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Abstract:

Two Vero cell lines persistently infected with XJCl3 and Cl67 strains of Junin virus and named V3 and V7, respectively, have been characterized with respect to the presence and expression of the nucleoprotein (N) and the glycoprotein precursor (GPC) viral genes. After the acute phase of infection, where a marked CPE and high titers of virus were obtained, JV persistently infected cells became morphologically undistinguishable from Vero cells and virus production dropped to undetectable levels. V3 and V7 were resistant to the superinfection with antigenically related viruses. This fact could not be attributed to the presence of defective interfering particles or non-infectious virus in the supernatant. Expression of N was consistently detected in both cultures and accumulation of two degradation products of N was evident during the late passages. Although no G1 (main surface glycoprotein) expression was observed, a marked fusogenic capacity was detected in both cultures indicating at least, the synthesis of a GPC derived fusogenic glycoprotein. Cell lysates from V3 and V7 subjected to RT-PCR, using specific primers for N gene, or to a nested RT-PCR using specific primers for GPC (G1 region) confirmed the presence of both viral genes. No viral DNA sequences could be detected in JV persistently infected cells.

Registro:

Documento: Artículo
Título:Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus
Autor:Ellenberg, P.; Edreira, M.; Lozano, M.; Scolaro, L.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Ingenieria Genetica y Biologia Molecular, Departamento de Ciencia y Tecnologia, Universidad Nacional de Quilmes, Bernal, Argentina
Palabras clave:Animalia; DNA viruses; Junin virus; nucleocapsid protein; virus antigen; virus envelope protein; virus RNA; animal; article; biosynthesis; Cercopithecus; genetics; immunology; Junin virus; Vero cell; Animals; Antigens, Viral; Cercopithecus aethiops; Junin virus; Nucleocapsid Proteins; RNA, Viral; Vero Cells; Viral Envelope Proteins
Año:2002
Volumen:147
Número:8
Página de inicio:1543
Página de fin:1557
DOI: http://dx.doi.org/10.1007/s00705-002-0823-5
Título revista:Archives of Virology
Título revista abreviado:Arch. Virol.
ISSN:03048608
CODEN:ARVID
CAS:Antigens, Viral; Nucleocapsid Proteins; RNA, Viral; Viral Envelope Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v147_n8_p1543_Ellenberg

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Citas:

---------- APA ----------
Ellenberg, P., Edreira, M., Lozano, M. & Scolaro, L. (2002) . Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus. Archives of Virology, 147(8), 1543-1557.
http://dx.doi.org/10.1007/s00705-002-0823-5
---------- CHICAGO ----------
Ellenberg, P., Edreira, M., Lozano, M., Scolaro, L. "Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus" . Archives of Virology 147, no. 8 (2002) : 1543-1557.
http://dx.doi.org/10.1007/s00705-002-0823-5
---------- MLA ----------
Ellenberg, P., Edreira, M., Lozano, M., Scolaro, L. "Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus" . Archives of Virology, vol. 147, no. 8, 2002, pp. 1543-1557.
http://dx.doi.org/10.1007/s00705-002-0823-5
---------- VANCOUVER ----------
Ellenberg, P., Edreira, M., Lozano, M., Scolaro, L. Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus. Arch. Virol. 2002;147(8):1543-1557.
http://dx.doi.org/10.1007/s00705-002-0823-5