Abstract:
Meliacine (MA), a peptide isolated from leaves of the high plant Melia azedarach L inhibited the multiplication of foot and mouth disease virus (FMDV) in BHK-21 cells. In this report, we establish that the MA-inhibitable process takes place within the first hour of the viral reproductive cycle. MA had no virucidal effect and did not affect adsorption and penetration of the virus in cells. In experiments with neutral red-labeled virus, it was found that MA significantly suppressed the development of photoresistance of the virus in infected cells. In untreated cultures nearly all virus which adsorbed to cells was uncoated within 1 h at 37°C, whereas in treated cultures, even after 3 h only 3% of the virus was uncoated. Labeling of BHK-21 cells with acridine orange showed that MA affects the pH of intracellular acidic vesicles. Therefore, it is concluded that MA prevents the process of uncoating of FMDV in BHK-21 cells by inhibiting vacuolar acidification. Foot and mouth disease virus (FMDV) is a pathogen that affects a wide range of cloven-hoofed animals of agricultural and economic importance throughout the world. The seven serotypes of FMDV (types O, A, C, and Asia and the South African territories types 1, 2 and 3) constitute the Aphthovirus gene of the family Picornaviridae. FMDV is a small, non-enveloped, icosahedral virus with a single-stranded, positive-sense RNA genome of approximately 8 400 nucleotides [8]. Control of the disease is carried out using inactivated vaccines [4], but the major problem in control by vaccination is the antigenic variation of the virus [9, 10].
Registro:
Documento: |
Artículo
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Título: | Inhibition of foot and mouth disease virus (FMDV) uncoating by a plant-derived peptide isolated from Melia azedarach L leaves |
Autor: | Wachsman, M.B.; Castilla, V.; Coto, C.E. |
Filiación: | Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428, Buenos Aires, Argentina
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Palabras clave: | antiviral activity; cell line; foot and mouth disease virus; physical resistance; serotype; virus genome; virus inhibition; virus replication; virus RNA; Animals; Antiviral Agents; Aphthovirus; Cell Line; Cricetinae; Peptides; Plant Extracts; Plant Leaves; Plant Proteins; Trees |
Año: | 1998
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Volumen: | 143
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Número: | 3
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Página de inicio: | 581
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Página de fin: | 590
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DOI: |
http://dx.doi.org/10.1007/s007050050314 |
Título revista: | Archives of Virology
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Título revista abreviado: | Arch. Virol.
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ISSN: | 03048608
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CODEN: | ARVID
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CAS: | Antiviral Agents; meliacin; Peptides; Plant Extracts; Plant Proteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v143_n3_p581_Wachsman |
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Citas:
---------- APA ----------
Wachsman, M.B., Castilla, V. & Coto, C.E.
(1998)
. Inhibition of foot and mouth disease virus (FMDV) uncoating by a plant-derived peptide isolated from Melia azedarach L leaves. Archives of Virology, 143(3), 581-590.
http://dx.doi.org/10.1007/s007050050314---------- CHICAGO ----------
Wachsman, M.B., Castilla, V., Coto, C.E.
"Inhibition of foot and mouth disease virus (FMDV) uncoating by a plant-derived peptide isolated from Melia azedarach L leaves"
. Archives of Virology 143, no. 3
(1998) : 581-590.
http://dx.doi.org/10.1007/s007050050314---------- MLA ----------
Wachsman, M.B., Castilla, V., Coto, C.E.
"Inhibition of foot and mouth disease virus (FMDV) uncoating by a plant-derived peptide isolated from Melia azedarach L leaves"
. Archives of Virology, vol. 143, no. 3, 1998, pp. 581-590.
http://dx.doi.org/10.1007/s007050050314---------- VANCOUVER ----------
Wachsman, M.B., Castilla, V., Coto, C.E. Inhibition of foot and mouth disease virus (FMDV) uncoating by a plant-derived peptide isolated from Melia azedarach L leaves. Arch. Virol. 1998;143(3):581-590.
http://dx.doi.org/10.1007/s007050050314