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Abstract:

The action of reducing, oxidizing and thiol-alkylating agents on early steps of Junin virus (JV) multiplication in Vero cells was investigated. The presence of reducing agents during virus adsorption as well as incubation of viral particles with these compounds before infection enhanced JV infectivity. On the contrary, the thiol-alkylating agent 5, 5' dithiobis (2-nitrobenzoic acid) and the oxidizing compound potassium periodate showed an inhibitory effect, suggesting that sulfhydryl groups and certain sugar moieties of viral glycoproteins play an important role in the first steps of JV infection. Also enzymatic treatment of cell monolayers and addition of concanavalin A to cultures prior to infection suggest that cellular glycoproteins are involved in virus attachment.

Registro:

Documento: Artículo
Título:Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication
Autor:Raiger Iustman, L.J.; Castilla, V.; Meich, V.; Mersich, S.E.
Filiación:Laboratorio de Virologia, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Virologia, Depto. Quimica Biológica, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:5,5' dithiobis(2 nitrobenzoic acid); alkylating agent; concanavalin a; glycoprotein; junin virus; oxidizing agent; virus adsorption; virus infectivity; virus inhibition; virus replication; Alkylating Agents; Animals; Cercopithecus aethiops; Concanavalin A; Dithionitrobenzoic Acid; Dithiothreitol; Ficain; Junin virus; Membrane Glycoproteins; Mercaptoethanol; Oxidants; Periodic Acid; Phospholipase C; Potassium Compounds; Receptors, Virus; Reducing Agents; Sulfhydryl Reagents; Vero Cells; Virulence; Virus Replication; Junin virus
Año:1998
Volumen:143
Número:7
Página de inicio:1425
Página de fin:1432
DOI: http://dx.doi.org/10.1007/s007050050386
Título revista:Archives of Virology
Título revista abreviado:Arch. Virol.
ISSN:03048608
CODEN:ARVID
CAS:Alkylating Agents; Concanavalin A, 11028-71-0; Dithionitrobenzoic Acid, 69-78-3; Dithiothreitol, 3483-12-3; Ficain, EC 3.4.22.3; Membrane Glycoproteins; Mercaptoethanol, 60-24-2; Oxidants; Periodic Acid, 10450-60-9; Phospholipase C, EC 3.1.4.3; Potassium Compounds; potassium periodate, 7790-21-8; Receptors, Virus; Reducing Agents; Sulfhydryl Reagents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v143_n7_p1425_RaigerIustman

Referencias:

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Citas:

---------- APA ----------
Raiger Iustman, L.J., Castilla, V., Meich, V. & Mersich, S.E. (1998) . Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication. Archives of Virology, 143(7), 1425-1432.
http://dx.doi.org/10.1007/s007050050386
---------- CHICAGO ----------
Raiger Iustman, L.J., Castilla, V., Meich, V., Mersich, S.E. "Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication" . Archives of Virology 143, no. 7 (1998) : 1425-1432.
http://dx.doi.org/10.1007/s007050050386
---------- MLA ----------
Raiger Iustman, L.J., Castilla, V., Meich, V., Mersich, S.E. "Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication" . Archives of Virology, vol. 143, no. 7, 1998, pp. 1425-1432.
http://dx.doi.org/10.1007/s007050050386
---------- VANCOUVER ----------
Raiger Iustman, L.J., Castilla, V., Meich, V., Mersich, S.E. Effects of reducing, oxidizing and alkylating agents on early steps of Junin virus multiplication. Arch. Virol. 1998;143(7):1425-1432.
http://dx.doi.org/10.1007/s007050050386