2007) 5th edn. Lippincott Williams &, 2, pp. 1792-1827. , Wilkins, Philadelphia
  • Southern, P.J., Arenaviruses: the viruses and their replication (1996) Fields virology, pp. 1505-1519. , Fields BN, Knipe DM, Howley PM, (eds), 2, Lippincott-Raven, Philadelphia
  • Enria, D.A., Briggiler, A.M., Sánchez, Z., Treatment of Argentine hemorrhagic fever (2008) Antiviral Res, 78, pp. 132-139. , COI: 1:CAS:528:DC%2BD1cXjtleqtb8%3D, PID: 18054395
  • Bronze, M.S., Greenfield, R.A., Preventive and therapeutic approaches to viral agents of bioterrorism (2003) Drug Discov Today, 8, pp. 740-745. , COI: 1:CAS:528:DC%2BD3sXms1yit74%3D, PID: 12944096
  • York, J., Romanowski, V., Lu, M., Nunberg, J.H., The signal peptide of the Junin arenavirus envelope glycoprotein is myristoylated and forms an essential subunit of the mature G1-G2 complex (2004) J Virol, 78, pp. 10783-10792. , COI: 1:CAS:528:DC%2BD2cXotVemtLo%3D, PID: 15367645
  • Smelt, S.C., Borrow, P., Kunz, S., Differences in affinity of binding of lymphocytic choriomeningitis virus strains to the cellular receptor alpha-dystroglycan correlate with viral tropism and disease kinetics (2001) J Virol, 75, pp. 448-457. , COI: 1:CAS:528:DC%2BD3cXovV2juro%3D, PID: 11119613
  • Abraham, J., Kwong, J.A., Albariño, C.G., Host-species transferrin receptor 1 orthologs are cellular receptors for nonpathogenic new world clade B arenaviruses (2009) PLoS Pathog, 5, p. e1000358. , PID: 19343214
  • Radoshitzky, S.R., Abraham, J., Spiropoulou, C.F., Transferrin receptor 1 is a cellular receptor for New World haemorrhagic fever arenaviruses (2007) Nature, 446, pp. 92-96. , COI: 1:CAS:528:DC%2BD2sXit1arsLo%3D, PID: 17287727
  • Abraham, J., Corbett, K.D., Farzan, M., Structural basis for receptor recognition by New World hemorrhagic fever arenaviruses (2010) Nat Struct Mol Biol, 17, pp. 438-444. , COI: 1:CAS:528:DC%2BC3cXivVCis7o%3D, PID: 20208545
  • Martin, V.K., Droniou-Bonzom, M.E., Reignier, T., Investigation of clade B New World arenavirus tropism by using chimeric GP1 proteins (2010) J Virol, 84, pp. 1176-1182. , COI: 1:CAS:528:DC%2BC3cXisFyksL4%3D, PID: 19889757
  • Pasqual, G., Rojek, J.M., Masin, M., Old world arenaviruses enter the host cell via the multivesicular body and depend on the endosomal sorting complex required for transport (2011) PLoS Pathog, 7, p. e1002232. , COI: 1:CAS:528:DC%2BC3MXht1ChsbjF, PID: 21931550
  • Martinez, M.G., Cordo, S.M., Candurra, N.A., Characterization of Junin arenavirus cell entry (2007) J Gen Virol, 88, pp. 1776-1784. , COI: 1:CAS:528:DC%2BD2sXmsFSgt7Y%3D, PID: 17485539
  • Martinez, M.G., Cordo, S.M., Candurra, N.A., Involvement of cytoskeleton in Junin virus entry (2008) Virus Res, 138, pp. 17-25. , COI: 1:CAS:528:DC%2BD1cXhsVSjsLrN, PID: 18789362
  • Martinez, M.G., Forlenza, M.B., Candurra, N.A., Involvement of cellular proteins in Junin arenavirus entry (2009) Biotechnol J, 4, pp. 866-870. , COI: 1:CAS:528:DC%2BD1MXos1aqsLk%3D, PID: 19548229
  • McGraw, T.E., Greenfield, L., Maxfield, F.R., Functional expression of the human transferrin receptor cDNA in Chinese hamster ovary cells deficient in endogenous transferrin receptor (1987) J Cell Biol, 105, pp. 207-214. , COI: 1:CAS:528:DyaL2sXltFSgurk%3D, PID: 3611186
  • Contigiani, M.S., Sabattini, M.S., Virulencia diferencial de cepas de virus Junin por marcadores biológicos en ratones y cobayos (1977) Med (B Aires), 37, pp. 244-251
  • Linero, F.N., Scolaro, L.A., Participation of the phosphatidylinositol 3-kinase/Akt pathway in Junin virus replication in vitro (2009) Virus Res, 145, pp. 166-170. , COI: 1:CAS:528:DC%2BD1MXhtV2qsr%2FK, PID: 19595723
  • Sanchez, A., Pifat, D.Y., Kenyon, R.H., Junin virus monoclonal antibodies: characterization and cross-reactivity with other arenaviruses (1989) J Gen Virol, 70, pp. 1125-1132. , COI: 1:CAS:528:DyaL1MXktVGmtbo%3D, PID: 2471803
  • Damke, H., Baba, T., Warnock, D.E., Schmid, S.L., Induction of mutant dynamin specifically blocks endocytic coated vesicle formation (1994) J Cell Biol, 127, pp. 915-934. , COI: 1:CAS:528:DyaK2cXmslOgsrc%3D, PID: 7962076
  • Benmerah, A., Bayrou, M., Cerf-Bensussan, N., Dautry-Varsat, A., Inhibition of clathrin-coated pit assembly by an Eps15 mutant (1999) J Cell Sci, 112, pp. 1303-1311. , COI: 1:CAS:528:DyaK1MXjsVygsLg%3D, PID: 10194409
  • Oldenburg, J., Reignier, T., Flanagan, M.L., Differences in tropism and pH dependence for glycoproteins from the clade B1 arenaviruses: implications for receptor usage and pathogenicity (2007) Virology, 364, pp. 132-139. , COI: 1:CAS:528:DC%2BD2sXmtVGhtLY%3D, PID: 17397892
  • Charrel, R.N., Feldmann, H., Fulhorst, C.F., Phylogeny of New World arenaviruses based on the complete coding sequences of the small genomic segment identified an evolutionary lineage produced by intrasegmental recombination (2002) Biochem Biophys Res Commun, 296, pp. 1118-1124. , COI: 1:CAS:528:DC%2BD38Xms1WlsLY%3D, PID: 12207889
  • Flanagan, M.L., Oldenburg, J., Reignier, T., New world clade B arenaviruses can use transferrin receptor 1 (TfR1)-dependent and-independent entry pathways, and glycoproteins from human pathogenic strains are associated with the use of TfR1 (2008) J Virol, 82, pp. 938-948. , COI: 1:CAS:528:DC%2BD1cXmtFCktg%3D%3D, PID: 18003730
  • Choe, H., Jemielity, S., Abraham, J., Transferrin receptor 1 in the zoonosis and pathogenesis of New World hemorrhagic fever arenaviruses (2011) Curr Opin Microbiol, 14, pp. 476-482. , COI: 1:CAS:528:DC%2BC3MXhtVGlsLrL, PID: 21807555
  • Melikyan, G.B., HIV entry: a game of hide-and-fuse? (2014) Curr Opin Virol, 4, pp. 1-7. , COI: 1:CAS:528:DC%2BC2cXktVSrurs%3D, PID: 24525288
  • Sieczkarski, S.B., Whittaker, G.R., Influenza virus can enter and infect cells in the absence of clathrin-mediated endocytosis (2002) J Virol, 76, pp. 10455-10464. , COI: 1:CAS:528:DC%2BD38XnsV2kurk%3D, PID: 12239322
  • Iwasaki, M., Ngo, N., de la Torre, J.C., Sodium hydrogen exchangers contribute to arenavirus cell entry (2014) J Virol, 88, pp. 643-654. , PID: 24173224

Citas:

---------- APA ----------
Roldán, J.S., Martínez, M.G., Forlenza, M.B., Whittaker, G.R. & Candurra, N.A. (2016) . Human transferrin receptor triggers an alternative Tacaribe virus internalization pathway. Archives of Virology, 161(2), 353-363.
http://dx.doi.org/10.1007/s00705-015-2652-3
---------- CHICAGO ----------
Roldán, J.S., Martínez, M.G., Forlenza, M.B., Whittaker, G.R., Candurra, N.A. "Human transferrin receptor triggers an alternative Tacaribe virus internalization pathway" . Archives of Virology 161, no. 2 (2016) : 353-363.
http://dx.doi.org/10.1007/s00705-015-2652-3
---------- MLA ----------
Roldán, J.S., Martínez, M.G., Forlenza, M.B., Whittaker, G.R., Candurra, N.A. "Human transferrin receptor triggers an alternative Tacaribe virus internalization pathway" . Archives of Virology, vol. 161, no. 2, 2016, pp. 353-363.
http://dx.doi.org/10.1007/s00705-015-2652-3
---------- VANCOUVER ----------
Roldán, J.S., Martínez, M.G., Forlenza, M.B., Whittaker, G.R., Candurra, N.A. Human transferrin receptor triggers an alternative Tacaribe virus internalization pathway. Arch. Virol. 2016;161(2):353-363.
http://dx.doi.org/10.1007/s00705-015-2652-3