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Abstract:

The effect of glycoprotein processing and transport on Junin virus (JV) maturation was studied by using brefeldin A (BFA) and carbonyl cyanide m-chlorophenylhydrazone (CCCP), two inhibitors affecting different steps of the intracellular exocytic pathway, combined with low temperature incubation. Both compounds inhibited the multiplication of JV, strain IV4454, in Vero cells in a dose dependent manner. The addition of the compounds after several cycles of infection for a very short treatment period produced an immediate arrest on the formation of JV infectious particles, due to their effect on a late event in JV infective cycle. Extracellular and cell-associated virus yields were reduced to the same extent, suggesting that the assembly of virus particles was the blocked stage. In infected cells treated with CCCP and BFA an altered subcellular distribution of partially processed viral glycoproteins was observed, with an accumulation of JV glycoproteins at the endoplasmic reticulum and a blockade of their transport to the site of envelopment in the plasma membrane. Concomitantly, the cleavage of the precursor GPC to the mature glycoprotein GP38 was strongly inhibited when the exocytic pathway was affected. Thus, results obtained with BFA allow to conclude that the proteolytic processing of GPC probably occurs at the trans-Golgi network and this cleavage together with the glycoprotein presence at the cell surface is required for maturation of infectious JV particles.

Registro:

Documento: Artículo
Título:Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus
Autor:Candurra, N.A.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Pabellón 2, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:antiinfective agent; antivirus agent; brefeldin A; carbonyl cyanide chlorophenylhydrazone; cyclopentane derivative; glycoprotein; macrolide; protein synthesis inhibitor; proteinase; virus envelope protein; animal; article; cell fractionation; Cercopithecus; drug effect; exocytosis; growth, development and aging; Junin virus; metabolism; physiology; protein processing; rabbit; Vero cell; Animals; Anti-Bacterial Agents; Antiviral Agents; Brefeldin A; Carbonyl Cyanide m-Chlorophenyl Hydrazone; Cercopithecus aethiops; Cyclopentanes; Endopeptidases; Exocytosis; Glycoproteins; Junin virus; Macrolides; Protein Processing, Post-Translational; Protein Synthesis Inhibitors; Rabbits; Subcellular Fractions; Vero Cells; Viral Envelope Proteins; Animalia; Cercopithecus; Junin virus; Oryctolagus cuniculus
Año:1997
Volumen:142
Número:11
Página de inicio:2179
Página de fin:2193
DOI: http://dx.doi.org/10.1007/s007050050234
Título revista:Archives of Virology
Título revista abreviado:ARCH. VIROL.
ISSN:03048608
CODEN:ARVID
CAS:Anti-Bacterial Agents; Antiviral Agents; Brefeldin A, 20350-15-6; Carbonyl Cyanide m-Chlorophenyl Hydrazone, 555-60-2; Cyclopentanes; Endopeptidases, EC 3.4.-; Glycoproteins; Macrolides; Protein Synthesis Inhibitors; Viral Envelope Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03048608_v142_n11_p2179_Candurra

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Citas:

---------- APA ----------
Candurra, N.A. & Damonte, E.B. (1997) . Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus. Archives of Virology, 142(11), 2179-2193.
http://dx.doi.org/10.1007/s007050050234
---------- CHICAGO ----------
Candurra, N.A., Damonte, E.B. "Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus" . Archives of Virology 142, no. 11 (1997) : 2179-2193.
http://dx.doi.org/10.1007/s007050050234
---------- MLA ----------
Candurra, N.A., Damonte, E.B. "Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus" . Archives of Virology, vol. 142, no. 11, 1997, pp. 2179-2193.
http://dx.doi.org/10.1007/s007050050234
---------- VANCOUVER ----------
Candurra, N.A., Damonte, E.B. Effect of inhibitors of the intracellular exocytic pathway on glycoprotein processing and maturation of Junin virus. ARCH. VIROL. 1997;142(11):2179-2193.
http://dx.doi.org/10.1007/s007050050234