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Abstract:

The role of vacuolar-proton ATPase (V-H+ATPAse) on Junin virus (JV) replication was evaluated by analyzing the effect of specific inhibitors of the enzyme activity on different steps of virus multiplication cycle. The presence of the macrolide antibiotics bafilomycin A1 and concanamycin A during the first two hours of infection caused a significant reduction of extracellular infectious virus production and viral protein expression in Vero and BHK-21 cells. The inhibitory action of the compounds was mainly exerted at an early stage of the JV multiplication cycle, without affecting virus attachment to the cell but preventing virus penetration. A correlation between the inhibitory action of the compounds on intracellular compartments acidification and the reduction of JV yield was observed. The addition of concanamycin A at different times after infection indicated that the compound also interferes with the release of infectious particles to the extracellular medium. Although, intracellular transport of JV glycoproteins to the cell membrane, seems not to be affected as revealed by immunofluorescence staining. The results confirm that JV enters into the cell through the endocytic pathway as previously suggested by using lysosomotropic compounds.

Registro:

Documento: Artículo
Título:Involvement of vacuolar proton ATPase in Junin virus multiplication
Autor:Castilla, V.; Palermo, L.M.; Coto, C.E.
Filiación:Laboratorio de Virología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:bafilomycin; cell adhesion; cell vacuole; concanamycin A; endocytosis; enzyme activity; enzyme inhibition; Junin virus; proton transporting adenosine triphosphatase; virus enzyme; virus infection; virus replication; Adsorption; Animals; Anti-Bacterial Agents; Cell Line; Cell Survival; Cercopithecus aethiops; Cricetinae; Enzyme Inhibitors; Fluorescent Antibody Technique, Indirect; Junin virus; Macrolides; Protein Transport; Proton-Translocating ATPases; Vacuolar Proton-Translocating ATPases; Vero Cells; Viral Proteins; Virus Replication; Junin virus
Año:2001
Volumen:146
Número:2
Página de inicio:251
Página de fin:263
DOI: http://dx.doi.org/10.1007/s007050170173
Título revista:Archives of Virology
Título revista abreviado:Arch. Virol.
ISSN:03048608
CODEN:ARVID
CAS:Anti-Bacterial Agents; bafilomycin A1, 88899-55-2; concanamycin A, 80890-47-7; Enzyme Inhibitors; Macrolides; Proton-Translocating ATPases, EC 3.6.3.14; Vacuolar Proton-Translocating ATPases, EC 3.6.1.-; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v146_n2_p251_Castilla

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Citas:

---------- APA ----------
Castilla, V., Palermo, L.M. & Coto, C.E. (2001) . Involvement of vacuolar proton ATPase in Junin virus multiplication. Archives of Virology, 146(2), 251-263.
http://dx.doi.org/10.1007/s007050170173
---------- CHICAGO ----------
Castilla, V., Palermo, L.M., Coto, C.E. "Involvement of vacuolar proton ATPase in Junin virus multiplication" . Archives of Virology 146, no. 2 (2001) : 251-263.
http://dx.doi.org/10.1007/s007050170173
---------- MLA ----------
Castilla, V., Palermo, L.M., Coto, C.E. "Involvement of vacuolar proton ATPase in Junin virus multiplication" . Archives of Virology, vol. 146, no. 2, 2001, pp. 251-263.
http://dx.doi.org/10.1007/s007050170173
---------- VANCOUVER ----------
Castilla, V., Palermo, L.M., Coto, C.E. Involvement of vacuolar proton ATPase in Junin virus multiplication. Arch. Virol. 2001;146(2):251-263.
http://dx.doi.org/10.1007/s007050170173